The Nucleic Acid Protocols Handbook

The Nucleic Acid Protocols Handbook PDF Author: Ralph Rapley
Publisher: Springer Science & Business Media
ISBN: 1592590381
Category : Science
Languages : en
Pages : 997

Get Book Here

Book Description
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

The Nucleic Acid Protocols Handbook

The Nucleic Acid Protocols Handbook PDF Author: Ralph Rapley
Publisher: Springer Science & Business Media
ISBN: 1592590381
Category : Science
Languages : en
Pages : 997

Get Book Here

Book Description
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

Prokaryotic Gene Regulation

Prokaryotic Gene Regulation PDF Author: Eveline Peeters
Publisher: Springer Nature
ISBN: 107162413X
Category : Science
Languages : en
Pages : 321

Get Book Here

Book Description
This volume presents a collection of versatile methodologies to investigate prokaryotic gene regulation, with focus on the different levels of information processing and usefulness for various model organisms, whether archaeal, bacterial, or both. The chapters in this book are divided into four sections. Section One covers methods that enable the study of the structure of the bacterial/archaeal chromosome, the main template for all gene regulatory processes, and its epigenetic modification. Section Two looks at a selection of approaches that enable higher levels of understanding of transcription initiation, a key step in information processing. Section Three discusses the investigation of regulating transcription factors, which are often considered the main players in gene regulation in prokaryotic cells. The Fourth Section focuses on the next stage of information processing at which gene regulation occurs, namely the RNA-based level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Prokaryotic Gene Regulation: Methods and Protocols is a valuable resource for researchers interested in learning more about this diverse field.

Renal Cancer

Renal Cancer PDF Author: Jack H. Mydlo
Publisher: Springer Science & Business Media
ISBN: 1592591442
Category : Medical
Languages : en
Pages : 397

Get Book Here

Book Description
The purpose of Renal Cancer: Methods and Protocols is to introduce the surgeon, clinician, investigator, and research scientist to the basic methods employed in the diagnosis and treatment of renal cancer. Treatment of localized renal cancer is surgical. Treatment of metastasis with resection, radiotherapy, chemotherapy, and immunotherapy has had limited results. Therefore, new avenues of treatment are necessary. In planning this work, I have attempted to incorporate coverage by specialists from a variety of disciplines, each applying their individual expertise in renal cancer therapy. Though many of the participating authors are urologists, there are also valuable contributions from medical oncologists, laboratory inv- tigators, and pathologists. One of the inherent problems in publishing a book that describes molecular techniques, especially those techniques that are rapidly evolving, is that some of these may become obsolete after a few years. However, many of the procedures detailed here are standard approaches that have already wi- stood the test of time. Reverse transcriptase PCR, or RT-PCR, a technique that produces m- tiple copies of selected sequences of DNA, has a sensitivity of a millionfold amplication of a single cell, and thus constitutes a very precise technique for measuring the presence of tumor cells. By combining this technique with genomic hybridization, cadherin,ß and metalloproteinase expression we may then reveal other important factors in the detection, staging, aggressiveness, and treatment of this disease.

Coral reef research methods

Coral reef research methods PDF Author: Shashank Keshavmurthy
Publisher: Frontiers Media SA
ISBN: 283250888X
Category : Science
Languages : en
Pages : 256

Get Book Here

Book Description


Manual of Clinical Microbiology, 4 Volume Set

Manual of Clinical Microbiology, 4 Volume Set PDF Author: Karen C. Carroll
Publisher: John Wiley & Sons
ISBN: 1683674812
Category : Medical
Languages : en
Pages : 6886

Get Book Here

Book Description
Revised by a collaborative, international, interdisciplinary team of editors and authors, this edition of the Manual of Clinical Microbiology includes the latest applications of genomics and proteomics and is filled with current findings regarding infectious agents, leading-edge diagnostic methods, laboratory practices, and safety guidelines. This edition also features four new chapters: Diagnostic Stewardship in Clinical Microbiology; Salmonella; Escherichia and Shigella; and Morganellaceae, Erwiniaceae, Hafniaceae, and Selected Enterobacterales. This seminal reference of microbiology continues to set the standard for state-of-the-science laboratory practice as the most authoritative reference in the field of microbiology. If you are looking for online access to the latest from this reference or site access for your lab, please visit www.wiley.com/learn/clinmicronow.

Circular RNAs

Circular RNAs PDF Author: Christoph Dieterich
Publisher: Springer Nature
ISBN: 1071636782
Category :
Languages : en
Pages : 326

Get Book Here

Book Description


Emerging Infectious Diseases

Emerging Infectious Diseases PDF Author:
Publisher:
ISBN:
Category : Communicable diseases
Languages : en
Pages : 1374

Get Book Here

Book Description


Cancer Drug Resistance

Cancer Drug Resistance PDF Author: Marta Baiocchi
Publisher: Springer Nature
ISBN: 1071625136
Category : Medical
Languages : en
Pages : 247

Get Book Here

Book Description
This volume discusses the latest techniques used to identify cancer drug resistance determinants at the molecular, cellular, and functional levels. Chapters in this book cover up-to-date topics including tumor-microenvironment cell co-culture methods and microfluidics systems; workflows for functional assessment of drug resistance in vitro and in vivo; quantitative techniques for identifying quiescent blood-flow circulating cells; and single-cell characterization methods, such as mass cytometry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Cancer Drug Resistance: Methods and Protocols is a valuable resource for all scientists and researchers who are looking to learn more about the latest developments in understanding and overcoming anticancer drug resistance.

Journal of the National Cancer Institute

Journal of the National Cancer Institute PDF Author:
Publisher:
ISBN:
Category : Cancer
Languages : en
Pages : 516

Get Book Here

Book Description


Bacteria and Fungi from Fish and other Aquatic Animals, 2nd Edition

Bacteria and Fungi from Fish and other Aquatic Animals, 2nd Edition PDF Author: Nicky B Buller
Publisher: CABI
ISBN: 1845938054
Category : Science
Languages : en
Pages : 921

Get Book Here

Book Description
This practical book provides an updated resource for the identification of bacteria found in animals inhabiting the aquatic environment, illustrated with colour photos. It contains expanded biochemical identification tables to include newly identified pathogenic and saprophytic bacteria, molecular identification tests now available for a greater number of aquatic bacterial pathogens, more information on the pathogenesis and virulence of each organism and new coverage of traditional and molecular identification of fungal pathogens and quality assurance standards for laboratories.