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Author: Bor-Sen Chen
Publisher: CRC Press
ISBN: 1466592710
Category : Mathematics
Languages : en
Pages : 216
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Book Description
This book develops a rational design and systematic approach to construct a gene network with desired behaviors. In order to achieve this goal, the registry of standard biological parts and experimental techniques are introduced at first. Then these biological components are characterized by a standard modeling method and collected in the component
Author: Bor-Sen Chen
Publisher: CRC Press
ISBN: 1466592710
Category : Mathematics
Languages : en
Pages : 216
Get Book Here
Book Description
This book develops a rational design and systematic approach to construct a gene network with desired behaviors. In order to achieve this goal, the registry of standard biological parts and experimental techniques are introduced at first. Then these biological components are characterized by a standard modeling method and collected in the component
Author: Wilfried Weber
Publisher: Humana Press
ISBN: 9781493962242
Category : Science
Languages : en
Pages : 408
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Book Description
This book shows how to design and build synthetic gene networks in different host backgrounds. Coverage includes concepts for devising synthetic gene networks and application of mathematical models to the predictable engineering of desired network features.
Author: Vijai Singh
Publisher: Springer Nature
ISBN: 9811500819
Category : Science
Languages : en
Pages : 349
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Book Description
This book addresses the design of emerging conceptual tools, technologies and systems including novel synthetic parts, devices, circuits, oscillators, biological gates, and small regulatory RNAs (riboregulators and riboswitches), which serve as versatile control elements for regulating gene expression. Synthetic biology, a rapidly growing field that involves the application of engineering principles in biology, is now being used to develop novel systems for a wide range of applications including diagnostics, cell reprogramming, therapeutics, enzymes, vaccines, biomaterials, biofuels, fine chemicals and many more. The book subsequently summarizes recent developments in technologies for assembling synthetic genomes, minimal genomes, synthetic biology toolboxes, CRISPR-Cas systems, cell-free protein synthesis systems and microfluidics. Accordingly, it offers a valuable resource not only for beginners in synthetic biology, but also for researchers, students, scientists, clinicians, stakeholders and policymakers interested in the potential held by synthetic biology.
Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309219396
Category : Science
Languages : en
Pages : 570
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Book Description
Many potential applications of synthetic and systems biology are relevant to the challenges associated with the detection, surveillance, and responses to emerging and re-emerging infectious diseases. On March 14 and 15, 2011, the Institute of Medicine's (IOM's) Forum on Microbial Threats convened a public workshop in Washington, DC, to explore the current state of the science of synthetic biology, including its dependency on systems biology; discussed the different approaches that scientists are taking to engineer, or reengineer, biological systems; and discussed how the tools and approaches of synthetic and systems biology were being applied to mitigate the risks associated with emerging infectious diseases. The Science and Applications of Synthetic and Systems Biology is organized into sections as a topic-by-topic distillation of the presentations and discussions that took place at the workshop. Its purpose is to present information from relevant experience, to delineate a range of pivotal issues and their respective challenges, and to offer differing perspectives on the topic as discussed and described by the workshop participants. This report also includes a collection of individually authored papers and commentary.
Author: Fuqing Wu
Publisher:
ISBN:
Category : Gene regulatory networks
Languages : en
Pages : 0
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Book Description
Synthetic gene networks have evolved from simple proof-of-concept circuits to complex therapy-oriented networks over the past fifteen years. This advancement has greatly facilitated expansion of the emerging field of synthetic biology. Multistability is a mechanism that cells use to achieve a discrete number of mutually exclusive states in response to environmental inputs. However, complex contextual connections of gene regulatory networks in natural settings often impede the experimental establishment of the function and dynamics of each specific gene network. In this work, diverse synthetic gene networks are rationally designed and constructed using well-characterized biological components to approach the cell fate determination and state transition dynamics in multistable systems. Results show that unimodality and bimodality and trimodality can be achieved through manipulation of the signal and promoter crosstalk in quorum-sensing systems, which enables bacterial cells to communicate with each other. Moreover, a synthetic quadrastable circuit is also built and experimentally demonstrated to have four stable steady states. Experiments, guided by mathematical modeling predictions, reveal that sequential inductions generate distinct cell fates by changing the landscape in sequence and hence navigating cells to different final states. Circuit function depends on the specific protein expression levels in the circuit. We then establish a protein expression predictor taking into account adjacent transcriptional regions' features through construction of 120 synthetic gene circuits (operons) in Escherichia coli. The predictor's utility is further demonstrated in evaluating genes' relative expression levels in construction of logic gates and tuning gene expressions and nonlinear dynamics of bistable gene networks. These combined results illustrate applications of synthetic gene networks to understand the cell fate determination and state transition dynamics in multistable systems. A protein-expression predictor is also developed to evaluate and tune circuit dynamics.
Author: Filippo Menolascina
Publisher: Humana
ISBN: 9781071610343
Category : Medical
Languages : en
Pages : 353
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Book Description
This volume provides clear and direct protocols to implement automated Design-Build-Test-Learn (DBTL) into synthetic biology research. Chapters detail techniques to model and simulate biological systems, redesign biological systems, setting up of an automated biolaboratory, step-by-step guide on how to perform computer aided design, RNA sequencing, microfluidics -using bacterial cell free extracts, live mammalian cells, computational and experimental procedures, metabolic burden, computational techniques to predict such burden from models, and how DNA parts can be engineered in mammalian cells to sense, and respond to, and intracellular signals in general. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Synthetic Gene Circuits: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Bor-Sen Chen
Publisher:
ISBN: 9781306868075
Category :
Languages : en
Pages : 216
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Book Description
This book develops a rational design and systematic approach to construct a gene network with desired behaviors. In order to achieve this goal, the registry of standard biological parts and experimental techniques are introduced at first. Then these biological components are characterized by a standard modeling method and collected in the component libraries, which can be efficiently reused in engineering synthetic gene networks. Based on the system theory, some design specifications are provided to engineer the synthetic gene networks to robustly track the desired trajectory by employing the component libraries.
Author: Jamie Davies
Publisher:
ISBN: 019884154X
Category :
Languages : en
Pages : 137
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Book Description
Written primarily for mid-to-upper level undergraduates, this primer will introduce students to topics at the forefront of the subject that are being applied to probe biological problems, or to address the most pressing issues facing society. These topics will include those that form thecornerstone of contemporary research, helping students to make the transition to active researcher.This primer introduces the challenges and opportunities of applying synthetic biological techniques to mammalian cells, tissues, and organisms. It covers the special features that make engineering mammalian systems different from engineering bacteria, fungi, and plants, and provides an overview ofcurrent techniques. A variety of cutting-edge examples illustrate the different purposes of mammalian synthetic biology, including pure biomedical research, drug production, tissue engineering, and regenerative medicine.
Author: National Academies of Sciences, Engineering, and Medicine
Publisher: National Academies Press
ISBN: 0309465184
Category : Technology & Engineering
Languages : en
Pages : 189
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Book Description
Scientific advances over the past several decades have accelerated the ability to engineer existing organisms and to potentially create novel ones not found in nature. Synthetic biology, which collectively refers to concepts, approaches, and tools that enable the modification or creation of biological organisms, is being pursued overwhelmingly for beneficial purposes ranging from reducing the burden of disease to improving agricultural yields to remediating pollution. Although the contributions synthetic biology can make in these and other areas hold great promise, it is also possible to imagine malicious uses that could threaten U.S. citizens and military personnel. Making informed decisions about how to address such concerns requires a realistic assessment of the capabilities that could be misused. Biodefense in the Age of Synthetic Biology explores and envisions potential misuses of synthetic biology. This report develops a framework to guide an assessment of the security concerns related to advances in synthetic biology, assesses the levels of concern warranted for such advances, and identifies options that could help mitigate those concerns.
Author: Ari Edward Friedland
Publisher:
ISBN:
Category :
Languages : en
Pages : 142
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Book Description
Abstract: Synthetic gene networks can be constructed to emulate digital circuits and devices, giving one the ability to program and design cells with some of the principles of modern computing. A counter is one such device that would enable a new type of memory and allow for complex synthetic programming and novel behaviors. Here we report a synthetic genetic counter in Escherchia coli that counts up to three identical induction events, expressing a unique protein species to signify each number. The counter is comprised of a transcriptional cascade in which transcription is de-coupled from translation by riboregulators. The modularity of this device permits counting of varied user-defined inputs and its open-ended architecture provides a potent biotechnology platform for counting higher numbers, which is supported by mathematical modeling.
