Supercharged NK Cells Lyse Both CSCs/poorly Differentiated and Well Differentiated Tumors PDF Download
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Author: Yash Jain
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Our previous research has shown that natural killer (NK) cells play a vital role as immune effectors in the selection and differentiation of various cancer stem cells and undifferentiated tumors. They achieve this through the direct killing and the release of IFN-[gamma] and TNF-[alpha], which inhibit tumor growth and curtail metastasis. Mechanistically, our studies have revealed that NK cells expanded by Osteoclasts display the ability to lyse CD4+ T cells while sparing CD8+ T cells, suggesting a potential selective effect of OC-activated NK cells on CD8+ T cell expansion. Consistent with these findings, we observed increased IFN-[gamma] secretion, NK cell-mediated cytotoxicity, and higher proportions of CD8+ T cells in various tissue compartments of oral tumor-bearing hu-BLT mice following immunotherapy with OC-expanded NK cells. In this study, we focused on investigating the lytic and differentiation capabilities of supercharged NK (sNK) cells in ovarian cancer cell lines (OVCAR 4 and OVCAR 8), oral cancer cell lines (OSCC and OSCSC), and a pancreatic cancer cell line (MP 2). Our results demonstrated that sNK cells exhibited greater lysing activity against both poorly differentiated and well differentiated tumors compared to pNK cells treated with IL-2. Furthermore, the supernatants of sNK cells exhibited significantly higher levels of IFN-[gamma] and induced differentiation by an increase in MHC class1 expressions in all the cell lines tested, except for OVCAR 8. Lastly, we observed a downmodulation of NKG2A and CD94 inhibitory receptors on sNK cells, suggesting potential alterations in their functional properties.
Author: Yash Jain
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Our previous research has shown that natural killer (NK) cells play a vital role as immune effectors in the selection and differentiation of various cancer stem cells and undifferentiated tumors. They achieve this through the direct killing and the release of IFN-[gamma] and TNF-[alpha], which inhibit tumor growth and curtail metastasis. Mechanistically, our studies have revealed that NK cells expanded by Osteoclasts display the ability to lyse CD4+ T cells while sparing CD8+ T cells, suggesting a potential selective effect of OC-activated NK cells on CD8+ T cell expansion. Consistent with these findings, we observed increased IFN-[gamma] secretion, NK cell-mediated cytotoxicity, and higher proportions of CD8+ T cells in various tissue compartments of oral tumor-bearing hu-BLT mice following immunotherapy with OC-expanded NK cells. In this study, we focused on investigating the lytic and differentiation capabilities of supercharged NK (sNK) cells in ovarian cancer cell lines (OVCAR 4 and OVCAR 8), oral cancer cell lines (OSCC and OSCSC), and a pancreatic cancer cell line (MP 2). Our results demonstrated that sNK cells exhibited greater lysing activity against both poorly differentiated and well differentiated tumors compared to pNK cells treated with IL-2. Furthermore, the supernatants of sNK cells exhibited significantly higher levels of IFN-[gamma] and induced differentiation by an increase in MHC class1 expressions in all the cell lines tested, except for OVCAR 8. Lastly, we observed a downmodulation of NKG2A and CD94 inhibitory receptors on sNK cells, suggesting potential alterations in their functional properties.
Author: Anahid Jewett
Publisher: Academic Press
ISBN: 0128226269
Category : Science
Languages : en
Pages : 496
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Book Description
NK Cells in Cancer Immunotherapy: Successes and Challenges explains the latest immunotherapeutic strategies, focusing on NK cells to allow the best and precise combination treatments to cancer patients. The book provides existing background knowledge in the field of immunotherapy and discusses future areas of research required to carry out cutting-edge, validated therapies. Chapters cover advances in immunotherapeutic strategies, in particular, the use of NK cells with and without T-cell therapy in the treatment of cancer. The book is a valuable resource for cancer researchers, oncologists, graduate students and those interested in learning more about novel strategies to treat cancer patients. Immunotherapy is fast becoming the method of choice for cancer therapy. Although remarkable advances have been made in the field of immunotherapy, there are significant challenges and difficulties ahead since many of the current immunotherapeutic strategies do not provide long-lasting treatment strategies, and therefore are not very effective. Covers CAR/T and CAR/NK and adoptive NK cell therapy with and without T cell therapies Discusses basic biology of NK cells and mouse models of human cancers and the role of NK cells in metastatic cancer and in cancer stem cells Encompasses information on combination therapies using check point inhibition, adoptive transfer of cytotoxic effector cells, chemotherapeutic drugs and activating and inhibitory antibodies
Author: Benjamin Bonavida
Publisher: Academic Press
ISBN: 0128243961
Category : Science
Languages : en
Pages : 540
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Book Description
Successes and Challenges of NK Immunotherapy: Increasing Anti-tumor Efficacy describes the unique therapeutic applications of NK cells to fight cancers and eliminate the bulk and subset of cancer stem cells responsible for metastasis, relapse and recurrences. The book provides information on the development, engineering, mechanisms of action, response to various preclinical models, and applications in various clinical trials. Sections cover the development of highly engineered cytotoxic NK cells, their mechanisms of action, preclinical and clinical applications, the development and application of CAR-NK cells, and new NK-drug conjugates, also emphasizing that activated NK cells can target and kill highly resistant cancer stem cells. Written by the leading experts on NK immunotherapy worldwide, this is a valuable resource for researchers, clinicians and members of the biomedical field who are interested in understanding novel and efficient therapies to fight cancers. Discusses the unique developmental applications of NK immunotherapy against cancers, which differs greatly from other types of immunotherapies Provides up-to-date and highly relevant information through chapters written by the leading researchers in the field Presents a significant number of schematic diagrams for easy understanding and reproducibility
Author: Natasha S. Barteneva
Publisher: Humana Press
ISBN: 9781493976799
Category : Science
Languages : en
Pages : 363
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Book Description
This volume is essential for anyone struggling with the cellular heterogeneity field, and it connects this new area with the latest advances in innovative techniques combined with practical advice from international experts. The chapters provide a comprehensive overview of cellular heterogeneity analysis techniques, exploring cytometry, imaging and spectroscopy methods, metabolic and molecular biological methods in tracking, as well as chromatin and cell cycle heterogeneity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cellular Heterogeneity: Methods and Protocols encourages readers to explore new ways of studying cells that will help lead to exciting new discoveries.
Author: Khawaja Husnain Haider
Publisher: Walter de Gruyter GmbH & Co KG
ISBN: 3110586916
Category : Medical
Languages : en
Pages : 340
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Book Description
This book is a compilation of the bench experience of leading experts from various research labs involved in the cutting edge area of research. The authors describe the use of stem cells both as part of the combinatorial therapeutic intervention approach and as tools (disease model) during drug development, highlighting the shift from a conventional symptomatic treatment strategy to addressing the root cause of the disease process. The book is a continuum of the previously published book entitled "Stem Cells: from Drug to Drug Discovery" which was published in 2017.
Author: Neil McKinney
Publisher:
ISBN: 9781894953986
Category : Health & Fitness
Languages : en
Pages : 568
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Book Description
Updated for 2012, this book, Dr. Neil McKinney's fourth on naturopathic oncology, is updated with the rewards of clinical practice, study, research and reader feedback over the last several years. Patients and integrative physicians will find it easier to navigate, more complete, and of real service. DO: use this book to be informed about your best options, and what to expect them to accomplish. THEN: get expert guidance from a licensed, accountable, health professional team experienced in treating cancer. Cancer is a life-threatening disease in most cases. You do not have the objectivity, experience or knowledge to make critical medical decisions alone. This is not just a legal disclaimer! Cancer is unforgiving of delays and poor choices.
Author: Edwin Choy
Publisher: MDPI
ISBN: 3906980464
Category : Connective tissues
Languages : en
Pages : 157
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Book Description
This book is a printed edition of the Special Issue "Soft Tissue and Bone Sarcoma" that was published in Cancers
Author: Robert A. Meyers
Publisher: Wiley-Blackwell
ISBN: 9783527337897
Category : Science
Languages : de
Pages : 0
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Book Description
The largest high-level encyclopedia on molecular medicine is now publishing a topical volume on Nanomedicine. The long awaited volume gives a comprehensive overview on nanomaterials in drug delivery, imaging and as therapeutics.
Author: Takehiro Izumo
Publisher: Springer
ISBN: 9812879161
Category : Medical
Languages : en
Pages : 251
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Book Description
This book provides a detailed overview of the latest innovations in respiratory endoscopy, from both diagnostic and therapeutic perspectives; each chapter focuses on one disease and the techniques for early diagnosis as well as treatment. It comprehensively covers treatment and procedures, including simultaneous X-ray fluoroscopy and its use during bronchoscopic procedures. This fast-developing technology is essential for the medical management of non-malignant and malignant diseases of the chest, especially lung cancer. Respiratory Endoscopy describes the cooperation between all the members of the healthcare team, and as such is a valuable resource not only for medical staff, but also for radiological technicians and nursing staff who contribute significantly in the care of the patients undergoing these invasive procedures. By promoting teamwork and providing practical know-how, it will improve the success and safety of respiratory endoscopy procedures.
Author: Christine L. Mummery
Publisher: Academic Press
ISBN: 0124115675
Category : Science
Languages : en
Pages : 446
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Book Description
The second edition of Stem Cells: Scientific Facts and Fiction provides the non-stem cell expert with an understandable review of the history, current state of affairs, and facts and fiction of the promises of stem cells. Building on success of its award-winning preceding edition, the second edition features new chapters on embryonic and iPS cells and stem cells in veterinary science and medicine. It contains major revisions on cancer stem cells to include new culture models, additional interviews with leaders in progenitor cells, engineered eye tissue, and xeno organs from stem cells, as well as new information on "organs on chips" and adult progenitor cells. In the past decades our understanding of stem cell biology has increased tremendously. Many types of stem cells have been discovered in tissues that everyone presumed were unable to regenerate in adults, the heart and the brain in particular. There is vast interest in stem cells from biologists and clinicians who see the potential for regenerative medicine and future treatments for chronic diseases like Parkinson's, diabetes, and spinal cord lesions, based on the use of stem cells; and from entrepreneurs in biotechnology who expect new commercial applications ranging from drug discovery to transplantation therapies. Explains in straightforward, non-specialist language the basic biology of stem cells and their applications in modern medicine and future therapy Includes extensive coverage of adult and embryonic stem cells both historically and in contemporary practice Richly illustrated to assist in understanding how research is done and the current hurdles to clinical practice
