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Author: Andrés Aguilera
Publisher: Springer Nature
ISBN: 1071624776
Category : Science
Languages : en
Pages : 468
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Book Description
This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids in vitro and in vivo. Several protocols permit studying non-canonical RNA nucleotides in the R-loop context, as well as a number of specific protocols devoted to the investigation of R-loop topology and their functional roles in the biology of mitochondria and telomeres. Finally, a large block of chapters is dedicated to different methods allowing genome-wide mapping of DNA-RNA hybrids in various organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, R-Loops: Methods and Protocols serves as an ideal resource for those working on R-loop homeostasis but also to scientists studying such areas of molecular and cell biology as genome integrity, DNA replication and repair, chromatin remodeling, transcription, RNA processing, modification and export, as well as for researchers elucidating the molecular mechanisms of cancer and genetic diseases.
Author: Andrés Aguilera
Publisher: Springer Nature
ISBN: 1071624776
Category : Science
Languages : en
Pages : 468
Get Book Here
Book Description
This detailed book compiles a series of laboratory protocols covering the most important aspects of R-loop biology. Beginning with a range of methods allowing for the detection of DNA-RNA hybrids, as well as their purification and visualization by electron microscopy, the volume continues with methods based on the use of RNase H-derived tools to detect DNA-RNA hybrids in vitro and in vivo. Several protocols permit studying non-canonical RNA nucleotides in the R-loop context, as well as a number of specific protocols devoted to the investigation of R-loop topology and their functional roles in the biology of mitochondria and telomeres. Finally, a large block of chapters is dedicated to different methods allowing genome-wide mapping of DNA-RNA hybrids in various organisms. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, R-Loops: Methods and Protocols serves as an ideal resource for those working on R-loop homeostasis but also to scientists studying such areas of molecular and cell biology as genome integrity, DNA replication and repair, chromatin remodeling, transcription, RNA processing, modification and export, as well as for researchers elucidating the molecular mechanisms of cancer and genetic diseases.
Author: Hadley Wickham
Publisher: CRC Press
ISBN: 1498759807
Category : Mathematics
Languages : en
Pages : 669
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Book Description
An Essential Reference for Intermediate and Advanced R Programmers Advanced R presents useful tools and techniques for attacking many types of R programming problems, helping you avoid mistakes and dead ends. With more than ten years of experience programming in R, the author illustrates the elegance, beauty, and flexibility at the heart of R. The book develops the necessary skills to produce quality code that can be used in a variety of circumstances. You will learn: The fundamentals of R, including standard data types and functions Functional programming as a useful framework for solving wide classes of problems The positives and negatives of metaprogramming How to write fast, memory-efficient code This book not only helps current R users become R programmers but also shows existing programmers what’s special about R. Intermediate R programmers can dive deeper into R and learn new strategies for solving diverse problems while programmers from other languages can learn the details of R and understand why R works the way it does.
Author: Hadley Wickham
Publisher: "O'Reilly Media, Inc."
ISBN: 1491910364
Category : Computers
Languages : en
Pages : 521
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Book Description
Learn how to use R to turn raw data into insight, knowledge, and understanding. This book introduces you to R, RStudio, and the tidyverse, a collection of R packages designed to work together to make data science fast, fluent, and fun. Suitable for readers with no previous programming experience, R for Data Science is designed to get you doing data science as quickly as possible. Authors Hadley Wickham and Garrett Grolemund guide you through the steps of importing, wrangling, exploring, and modeling your data and communicating the results. You'll get a complete, big-picture understanding of the data science cycle, along with basic tools you need to manage the details. Each section of the book is paired with exercises to help you practice what you've learned along the way. You'll learn how to: Wrangle—transform your datasets into a form convenient for analysis Program—learn powerful R tools for solving data problems with greater clarity and ease Explore—examine your data, generate hypotheses, and quickly test them Model—provide a low-dimensional summary that captures true "signals" in your dataset Communicate—learn R Markdown for integrating prose, code, and results
Author: Arthur Kornberg
Publisher: University Science Books
ISBN: 9781891389443
Category : Science
Languages : en
Pages : 964
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Book Description
DNA Replication, second edition, a classic of modernscience, is now back in print in a paperback edition. Kornberg and Baker'sinsightful coverage of DNA replication and related cellular processes have madethis the standard reference in the field.
Author: Douglas R. Hofstadter
Publisher: Basic Books (AZ)
ISBN: 0465030785
Category : Philosophy
Languages : en
Pages : 544
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Book Description
Argues that the key to understanding ourselves and consciousness is the "strange loop," a special kind of abstract feedback loop that inhabits the brain.
Author: Xiaogang An
Publisher: Infinite Study
ISBN:
Category : Mathematics
Languages : en
Pages : 10
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Book Description
Abel-Grassmann’s groupoid and neutrosophic extended triplet loop are two important algebraic structures that describe two kinds of generalized symmetries. In this paper, we investigate quasi AG-neutrosophic extended triplet loop, which is a fusion structure of the two kinds of algebraic structures mentioned above.
Author: Kishor K. Bhakat
Publisher: Springer Nature
ISBN: 1071633732
Category : Science
Languages : en
Pages : 267
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Book Description
This detailed volume provides a comprehensive set of experimental protocols and useful strategies to examine the repair of damaged bases via the Base Excision Repair (BER) pathway in vitro and in cells. Beginning with multiple molecular and cellular techniques to examine the excision of damaged bases from double-stranded DNA or DNA wrapped in a nucleosome, the book continues with sections covering procedures to detect and quantify the damaged bases, protein DNA crosslinks, and double-strand breaks, experimental procedures to identify DNA repair protein interactome by conventional tandem affinity purification followed by mass spectroscopy analysis, as well as the analysis of genome-wide binding of DNA repair proteins and copy number variations of the DNA damage response gene in tumors. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Excision Repair Pathway: Methods and Protocols serves as a valuable resource for novices and experts trying to examine the repair of various types of DNA lesions in vitro and in cell by the distinct set of proteins in the BER pathway.
Author:
Publisher: Elsevier
ISBN: 008046467X
Category : Science
Languages : en
Pages : 613
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Book Description
This volume emphasizes the intracellular consequences of DNA damage, describing procedures for analysis of checkpoint responses, DNA repair in vivo, replication fork encounter of DNA damage, as well as biological methods for analysis of mutation production and chromosome rearrangements. It also describes molecular methods for analysis of a number of genome maintenance activities including DNA ligases, helicases, and single-strand binding proteins.*Part B of a 2-part series*Addresses DNA maintenance enzymes*Discusses damage signaling*Presents In vivo analysis of DNA repair*Covers mutation and chromosome rearrangements
Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
ISBN: 1803556218
Category : Medical
Languages : en
Pages : 214
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Book Description
Gene expression is dependent on multiple steps, including transcription, RNA processing, and translation. Importantly, recent studies revealed that gene expression is regulated by chromatin structures and non-coding RNA profiles. Elucidating the molecular mechanisms may contribute to the development of novel therapeutics for aging-related diseases, including cancer and neurodegenerative diseases. This book provides a comprehensive overview of gene expression and its role in human disease. It consists of nine chapters organized into two sections on molecular mechanisms in controlling gene expression and the relationships between transcriptional control and human disease.
Author: William E. Paul
Publisher: Lippincott Williams & Wilkins
ISBN: 9780781765190
Category : Medical
Languages : en
Pages : 1658
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Book Description
Now thoroughly revised and updated, this comprehensive, up-to-date text is ideal for graduate students, post-doctoral fellows, microbiologists, infectious disease physicians, and any physician who treats diseases in which immunologic mechanisms play a role.
