Stochastic Gene Expression During Lineage Specification of Single T Helper Lymphocytes PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Stochastic Gene Expression During Lineage Specification of Single T Helper Lymphocytes PDF full book. Access full book title Stochastic Gene Expression During Lineage Specification of Single T Helper Lymphocytes by Miaoqing Fang. Download full books in PDF and EPUB format.
Author: Miaoqing Fang
Publisher:
ISBN:
Category :
Languages : en
Pages : 125
Get Book Here
Book Description
The adaptive immune system is an extraordinarily diverse inventory comprised of highly specialized cells, the differentiation of which requires numerous lineage specifications at various developmental stages. The precise control of immune cell differentiation and the delicate balance of their population composition are crucial for effective protection against infectious environmental agents, without triggering autoimmune responses or allergies. It is therefore important to understand at the molecular level in individual cells how lineage commitment is regulated. I explored the heterogeneous gene expression during the lineage specification of single T helper cells, by quantitatively measuring mRNA and protein levels. I have discovered a paradigm of cell lineage specification governed by the signaling interplay between extracellular cues and intracellular transcriptional factors, where the strength of extracellular signaling dominates over the intracellular signaling components. In the presence of extracellular cues, T helper cells stochastically acquire any intermediate Thl/Th2 states. The states of T helper cells can be gradually tuned by depriving availability of extracellular cytokines, which are produced stochastically by a small subpopulation of cells. When extracellular cues are removed, the weak intracellular signaling network reveals its effect, leading to classic mutual exclusion of antagonistic transcriptional factors.
Author: Miaoqing Fang
Publisher:
ISBN:
Category :
Languages : en
Pages : 125
Get Book Here
Book Description
The adaptive immune system is an extraordinarily diverse inventory comprised of highly specialized cells, the differentiation of which requires numerous lineage specifications at various developmental stages. The precise control of immune cell differentiation and the delicate balance of their population composition are crucial for effective protection against infectious environmental agents, without triggering autoimmune responses or allergies. It is therefore important to understand at the molecular level in individual cells how lineage commitment is regulated. I explored the heterogeneous gene expression during the lineage specification of single T helper cells, by quantitatively measuring mRNA and protein levels. I have discovered a paradigm of cell lineage specification governed by the signaling interplay between extracellular cues and intracellular transcriptional factors, where the strength of extracellular signaling dominates over the intracellular signaling components. In the presence of extracellular cues, T helper cells stochastically acquire any intermediate Thl/Th2 states. The states of T helper cells can be gradually tuned by depriving availability of extracellular cytokines, which are produced stochastically by a small subpopulation of cells. When extracellular cues are removed, the weak intracellular signaling network reveals its effect, leading to classic mutual exclusion of antagonistic transcriptional factors.
Author: Jonathan Soboloff
Publisher: CRC Press
ISBN: 149870509X
Category : Medical
Languages : en
Pages : 258
Get Book Here
Book Description
T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.
Author: Rémy Bosselut
Publisher: Humana
ISBN: 9781493928088
Category : Medical
Languages : en
Pages : 0
Get Book Here
Book Description
This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Author: Charles Watson
Publisher: Academic Press
ISBN: 0123694973
Category : Science
Languages : en
Pages : 815
Get Book Here
Book Description
The Mouse Nervous System provides a comprehensive account of the central nervous system of the mouse. The book is aimed at molecular biologists who need a book that introduces them to the anatomy of the mouse brain and spinal cord, but also takes them into the relevant details of development and organization of the area they have chosen to study. The Mouse Nervous System offers a wealth of new information for experienced anatomists who work on mice. The book serves as a valuable resource for researchers and graduate students in neuroscience. Systematic consideration of the anatomy and connections of all regions of the brain and spinal cord by the authors of the most cited rodent brain atlases A major section (12 chapters) on functional systems related to motor control, sensation, and behavioral and emotional states A detailed analysis of gene expression during development of the forebrain by Luis Puelles, the leading researcher in this area Full coverage of the role of gene expression during development and the new field of genetic neuroanatomy using site-specific recombinases Examples of the use of mouse models in the study of neurological illness
Author: Gheorghita Isvoranu
Publisher: BoD – Books on Demand
ISBN: 9535133438
Category : Medical
Languages : en
Pages : 194
Get Book Here
Book Description
This book represents a synergic effort of an international team of specialists in immunology to expand the scientific achievements in the field of lymphocytes. It offers important and specific updated information to researchers, students, teachers, and medical professionals. Moreover, considering the remarkable dynamics of immunology and immunotherapy, this book "Lymphocyte Updates - Cancer, Autoimmunity, and Infection" aims to represent a significant source of concise scientific data and advancement of knowledge in this field. The chapters offer new insights into the latest scientific progress on lymphocyte roles in protective immunity, as well as their involvement in pathogenesis of various disorders.
Author: Isabelle S. Peter
Publisher: Academic Press
ISBN: 0124047467
Category : Science
Languages : en
Pages : 461
Get Book Here
Book Description
Genomic Control Process explores the biological phenomena around genomic regulatory systems that control and shape animal development processes, and which determine the nature of evolutionary processes that affect body plan. Unifying and simplifying the descriptions of development and evolution by focusing on the causality in these processes, it provides a comprehensive method of considering genomic control across diverse biological processes. This book is essential for graduate researchers in genomics, systems biology and molecular biology seeking to understand deep biological processes which regulate the structure of animals during development. Covers a vast area of current biological research to produce a genome oriented regulatory bioscience of animal life Places gene regulation, embryonic and postembryonic development, and evolution of the body plan in a unified conceptual framework Provides the conceptual keys to interpret a broad developmental and evolutionary landscape with precise experimental illustrations drawn from contemporary literature Includes a range of material, from developmental phenomenology to quantitative and logic models, from phylogenetics to the molecular biology of gene regulation, from animal models of all kinds to evidence of every relevant type Demonstrates the causal power of system-level understanding of genomic control process Conceptually organizes a constellation of complex and diverse biological phenomena Investigates fundamental developmental control system logic in diverse circumstances and expresses these in conceptual models Explores mechanistic evolutionary processes, illuminating the evolutionary consequences of developmental control systems as they are encoded in the genome
Author: Jean-Jacques Kupiec
Publisher: World Scientific
ISBN: 981270499X
Category : Science
Languages : en
Pages : 274
Get Book Here
Book Description
In the 17th century, Descartes put forth the metaphor of the machine to explain the functioning of living beings. In the 18th century, La Mettrie extended the metaphor to man. The clock was then used as the paradigm of the machine. In the 20th century, this metaphor still held but the clock was replaced by a computer. Nowadays, the organism is viewed as a robot obeying signals emanating from a computer program controlled by genetic information. This book shows that such a conception leads to contradictions not only in the theory of biology but also in its experimental research program, thereby impeding its development. The analysis of this problem is based on the most recent experimental data obtained in molecular biology as well as the history and philosophy of biology. It shows that the machine theory did not succeed in breaking with Aristotle's finalism. The book presents a new approach to biological systems based on cellular Darwinism. Genes are ruled by probabilistic mechanisms allowing cells to differentiate stochastically. Embryo development is not governed by a determinist genetic program but by natural selection occurring among cell populations inside the organism. This theory has considerable philosophical consequences. Man may be a machine but he is a random one.
Author: Eran Meshorer
Publisher: Springer Science & Business Media
ISBN: 1441970371
Category : Science
Languages : en
Pages : 246
Get Book Here
Book Description
Stem cells have been gaining a lot of attention in recent years. Their unique potential to self-renew and differentiate has turned them into an attractive model for the study of basic biological questions such as cell division, replication, transcription, cell fate decisions, and more. With embryonic stem (ES) cells that can generate each cell type in the mammalian body and adult stem cells that are able to give rise to the cells within a given lineage, basic questions at different developmental stages can be addressed. Importantly, both adult and embryonic stem cells provide an excellent tool for cell therapy, making stem cell research ever more pertinent to regenerative medicine. As the title The Cell Biology of Stem Cells suggests, our book deals with multiple aspects of stem cell biology, ranging from their basic molecular characteristics to the in vivo stem cell trafficking of adult stem cells and the adult stem-cell niche, and ends with a visit to regeneration and cell fate reprogramming. In the first chapter, “Early embryonic cell fate decisions in the mouse”, Amy Ralson and Yojiro Yamanaka describe the mechanisms that support early developmental decisions in the mouse pre-implantation embryo and the current understanding of the source of the most immature stem cell types, which includes ES cells, trophoblast stem (TS) cells and extraembryonic endoderm stem (XEN) cells.
Author: Shirley A. Bayer
Publisher: CRC Press
ISBN: 0203507487
Category : Medical
Languages : en
Pages : 378
Get Book Here
Book Description
The brain in the second trimester, the subject of Volume 3, is nearing anatomical maturity throughout the brainstem. In contrast, the neurogenesis and neuronal migration are still in progress in the cerebral cortex and cerebellum. Consequently, the authors chose to focus on the migration, sojourning, and settling of the neuronal populations belo
Author: Andreas Radbruch
Publisher: Springer Science & Business Media
ISBN: 3662041294
Category : Science
Languages : en
Pages : 365
Get Book Here
Book Description
The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
