Author: Susan Lehnhardt
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 296

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Book Description

Author: Susan Buchanan
Publisher: Humana
ISBN: 9781493928705
Category : Science
Languages : en
Pages : 0

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Book Description
This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.

Author: Patrick van Gelder
Publisher:
ISBN:
Category :
Languages : en
Pages : 141

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Book Description

Author: Marlies Cornelia Struyvé
Publisher:
ISBN: 9789039300152
Category :
Languages : en
Pages : 95

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Book Description

Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

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Book Description

Author: Weiran Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages : 133

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Book Description
Secretory proteins play critical role in cell survival and pathogenicity and make up approximately 30% of all polypeptides synthesized by gram-negative bacteria. They may be found in a soluble form in the periplasm (the interstitial space between inner and outer membranes), integrated within the outer membrane or excreted outside of the cell in a vesicular or globular form. In all cases, they must first translocate across the inner membrane. A substantial fraction of secretory proteins do so by making use of a cleavable N-terminal extension called signal sequence (or peptide) that is recognized by a dedicated translocation system known as the Sec pathway. Typical Sec-dependent signal sequences have a tripartite structure with a central hydrophobic core and are about 20 residues in length. However, certain secretory proteins implicated in virulence employ Sec-dependent signal peptides that are over 40 residues long. To determine if repetition of canonical, Sec-dependent signal peptides would benefit secretory protein production, we fused the signal sequences of E. carotovora PelB and E. coli OmpA to one another to produce synthetic PelB-OmpA and OmpA-PelB leader peptides. Using periplasmic maltose binding protein (MBP) and outer membrane protein A (OmpA) as model systems, we found that dual signal peptides support Sec-dependent protein translocation and are preferentially cleaved at the signal peptidase I site vicinal to the mature protein. In the case of native OmpA, dual signal peptides increased the requirement for the molecular chaperone Trigger Factor but reduced the accumulation of misfolded precursor and mature species and delayed the acquisition of PBAD promoter mutations that restore cell growth by shutting down recombinant protein synthesis. On the other hand, dual signal sequences did not improve the incorporation of an OmpA1-183-mCherry fusion protein within extracellular outer membrane vesicles (OMVs), as highest yields were obtained with the PelB signal peptide. However, co-expression of periplasmic chaperones (primarily SurA) and components of the outer membrane protein assembly machinery (primarily BamA) improve OMV production by nearly twofold. Overall, our results highlight the potential and limitations of signal sequence and chaperone engineering strategies for the production of secretory proteins and OMVs in E. coli.

Author: P. A. Vanderley
Publisher:
ISBN:
Category :
Languages : en
Pages : 110

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Book Description

Author: Ross Dalbey
Publisher: Elsevier
ISBN: 0080480500
Category : Science
Languages : en
Pages : 441

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Book Description
Protein Targeting, Transport, and Translocation presents an in-depth overview on the topic of protein synthesis, covering all areas of protein science, including protein targeting, secretion, folding, assembly, structure, localization, quality control, degradation, and antigen presentation. Chapters also include sections on the history of the field as well as summary panels for quick reference. Numerous color illustrations complement the presentation of material. This book is an essential reference for anyone in biochemistry and protein science, as well as an excellent textbook for advanced students in these and related fields. - Basic principles and techniques - Targeting adn sorting sequences - Protein export in bacteria - Membrane protein integration into ER and bacterial membranes - Protein translocation across the ER - Disulfide bond formation in prokaryotes and eukaryotes - Quality control in the export pathway - Import of proteins into organelles - The secretory pathway - Vesicular transport - Spectacular color throughout

Author: Gunnar von Heijne
Publisher: R. G. Landes
ISBN:
Category : Science
Languages : en
Pages : 300

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Book Description

Author: Andreas Kuhn
Publisher: Springer
ISBN: 3030187683
Category : Science
Languages : en
Pages : 501

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Book Description
This book provides an up-to-date overview of the architecture and biosynthesis of bacterial and archaeal cell walls, highlighting the evolution-based similarities in, but also the intriguing differences between the cell walls of Gram-negative bacteria, the Firmicutes and Actinobacteria, and the Archaea. The recent major advances in this field, which have brought to light many new structural and functional details, are presented and discussed. Over the past five years, a number of novel systems, e.g. for lipid, porin and lipopolysaccharide biosynthesis have been described. In addition, new structural achievements with periplasmic chaperones have been made, all of which have revealed amazing details on how bacterial cell walls are synthesized. These findings provide an essential basis for future research, e.g. the development of new antibiotics. The book’s content is the logical continuation of Volume 84 of SCBI (on Prokaryotic Cytoskeletons), and sets the stage for upcoming volumes on Protein Complexes.