Synthesis and Evaluation of Four Novel Stationary Phases in High Pressure Liquid Chromatography PDF Download
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Author: Niloofar Salehi
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 129
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Book Description
High pressure liquid chromatography is one of the most frequently used methods for the separation of mixtures. Chemically modified silica hydride stationary phases, due to their unique selectivity and versatility, have been the focus of an increasing number of studies in the past several years. The objective of fabricating stationary phases with dual properties is to achieve separation of both polar and non-polar components in a single analysis. Due to an increasing demand in the fields of drug discovery, proteomics, and metabolomics to analyze numerous samples with a wide range of polarities, there is a need for more versatile stationary phases for a wide range of applications. In order to meet the above objective, in this research four novel silica hydride-based columns were synthesized using a hydrosilation procedure. The characterization of each column was done using a series of polar and nonpolar compounds by studying their aqueous normal phase and reversed-phase chromatographic behavior. Under these conditions, it turned out that two columns showed both RP and ANP behaviors. Of the remaining columns, one exhibited ANP behavior only and the other performed only in the reversed-phase mode.
Author: Niloofar Salehi
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 129
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Book Description
High pressure liquid chromatography is one of the most frequently used methods for the separation of mixtures. Chemically modified silica hydride stationary phases, due to their unique selectivity and versatility, have been the focus of an increasing number of studies in the past several years. The objective of fabricating stationary phases with dual properties is to achieve separation of both polar and non-polar components in a single analysis. Due to an increasing demand in the fields of drug discovery, proteomics, and metabolomics to analyze numerous samples with a wide range of polarities, there is a need for more versatile stationary phases for a wide range of applications. In order to meet the above objective, in this research four novel silica hydride-based columns were synthesized using a hydrosilation procedure. The characterization of each column was done using a series of polar and nonpolar compounds by studying their aqueous normal phase and reversed-phase chromatographic behavior. Under these conditions, it turned out that two columns showed both RP and ANP behaviors. Of the remaining columns, one exhibited ANP behavior only and the other performed only in the reversed-phase mode.
Author: Farnoosh Roohi
Publisher:
ISBN:
Category :
Languages : en
Pages : 228
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Author: Zhixin Jessica Tan
Publisher:
ISBN:
Category : Capillary liquid chromatography
Languages : en
Pages : 338
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Author: Subhash Chandra Dhanesar
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Author: William Garrick Etherington
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ISBN:
Category :
Languages : en
Pages :
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Author: Steven Douglas Fazio
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ISBN:
Category : Chromatographic analysis
Languages : en
Pages : 392
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Author: David B. Rusterholz
Publisher:
ISBN:
Category : Liquid chromatography
Languages : en
Pages : 26
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Author: Zhuxu Wang
Publisher:
ISBN:
Category : Chemical bonds
Languages : en
Pages : 124
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Author: Christian Geibel
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Category :
Languages : en
Pages : 0
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Chiral liquid chromatography is a field of utmost importance for a wide range of academic and industrial applications. Drug development, environmental analysis, laboratory-based diagnostics, or structure elucidation among others, strongly rely on chiral liquid chromatography nowadays. For this reason, this cumulative thesis describes the development and applications of chiral stationary phases for enantioselective (ultra-)high performance liquid chromatography ((U)HPLC). In the first part of the thesis, new chiral stationary phases (CSPs) based on anion-exchange and zwitterionic ion exchange chiral selectors were developed. The following goals were achieved during the work on this thesis: Various silica particles were evaluated for their kinetic performance optimization for enantioselective UHPLC and a deep insight into a variety of interactions between analyte and stationary phase was gained [Publication I]. The bonding chemistry and its generation by thermic or UV-driven radical starter was evaluated and systematically optimized by introducing a solvent-free photo-click immobilization strategy resulting in low-bleeding, “platform-type” stationary phases [Publication II]. Furthermore, the functionalization of bare silica, which can be seen as the starting point of the production of a CSP-based HPLC column, was varied and optimized for fast separations by the reduction of mass transfer resistance using silatranes and thus forming thin, homogeneous monolayer of thiol functionalization in a multiple-step [Publication III] and in a single-step approach [Publication IV]. The second part of the thesis focuses on the application of enantiomer separation. This includes the elaboration of the following aspects: Since chiral methods are becoming increasingly important in the fields of metabolomics and lipidomics, a guide for the chiral analysis of branched short-chain fatty acids based on polysaccharide columns was developed [Publication V]. Furthermore, the chirality of previously unknown natural compounds was elucidated [Publication VI]. A protocol for the enantiopurification of a radiotracer was established [Publication VII]. Knowledge about chiral HPLC was used for both analytical and preparative separation in the development of potential new drugs [Publications VIII and IX]. In summary, extensive research on the design, functionalization, synthesis, and evaluation of chiral stationary phases and their scope of applications has been performed.
Author: Rena M. Pomaville
Publisher:
ISBN:
Category :
Languages : en
Pages : 614
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