Self-assembling Proteins as Templates for Gold Nanoparticle Arrays PDF Download
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Author: Amish Ashwin Patel
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
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Book Description
Author: Amish Ashwin Patel
Publisher:
ISBN:
Category :
Languages : en
Pages : 286
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Book Description
Author: Kyle Webster
Publisher:
ISBN:
Category : Nanotechnology
Languages : en
Pages : 220
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Book Description
Natural systems self-assemble from the nanoscale up through biomolecules, such as proteins that exhibit high functional complexity. The capabilities of proteins to control structure at the nanoscale exceed what we can achieve with top down manufacturing techniques and so can be leveraged for use as scaffolds in nanotechnology. By functionalising proteins with artificial nanostructures, we can go beyond natural biochemistry in developing new hybrid bionanotechnologies. Building on previous work, development of human peroxiredoxin three (HsPrx3) as a generalisable nanoparticle assembly system was continued. HsPrx3 was adapted through targeted mutation and protein engineering to enable functionalisation with a variety of nanoparticle cargos. HsPrx3 variants were characterised by size exclusion chromatography (SEC), transmission electron microscopy (TEM), analytical ultracentrifugation (AUC), and an X-ray crystallographic structure (Chapter 3) and tested in their capacity for unctionalisation with nanoparticle cargo. This entailed functionalisation and characterisation of HsPrx3 – gold nanoparticle conjugates followed by polyoxometelate functionalisation using a Mo132 structure (Chapter 4). Characterisation of functionalised proteins was undertaken using multiwave length SEC, spectroscopy, and energy dispersive X-ray spectroscopy (EDX). Building on previous work, demonstrating the capacity for peptide structures to template higher order HsPrx3 tube and array structures, protocols were developed for use with functionalised HsPrx3 (Chapter 5). Characterisation of HsPrx3 – peptide interactions on a SiO2 surface required combined quartz crystal microbalance with dissipation (QCM-D) and atomic force microscopy (AFM) to develop an understanding the conditions of array assembly and interactions of HsPrx3 and peptide structures with each other and a surface (Chapter 6). This work expands the array of protein scaffolds available to future researchers through design, production and testing of new HsPrx3 variants, a new X-ray crystallography structure that challenges existing understanding of the stabilising mechanism of the S78C mutation. Additionally, this research features a new HsPrx3 packing pattern and demonstrates the functionalisation of a self-assembling protein scaffold with a polyoxometalate. Finally, this is the first example of imaging and characterisation of Mo132 by TEM and EDX, verification of the protein templating capacity of two self-assembling peptide nanostructures, and the development of a novel method for validating the agreement of QCM-D and AFM data through density estimates.
Author: Alok Pandya
Publisher: Academic Press
ISBN: 032390985X
Category : Medical
Languages : en
Pages : 316
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Book Description
Design, Principle and Application of Self-Assembled Nanobiomaterials in Biology and Medicine discusses recent advances in science and technology using nanoscale units that show the novel concept of combining nanotechnology with various research disciplines within both the biomedical and medicine fields. Self-assembly of molecules, macromolecules, and polymers is a fascinating strategy for the construction of various desired nanofabrication in chemistry, biology, and medicine for advanced applications. It has a number of advantages: (1) It is involving atomic-level modification of molecular structure using bond formation advanced techniques of synthetic chemistry. (2) It draws from the enormous wealth of examples in biology for the development of complex, functional structures. (3) It can incorporate biological structures directly as components in the final systems. (4) It requires that the target self-assembled structures be thermodynamically most stable with relatively defect-free and self-healing. In this book, we cover the various emerging self-assembled nanostructured objects including molecular machines, nano-cars molecular rotors, nanoparticles, nanosheets, nanotubes, nanowires, nano-flakes, nano-cubes, nano-disks, nanorings, DNA origami, transmembrane channels, and vesicles. These self-assembled materials are used for sensing, drug delivery, molecular recognition, tissue engineering energy generation, and molecular tuning. - Provides a basic understanding of how to design, and implement various self-assembled nanobiomaterials - Covers principles implemented in the constructions of novel nanostructured materials - Offers many applications of self-assemblies in fluorescent biological labels, drug and gene delivery, bio-detection of pathogens, detection of proteins, probing of DNA structure, tissue engineering, and many more
Author: Jennifer J. McManus
Publisher: Humana
ISBN: 9781493996803
Category : Science
Languages : en
Pages : 266
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Book Description
This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Author: Matthew M. Shindel
Publisher:
ISBN:
Category :
Languages : en
Pages : 316
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Book Description
Author: Stefan Howorka
Publisher: Academic Press
ISBN: 0124159060
Category : Medical
Languages : en
Pages : 427
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Book Description
This volume explores some of the most exciting recent advances in basic research on molecular assembly in natural and engineered systems and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and idea Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields
Author: Kathy Lu
Publisher: John Wiley & Sons
ISBN: 1118408950
Category : Technology & Engineering
Languages : en
Pages : 379
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Book Description
Serving as the only systematic and comprehensive treatment on the topic of nanoparticle-based materials, this book covers synthesis, characterization, assembly, shaping and sintering of all types of nanoparticles including metals, ceramics, and semiconductors. A single-authored work, it is suitable as a graduate-level text in nanomaterials courses.
Author: Simon Robert Hall
Publisher: World Scientific
ISBN: 1908978392
Category : Science
Languages : en
Pages : 216
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Book Description
In terms of structural complexity, the natural world presents innumerable examples of stunning beauty and high functionality, usually with the minimum of material and energy expenditure. Materials chemists can harness these amazing structures as ready-made scaffolds on which to grow inorganic phases which replicate the underlying complexity, thereby producing materials with greatly enhanced physical properties. This book comprehensively describes the entire range of natural materials that have been used in this way and the inorganic phases which result from them. The book covers simple molecules such as cellulose and chitin, to large biological constructs such as bacterial proteins, viruses and pollen. Practically every inorganic material has been synthesized using biotemplating methods and the book reflects this, ranging from simple oxides and carbonates such as silica and calcite, to complex semi- and superconducting materials. The book also discusses the formation of these materials from a mechanistic point of view, thereby enabling the reader to better understand the processes involved in biotemplated mineralization./a
Author: Omar Azzaroni
Publisher: Royal Society of Chemistry
ISBN: 1788018028
Category : Science
Languages : en
Pages : 521
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Book Description
This book covers introductory features underlying the field of nanoarchitectonices, presenting a unifying overview of the theoretical aspects and emerging applications that are changing the capability to understand and design advanced functional materials.
Author:
Publisher: Academic Press
ISBN: 0123751772
Category : Technology & Engineering
Languages : en
Pages : 307
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Book Description
Many of the devices and systems used in modern industry are becoming progressively smaller and have reached the nanoscale domain. Nanofabrication aims at building nanoscale structures, which can act as components, devices, or systems, in large quantities at potentially low cost. Nanofabrication is vital to all nanotechnology fields, especially for the realization of nanotechnology that involves the traditional areas across engineering and science. - Includes chapters covering the most important Nanofabrication techniques, which aids comprehensive understanding of the latest manufacturing technologies encountered in the field of nano-level manufacturing which is essential for preparing for advanced study and application in nanofabrication techniques by enabling thorough understanding of the entire nanofabrication process as it applies to advanced electronic and related manufacturing technologies - Each chapter covers a nanofabrication technique comprehensively, which allows the reader to learn to produce nanometer-level products as well as collect, process, and analyze data, improve process parameters, and how to assist engineers in research, development and manufacture of the same - Includes contributions from recognized experts from around the globe, making the reader aware of variations in similar techniques applied in different geographical locations and is better positioned to establish all possible global applications
