Synthesis and Evaluation of Silica Hydride-based Fluorinated Stationary Phases PDF Download
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Author: Kavita Vipool Prajapati
Publisher:
ISBN:
Category : Amino acids
Languages : en
Pages : 178
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Book Description
Silica-based fluorinated bonded stationary phases have shown enhanced selectivity with altered elution orders for molecules differing in hydrophobicity and hydrophilicity in comparison with C8 and C18 reversed phase columns. Hence, two novel silica hydride-based fluorinated bonded phases have been synthesized using a hydrosilation procedure to exploit fluorine-based unique selectivity for polar basic metabolites. Bonded moieties have been characterized by elemental and spectral analyses. Silica hydride-based aqueous normal phase (ANP) chromatography has retention behavior similar to normal phase chromatography, except for the use of water as a part of the binary solvent (>60 % acetonitrile : water). In ANP, a higher percentile of nonpolar mobile phase shows increased retention for acids and bases, and nonpolar solutes can also be retained as in reversed phase chromatography (RPC). The synergistic effects of fluorinated phases' altered selectivity and aqueous normal phase retentivity have been explored for small polar metabolites using high performance liquid chromatography (HPLC) coupled with several detectors. Hydride-based fluorinated stationary phases showed good stability and remarkable reproducibility in retention time with %RSD
Author: Kavita Vipool Prajapati
Publisher:
ISBN:
Category : Amino acids
Languages : en
Pages : 178
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Book Description
Silica-based fluorinated bonded stationary phases have shown enhanced selectivity with altered elution orders for molecules differing in hydrophobicity and hydrophilicity in comparison with C8 and C18 reversed phase columns. Hence, two novel silica hydride-based fluorinated bonded phases have been synthesized using a hydrosilation procedure to exploit fluorine-based unique selectivity for polar basic metabolites. Bonded moieties have been characterized by elemental and spectral analyses. Silica hydride-based aqueous normal phase (ANP) chromatography has retention behavior similar to normal phase chromatography, except for the use of water as a part of the binary solvent (>60 % acetonitrile : water). In ANP, a higher percentile of nonpolar mobile phase shows increased retention for acids and bases, and nonpolar solutes can also be retained as in reversed phase chromatography (RPC). The synergistic effects of fluorinated phases' altered selectivity and aqueous normal phase retentivity have been explored for small polar metabolites using high performance liquid chromatography (HPLC) coupled with several detectors. Hydride-based fluorinated stationary phases showed good stability and remarkable reproducibility in retention time with %RSD
Author: Prema Gangadhara
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 136
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Book Description
The silanization/hydrosilation process was developed to overcome some of the drawbacks associated with the use of organosilane reagents for the synthesis of high pressure liquid chromatography (HPLC) stationary phases. The first step is called silanization, in which silica or other oxides react with triethoxysilane (TES), resulting in the formation of a silica hydride surface. Endcapping the stationary phase using trimethylchlorosilane (TMCS) and hexamethyldisilazane (HMDS) eliminates the unreacted and accessible silanols on the silica hydride intermediate. This research employed the modification of bare silica to silica hydride via silanization. Endcapping after silanization eliminated accessible and unreacted silanols. Endcapping was confirmed by using diffuse reflectance infrared Fourier transform (DRIFT) spectroscopy. The stationary phase was characterized for its hydrophobicity and silanophilicity properties. The effectiveness of the stationary phase was determined for its aqueous normal phase and reversed-phase chromatographic behavior. The performance was compared with the performance of C8 silica hydride column under similar conditions. This research confirmed that endcapped columns could be used in the aqueous normal phase and reversed-phase modes. Endcapping increased the hydrophobicity of the surface, which in turn increased the efficiency of separation for nonpolar solutes.
Author:
Publisher: ScholarlyEditions
ISBN: 1464966508
Category : Technology & Engineering
Languages : en
Pages : 691
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Book Description
Issues in Chemical, Biological, and Medical Engineering: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Chemical, Biological, and Medical Engineering. The editors have built Issues in Chemical, Biological, and Medical Engineering: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Chemical, Biological, and Medical Engineering in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Chemical, Biological, and Medical Engineering: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author: Amber Dawn Moore
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Author:
Publisher:
ISBN:
Category : Chemistry
Languages : en
Pages : 2616
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Book Description
Author: Davy Guillarme
Publisher: Royal Society of Chemistry
ISBN: 1849733880
Category : Science
Languages : en
Pages : 467
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Book Description
This book presents a comprehensive overview of UHPLIC and is essential reading to newcomers to field and postgradutes and an essential handbook for experienced users of the technique.
Author: Jonathan G. Rudick
Publisher: Frontiers Media SA
ISBN: 2889634841
Category :
Languages : en
Pages : 100
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Book Description
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Author: Stavros Kromidas
Publisher: John Wiley & Sons
ISBN: 3527336818
Category : Science
Languages : en
Pages : 378
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Book Description
The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.
Author: R. G. Barnes
Publisher:
ISBN:
Category : Energy storage
Languages : en
Pages : 336
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Book Description
Ames Laboratory, Iowa, USA
Author: Klaus Kopka
Publisher: MDPI
ISBN: 3038420840
Category : Medical
Languages : en
Pages : 257
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Book Description
This book is a printed edition of the Special Issue "Radiopharmaceutical Chemistry between Imaging and Radioendotherapy" that was published in Pharmaceuticals
