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Author: Tohru Minamino
Publisher: Humana
ISBN: 9781493983414
Category : Science
Languages : en
Pages : 326
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Book Description
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Author: Tohru Minamino
Publisher: Humana
ISBN: 9781493983414
Category : Science
Languages : en
Pages : 326
Get Book Here
Book Description
This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Author: Tohru Minamino
Publisher:
ISBN: 9783036513386
Category :
Languages : en
Pages : 356
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Book Description
The bacterial flagellum is a supramolecular motility machinery consisting of the basal body acting as a rotary motor, the hook as a universal joint and the filament as a helical propeller. The bacterial flagellar motor composed of a rotor ring and a dozen stators is powered by an electrochemical-potential difference of specific ions across the cytoplasmic membrane and rotates in either the counterclockwise (CCW) or clockwise (CW) direction. A sensory signal transduction pathway regulates the switching between the CCW and CW states of the motor in response to environmental stimuli, allowing bacterial cells to migrate more desirable environments for their survival. The core structure of the bacterial flagellum is conserved among bacterial species. However, recent structural analyses of intact flagellar structures derived from various bacterial species by electron cryotomography and subtomogram averaging have shown that novel and divergent structures surround the core structure, suggesting that the flagellar motors have adapted to function in various environments of the habitat of bacteria. This Special Issue of Biomolecules covers recent advances in our understanding of and perspectives on the flagellar motor derived from different bacterial species.
Author: Guillaume Duménil
Publisher: Springer Nature
ISBN: 3030468860
Category : Medical
Languages : en
Pages : 141
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Book Description
This book emerges from the idea that specific physics-inspired approaches are necessary to understand different stage of bacterial physiology and the infections they cause. Many aspects of bacterial life depend on processes typically described by physical laws: The rheology of biofilms is determined by complex cohesive forces. Physical laws of diffusion are essential to all processes of bacterial metabolism. The formation of the numerous bacterial biomacromolecules require complex self-organization processes and their function are powered by potent molecular motors. Host-pathogen interactions during infection frequently occur in environments determined by fluid mechanics. In this book, different chapters represent research at the interface between microbiology and physics. Topics range from intracellular organization to cell-cell interactions. A good part of the book is devoted to mechanical forces, which are involved in the function of elaborate bacterial nanomachines, chromosome segregation, and cell division. The effect of bacterial toxins provides an example of the alteration of cellular membrane properties by bacteria. Symmetrically, histones from mammalian cells alter bacterial membranes as a defense mechanism during infection. The editors of this book, Guillaume Duménil and Sven van Teeffelen, have selected researchers at the forefront of research in physical microbiology to provide the most recent view in this fast-moving field. The contents of this book are designed to be accessible for scientists with training in biology and for scientists with training in physics. The objective is to provide a fresh perspective on microbiology and infection by highlighting recent multidisciplinary research and favor rapid advances at this fruitful interface.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Maria Teresa Mascellino
Publisher: BoD – Books on Demand
ISBN: 1789234441
Category : Science
Languages : en
Pages : 132
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Book Description
This book deals with the microorganism Salmonella. This bacterium is well known for a long time, being involved in systemic (typhus and paratyphus infections) and nonsystemic diseases such as food poisoning. Major and minor Salmonellae are widespread worldwide in developing countries and industrialized areas, respectively. In 2015, about 3576 Salmonella strains have been isolated from human infections in Italy. S. typhimurium and S. enteritidis are the most prevalent serotypes and represent 80% of cases of infections over the last 10 years. The antibiotic susceptibility decrease over the last decades is a big issue in the management of this bacterium, once considered easy to treat. The use of antibiotic combinations in order to overcome the microorganism resistance should be hoped.
Author: A. Perez-Miravete
Publisher: Springer
ISBN: 9781468419641
Category : Medical
Languages : en
Pages : 301
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Book Description
Organisms are constantly being bombarded by stimuli in their envi ronment (and also by internal stimuli), and a common way of responding is by movement. This is an aspect of irritability, or excitability, or behaviour. Response to stimuli by movement is found in all organisms: it represents one of the universalities of biology. Yet at the molecular level it is one of the least understood of biological phenomena. Micro-organisms are no exception. If motile, they respond to stimuli by active movement (taxis); if sessile, they respond by growth movements (tropisms). Responses by movement are known among micro-organisms to such stimuli as chemicals, electric current, gravity, light, temperature, touch, and vibrations. The behaviour of micro-organisms is an exciting subject, first of all for its own sake, but in addition because it may reveal facts and concepts that are applicable to understanding behaviour in more complicated organisms (even us) and because it may, help to understand the movement of cells and tissues during differentiation and development of higher plants and animals.
Author: J. Robin Harris
Publisher: Springer Nature
ISBN: 3030589714
Category : Science
Languages : en
Pages : 580
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Book Description
This book covers important topics such as the dynamic structure and function of the 26S proteasome, the DNA replication machine: structure and dynamic function and the structural organization and protein–protein interactions in the human adenovirus capsid, to mention but a few. The 18 chapters included here, written by experts in their specific field, are at the forefront of scientific knowledge. The impressive integration of structural data from X-ray crystallography with that from cryo-electron microscopy is apparent throughout the book. In addition, functional aspects are also given a high priority. Chapter 1 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author: Thomas Dean Pollard
Publisher:
ISBN: 9781621820161
Category : Cell membranes
Languages : en
Pages : 0
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Book Description
The cytoskeleton is the intracellular filament system that controls the morphology of a cell, allows it to move, and provides trafficking routes for intracellular transport. It comprises three major filament systems-actin, microtubules, and intermediate filaments-along with a host of adaptors, regulators, molecular motors, and additional structural proteins. This textbook presents a comprehensive and up-to-date view of the cytoskeleton, cataloguing its many different components and explaining how they are functionally integrated in different cellular processes. It starts by laying out the basic molecular hardware, before describing in detail how these components are assembled in cells and linked to neighboring cells and the extracellular matrix to maintain tissue architecture. It then surveys the roles of the cytoskeleton in processes such as intracellular transport, cell motility, signal transduction, and cell division. The book is thus essential reading for students learning about intracellular structure. It also represents a vital reference for all cell and developmental biologists working in this field.
Author: Shin-Ichi Aizawa
Publisher: Elsevier
ISBN: 0124172830
Category : Science
Languages : en
Pages : 142
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Book Description
The Flagellar World is a unique publication. The product of years of research and data collection by the author, this book is a pictorial guide to flagella in a variety of organisms. Each EM image is accompanied by a short description of the system in each organism. These never-before-seen pictures represent a wide variety of flagella, including Legionella pneumophila, Escherichia coli, Pseudomonas aeruginosa, and many others. Researchers in microbiology, immunology, and parasitology will find this a fascinating and useful resource. - A unique publication with many never before seen EM images of flagella - A historical document of years of research on flagella - Fills a specific niche that shows flagella in several varied organisms
Author: Eric Hanssen
Publisher: Springer
ISBN: 3319689975
Category : Science
Languages : en
Pages : 339
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Book Description
This book highlights important techniques for cellular imaging and covers the basics and applications of electron tomography and related techniques. In addition, it considers practical aspects and broadens the technological focus by incorporating techniques that are only now becoming accessible (e.g. block face imaging). The first part of the book describes the electron microscopy 3D technique available to scientists around the world, allowing them to characterize organelles, cells and tissues. The major emphasis is on new technologies like scanning transmission electron microscopy (STEM) tomography, though the book also reviews some of the more proven technologies like electron tomography. In turn, the second part is dedicated to the reconstruction of data sets, signal improvement and interpretation
