Single Molecule Microscopy in Neurobiology PDF Download
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Author: Nobuhiko Yamamoto
Publisher: Humana
ISBN: 9781071605318
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.
Author: Nobuhiko Yamamoto
Publisher: Humana
ISBN: 9781071605318
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume looks at the methodology and techniques used by experts to study how certain molecules function in specific locations, and their temporal patterns. Chapters in this book cover topics such as in vivo single-molecule tracking of voltage-gated calcium channels with split-fluorescent proteins in CRISPR-engineering C. elegans; protein-protein interactions in membranes using single particle tracking; neuropathological diseases revealed by quantum-dot single particle tracking; SPoD-OnSPAN; and investigating molecular diffusion inside small neuronal compartments with two-photon fluorescence correlation spectroscopy. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Single Molecule Microscopy is a valuable resource for any researcher interested in learning more about this important field.
Author: Floris G. Wouterlood
Publisher: Academic Press
ISBN: 0123858720
Category : Medical
Languages : en
Pages : 298
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Book Description
In the biomedical sciences, the confocal laser scanning microscope (CLSM) has become the instrument of choice for producing high-resolution images and 3D reconstruction, breaking the barriers of conventional optical microscopy. Wouterlood (anatomy, VU U. Medical Center, Amsterdam, the Netherlands) introduces the confocal principle which eliminates out-of-focus haze, its components, and relevant equations. International scientists explain the principles and related methods of stimulated emission depletion (SRED), single molecule localization, and coherent anti-Stokes Raman (CARS) microscopy; labeling approaches; preparation of samples for imaging; and applications of, and developments in, this new wave of imaging, e.g., visualization of neuronal networks, DNA, and myelin. The text includes color and b&w images, and referral to an online CLSM simulator. Academic Press is an imprint of Elsevier. Annotation ©2013 Book News, Inc., Portland, OR (booknews.com).
Author: Rafael Yuste
Publisher: CSHL Press
ISBN: 0879696923
Category : Medical
Languages : en
Pages : 813
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Book Description
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
Author: J. Chad
Publisher: Oxford University Press
ISBN: 9780199632541
Category : Medical
Languages : en
Pages : 568
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Book Description
Neurobiology consists of the study of processes across a spectrum of size scales from whole animal to single molecule, and the structure of these two companion volumes reflects this. Cellular Neurobiology covers techniques from basic in vitro maintenance of cells, through perfusion and recording methods, to advanced topics such as optical imaging of ionic activity and mathematical modelling of the properties of excitable membranes. Molecular Neurobiology is principally concerned with understanding the functions of molecules and the resolution of processes at the molecular level. Sections cover characterization and recording of the properties of ionic channels, section messenger systems and molecular biological approaches. The variety of techniques covered will appeal to both the new and the established researcher. Each chapter gives information on basic methods and direct practical assistance in designing and undertaking new experiments.
Author: P. Michael Conn
Publisher: Academic Press
ISBN: 1483268179
Category : Nature
Languages : en
Pages : 511
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Book Description
Methods in Neurosciences, Volume 3: Quantitative and Qualitative Microscopy is a collection of papers that deals with microscopic techniques in statistical measures. This volume describes microscopy using sophisticated stains and dyes to advance observation of tests and experiments. Section I describes autoradiography including micro chemical methods, high-resolution autoradiography, and single- or double-label quantitative autoradiography for use in imaging of brain activity patterns or determining cerebral physiology. Section II discusses the quantification of structures through statistical and computational methods including dynamic video imaging technology. Section III explains the use of tracers, toxins, or dyes in tracing neuronal connections. One paper addresses the use of small injections of axonally transported fluorescent tracers. Section IV explains staining technology such as using the silver impregnation method for frozen sections of human nervous tissue that are gathered from tissues preserved in formalin. Section V addresses freezing techniques and those using freeze-fracture methods in neurobiology. The text also discusses cryoprotection and other freezing methods to control ice crystals found in fixed or unfixed brain tissues. Section VI presents the combined and high-resolution methods in polarization microscopy and microscopic investigations. Cellular biologists, micro-chemists, and scientific researchers in the field of micro- and cellular biology will appreciate this book.
Author:
Publisher: Elsevier
ISBN: 008049658X
Category : Science
Languages : en
Pages : 521
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Book Description
This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. It is an essential guide for students and scientists in cell biology, neuroscience, and many other areas of biological and biomedical research, as well as research directors and technical staff of microscopy and imaging facilities. An integrated and up-to-date coverage on the many various techniques and uses of the confocal microscope (CM). Includes detailed protocols accessible to new users Details how to set up and run a "Confocal Microscope Core Facility" Contains over 170 figures
Author: Constance Hammond
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 496
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Book Description
This new advanced level textbook introduces a novel way to learn and teach in the neurosciences. Features include: a building-block approach beginning with a clear and functional explanation of the molecular aspects of ion channels; an evaluation of the properties of excitable and secretory cells, compiled by summing up the different ion channels; an approach based on the examination of classic experiments, which avoids dogmatic statements and facilitates the understanding of more complex neuronal behaviour; over 400 illustrations to aid comprehension of the topics presented; and appendices that provide explanations of neurobiological techniques. This book helps students and teachers alike to view neurobiology in a molecular, cellular, and above all, experimental way which makes learning easier and more enjoyable.
Author: Haruyuki Kamiya
Publisher: Frontiers Media SA
ISBN: 2889661873
Category : Science
Languages : en
Pages : 272
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Book Description
This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.
Author: U. Valentin Nägerl
Publisher: Humana
ISBN: 9781493949182
Category : Medical
Languages : en
Pages : 0
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Book Description
Synapses underlie rapid and flexible neural communication in the brain and they hold the key to understanding higher brain functions in health and disease. Because they are very small and highly dynamic, it is very difficult to study them with traditional techniques. Fortunately, recent ground-breaking advances in optical microscopy (e.g. STED, PALM, STORM, SIM) have greatly improved our ability to image living synapses at the nanoscale, even down to the level of single molecules. The proposed volume brings together leading researchers to review these exciting new techniques and their application in neurobiological research. It will explain and discuss the basic principles behind the various superresolution modalities, how they are implemented, what their scope and limitations are etc. In addition, several key research discoveries on synapses enabled by these novel approaches will be highlighted.
Author: Fritjof Helmchen
Publisher:
ISBN: 9780879699376
Category : Brain
Languages : en
Pages : 0
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Book Description
As imaging technologies have revolutionized research in many areas of biology and medicine, neuroscientists have often pioneered the use of these new visualization techniques. This volume is an essential guide to discovering and implementing these techniques in the neuroscience lab.
