The Use of CRISPR/cas9, ZFNs, TALENs in Generating Site-Specific Genome Alterations PDF Download
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Author:
Publisher: Academic Press
ISBN: 0128013346
Category : Science
Languages : en
Pages : 594
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Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Author:
Publisher: Academic Press
ISBN: 0128013346
Category : Science
Languages : en
Pages : 594
Get Book Here
Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Author: Jennifer A. Doudna
Publisher: Academic Press
ISBN: 9780128011850
Category : Medical
Languages : en
Pages : 549
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Book Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization science Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Author: United States. Congress. House. Committee on Energy and Commerce. Subcommittee on Commerce, Consumer Protection, and Competitiveness
Publisher:
ISBN:
Category :
Languages : en
Pages : 400
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Book Description
Author: Takashi Yamamoto
Publisher: Springer
ISBN: 4431552278
Category : Science
Languages : en
Pages : 206
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Book Description
This book serves as an introduction to targeted genome editing, beginning with the background of this rapidly developing field and methods for generation of engineered nucleases. Applications of genome editing tools are then described in detail, in iPS cells and diverse organisms such as mice, rats, marine invertebrates, fish, frogs, and plants. Tools that are mentioned include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR/Cas9, all of which have received much attention in recent years as breakthrough technologies. Genome editing with engineered nucleases allows us to precisely change the target genome of living cells and is a powerful way to control functional genes. It is feasible in almost all organisms ranging from bacteria to plants and animals, as well as in cultured cells such as ES and iPS cells. Various genome modifications have proven successful, including gene knockout and knock-in experiments with targeting vectors and chromosomal editing. Genome editing technologies hold great promise for the future, for example in biomedical research, clinical medicine, and generation of crops and livestock with desirable traits. A wide range of readers will find this book interesting, and with its focus on applications in a variety of organisms and cells, the book will be valuable for life scientists in all fields.
Author: Jose Cibelli
Publisher: Academic Press
ISBN: 0123865425
Category : Science
Languages : en
Pages : 585
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Book Description
Principles of Cloning, Second Edition is the fully revised edition of the authoritative book on the science of cloning. The book presents the basic biological mechanisms of how cloning works and progresses to discuss current and potential applications in basic biology, agriculture, biotechnology, and medicine. Beginning with the history and theory behind cloning, the book goes on to examine methods of micromanipulation, nuclear transfer, genetic modification, and pregnancy and neonatal care of cloned animals. The cloning of various species—including mice, sheep, cattle, and non-mammals—is considered as well. The Editors have been involved in a number of breakthroughs using cloning technique, including the first demonstration that cloning works in differentiated cells done by the Recipient of the 2012 Nobel Prize for Physiology or Medicine – Dr John Gurdon; the cloning of the first mammal from a somatic cell – Drs Keith Campbell and Ian Wilmut; the demonstration that cloning can reset the biological clock - Drs Michael West and Robert Lanza; the demonstration that a terminally differentiated cell can give rise to a whole new individual – Dr Rudolf Jaenisch and the cloning of the first transgenic bovine from a differentiated cell – Dr Jose Cibelli. The majority of the contributing authors are the principal investigators on each of the animal species cloned to date and are expertly qualified to present the state-of-the-art information in their respective areas. First and most comprehensive book on animal cloning, 100% revised Describes an in-depth analysis of current limitations of the technology and research areas to explore Offers cloning applications on basic biology, agriculture, biotechnology, and medicine
Author: David Eddings
Publisher: Del Rey
ISBN: 0345367693
Category : Fiction
Languages : en
Pages : 450
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Book Description
From the #1 New York Times bestselling author of the Belegariad and Mallorean series comes the first novel in a series imbued with magic and adventure. Sparhawk, Pandion Knight and Queen’s Champion, returned to Elenia after ten years of exile, only to find his young Queen Ehlana trapped in a block of ensorcelled crystal. Only the great sorcery of Sephrenia, ageless instructor of magic, kept her alive—but the spell would last only a year, and its cost was tragically high. Now a Prince Regent ruled Elenia, the puppet of Annias, ambitious Primate of the Church who planned to seize power over all the land. As Sparhawk and Sephrenia set out to find a cure for Ehlana, Sephrenia revealed that there was only one person in the west who could defeat the evil plots against Ehlana. That person was Sparhawk.
Author: Essam M. Abdelalim
Publisher: Springer
ISBN: 3319332708
Category : Medical
Languages : en
Pages : 276
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Book Description
This volume explores recent advances in the use of pluripotent stem cells (PSCs) and adult stem cells (ASCs) in basic and clinical applications. The chapters discuss use of PSCs for drug screening, genome editing, modeling of kidney, motor neuron diseases, and diabetes as well as their application in cancer; ASCs are discussed in the contexts of banking of umbilical cord stem cells, use of multipotential stromal cells (MSCs) for bone repair, cellular interactions during fracture repair stages, and therapeutic applications of neural crest stem cells and lung stem cells. The text is organized by sections dealing with PSCs and ASCs specifically, presenting the reader with a comprehensive examination of both these forms of stem cells. Expertly authored and drawing from a wealth of international perspectives, Recent Advances in Stem Cells: From Basic Research to Clinical Applications presents a succinct yet detailed review of cutting-edge research in this rapidly expanding field. This installment of Springer’s Stem Cell Biology and Regenerative Medicine series is essential reading for academics, researchers, and clinicians in the fields of cell biology, genetics, nephrology, osteology, oncology, and pulmonology.
Author: Danny E. Miller
Publisher: John Wiley & Sons
ISBN: 1118086929
Category : Science
Languages : en
Pages : 309
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Book Description
GENETIC THEORY AND ANALYSIS Understand and apply what drives change of characteristic genetic traits and heredity Genetics is the study of how traits are passed from parents to their offspring and how the variation in those traits affects the development and health of the organism. Investigating how these traits affect the organism involves a diverse set of approaches and tools, including genetic screens, DNA and RNA sequencing, mapping, and methods to understand the structure and function of proteins. Thus, there is a need for a textbook that provides a broad overview of these methods. Genetic Theory and Analysis meets this need by describing key approaches and methods in genetic analysis through a historical lens. Focusing on the five basic principles underlying the field—mutation, complementation, recombination, segregation, and regulation—it identifies the full suite of tests and methodologies available to the geneticist in an age of flourishing genetic and genomic research. This second edition of the text has been updated to reflect recent advances and increase accessibility to advanced undergraduate students. Genetic Theory and Analysis, 2nd edition readers will also find: Detailed treatment of subjects including mutagenesis, meiosis, complementation, suppression, and more Updated discussion of epistasis, mosaic analysis, RNAi, genome sequencing, and more Appendices discussing model organisms, genetic fine-structure analysis, and tetrad analysis Genetic Theory and Analysis is ideal for both graduate students and advanced undergraduates undertaking courses in genetics, genetic engineering, and computational biology.
Author: Hilaire Hiler
Publisher:
ISBN:
Category : Color
Languages : en
Pages : 94
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Book Description
Author: Santosh Kumar Upadhyay
Publisher: John Wiley & Sons
ISBN: 1119672406
Category : Science
Languages : en
Pages : 416
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Book Description
In recent years, significant advancements have been made in the management of nutritional deficiency using genome engineering—enriching the nutritional properties of agricultural and horticultural crop plants such as wheat, rice, potatoes, grapes, and bananas. To meet the demands of the rapidly growing world population, researchers are developing a range of new genome engineering tools and strategies, from increasing the nutraceuticals in cereals and fruits, to decreasing the anti-nutrients in crop plants to improve the bioavailability of minerals and vitamins. Genome Engineering for Crop Improvement provides an up-to-date view of the use of genome editing for crop bio-fortification, improved bioavailability of minerals and nutrients, and enhanced hypo-allergenicity and hypo-immunogenicity. This volume examines a diversity of important topics including mineral and nutrient localization, metabolic engineering of carotenoids and flavonoids, genome engineering of zero calorie potatoes and allergen-free grains, engineering for stress resistance in crop plants, and more. Helping readers deepen their knowledge of the application of genome engineering in crop improvement, this book: Presents genetic engineering methods for developing edible oil crops, mineral translocation in grains, increased flavonoids in tomatoes, and cereals with enriched iron bioavailability Describes current genome engineering methods and the distribution of nutritional and mineral composition in important crop plants Offers perspectives on emerging technologies and the future of genome engineering in agriculture Genome Engineering for Crop Improvement is an essential resource for academics, scientists, researchers, agriculturalists, and students of plant molecular biology, system biology, plant biotechnology, and functional genomics.
