Author: Dagmar Klostermeier
Publisher: Walter de Gruyter
ISBN: 3110284952
Category : Science
Languages : en
Pages : 432
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Book Description
While structure-function relationships of proteins have been studied for a long time, structural studies of RNA face additional challenges. Nevertheless, with the continuous discovery of novel RNA molecules with key cellular functions and of novel pathways and interaction networks, the need for structural information of RNA is still increasing. This volume provides an introduction into techniques to assess structure and folding of RNA. Each chapter explains the theoretical background of one technique, and illustrates possibilities and limitations in selected application examples.
Author: Chris Baugh
Publisher:
ISBN:
Category : Protein binding
Languages : en
Pages : 348
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Book Description
Author: Nina T. Parker
Publisher:
ISBN: 9781947172791
Category : Microbiology
Languages : en
Pages : 0
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Book Description
Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Walter Ensign Hill
Publisher: ASM Press
ISBN:
Category : Science
Languages : en
Pages : 716
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Book Description
This book is recommended as a 'hitchhiker's guide to the ribosome' for everyone with an interest in translation, RNA-protein structures, and macromolecular structure-function relationships Science >.
Author: Beena Mary Kadakkuzha
Publisher:
ISBN:
Category : RNA.
Languages : en
Pages : 288
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Book Description
RNA has the intrinsic property to adopt multiple conformations through very fast independent as well as coupled motions. Resolving the high-resolution structures of dynamically inter-converting states is a major challenge for traditional biophysical techniques such as NMR spectroscopy and X-ray crystallography which provide an averaged structure of well folded conformations. It has been shown that the presence of multiple conformations contributes to the ruggedness of the RNA energy landscape, and the functionally relevant conformations could be less populated or transiently present in the ensemble of structure. In two different RNA molecules; a ribozyme with an asymmetric internal loop and a protein binding RNA with a trinucleotide bulge, the conformational dynamics and the structure-function correlation are explored by time-resolved ultrafast fluorescence spectroscopy. Here, RNAs are labeled with one of the most extensively studied fluorescent nucleic acid probes 2-aminopurine, to probe the base stacking patterns in specific motifs in RNA. Base stacking interactions are the major stabilizing force in RNA and the base stacking motions contribute to the local dynamics in RNA. Chapter 1 reviews the broad picture of RNA beyond the static structures and the role of conformational dynamics in defining structure-function relationships in RNA. Chapter 2 explains the basic principles and experimental set-up of ultrafast spectroscopy approach used in our lab and a brief discussion of other spectroscopic techniques that complement the time-resolved spectroscopy. Chapter 3 discusses the conformational dynamics and heterogeneity of leadzyme. We observed a highly unique asymmetric loop structure in leadzyme that lacks orderliness in the catalytic loop. Chapter 4 reviews the heterogeneity of HIV-1 TAR RNA, where we probe the free and ligand bound TAR to capture any bound conformation in the free state that can lead to the proposal of a possible conformational capture mechanism in TAR-Tat recognition. Chapter 5 summarizes our findings and the future prospects of employing time-resolved approach to study the dynamics of biomolecules. In conclusion, we observed RNA as an ensemble of structures in different structural context rather than a single rigid static structure.
Author: Julie Yeager-Zink
Publisher:
ISBN:
Category :
Languages : en
Pages : 214
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Book Description
Author: Jan Gorodkin
Publisher:
ISBN: 9781627037099
Category : Nucleotide sequence
Languages : en
Pages : 533
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Book Description
The existence of genes for RNA molecules not coding for proteins (ncRNAs) has been recognized since the 1950's, but until recently, aside from the critically important ribosomal and transfer RNA genes, most focus has been on protein coding genes. However, a long series of striking discoveries, from RNA's ability to carry out catalytic function, to discovery of riboswitches, microRNAs and other ribo-regulators performing critical tasks in essentially all living organisms, has created a burgeoning interest in this primordial component of the biosphere. However, the structural characteristics and evolutionary constraints on RNA molecules are very different from those of proteins, necessitating development of a completely new suite of informatic tools to address these challenges. In RNA Sequence, Structure, Function: Computational and Bioinformatic Methods, expert researchers in the field describe a substantial and relevant fraction of these methodologies from both practical and computational/algorithmic perspectives. Focusing on both of these directions addresses both the biologist interested in knowing more about RNA bioinformatics as well as the bioinformaticist interested in more detailed aspects of the algorithms. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results. Thorough and intuitive, RNA Sequence, Structure, Function: Computational and Bioinformatic Methods aids scientists in continuing to study key methods and principles of RNA bioinformatics.
Author: Andrej Lupták
Publisher:
ISBN:
Category :
Languages : en
Pages : 326
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Book Description
Author: Carlos Gonzalez Oliver
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
"RNA is the only member of the central dogma of biology that is able to simultaneously store genetic information, and independently carry out a broad range of chemical reactions.As such, it is an essential molecule to all organisms, and is at the heart of current theories on the genesis of life.Understanding the origins and complex functioning of biomolecules is always arrived at through detailed understanding of molecular structures.However, broad explorations of RNA structure-function relationships are often met with challenges in scale.In humans alone, the number of functional RNA elements is hypothesized to be in the tens of thousands, and evolutionary studies of RNA structure require explorations of mutational landscapes which grow exponentially.This calls for the development of computational methods aimed at efficiently probing RNA structure data.In this thesis, we develop algorithms to address key questions in the origins, functioning, and emergent patterns of complex RNA structures.Our proposed algorithms leverage ideas from dynamic programming, machine learning, and network theory.The resulting tools yield insights into the emergence of complex structures in RNA mutational landscapes, structure-function relationships in RNA drug discovery, and the detection of novel conserved interaction networks in large databases of RNA structures.This thesis opens new avenues of research in evolutionary modelling of RNA populations, RNA function prediction, and network data mining"--
