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Author: Nicholas F. Parrish
Publisher: Springer Nature
ISBN: 107162380X
Category : Science
Languages : en
Pages : 398
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Book Description
This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.
Author: Nicholas F. Parrish
Publisher: Springer Nature
ISBN: 107162380X
Category : Science
Languages : en
Pages : 398
Get Book Here
Book Description
This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, piRNA: Methods and Protocols serves as an ideal guide for researchers seeking to elucidate the numerous mysteries of this area of multicellular biology.
Author: John Eddison
Publisher: CRC Press
ISBN: 9781584880332
Category : Mathematics
Languages : en
Pages : 476
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Book Description
Until recently, acquiring a background in the basic methodological principles that apply to most types of investigations meant struggling to obtain results through laborious calculations. The advent of statistical software packages has removed much of the tedium and many of the errors of manual calculations and allowed a marked increase in the depth and sophistication of analyses. Although most statistics classes now incorporate some instruction in using a statistics package, most introductory texts do not. Quantitative Investigations in the Biosciences using MINITAB fills this void by providing an introduction to investigative methods that, in addition to outlining statistical principles and describing methods of calculations, also presents essential commands and interprets output from the statistics package MINITAB. The author introduces the three basic elements of investigations-design, analysis, and reporting-using an extremely accessible approach that keeps mathematical detail to a minimum. He groups statistical tests according to the type of problem they are used to examine, such as comparisons, sequential relationships, and associations. Quantitative Investigations in the Biosciences using MINITAB draws techniques and examples from a variety of subjects, ranging from physiology and biochemistry through to ecology, behavioral sciences, medicine, agriculture and horticulture, and complements the mathematical results with formal conclusions for all of the worked examples. It thus provides an ideal handbook for anyone in virtually any field who wants to apply statistical techniques to their investigations.
Author: Naoki Tanimizu
Publisher: Springer Nature
ISBN: 1071625578
Category : Science
Languages : en
Pages : 283
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Book Description
This volume details experimental protocols to study structure and function of hepatocytes in vivo and in vitro, and for characterizing heterogeneity and plasticity of hepatocytes in healthy and in injured liver. Chapters guide readers through separating hepatocyte subfractions, characterizing the heterogeneity, novel culture systems, hepatocyte-like cells from human pluripotent stem cells, analyzing drug metabolism, monitoring protein sorting, manipulating gene expression, and methods for expanding primary hepatoblastoma and identifying hepatocellular carcinoma subtypes. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Hepatocytes: Methods and Protocols aims to provide techniques to further understand molecular mechanisms involved in the regulating hepatocyte functions and underlying liver diseases.
Author: Izuho Hatada
Publisher: Springer Nature
ISBN: 1071627244
Category : Science
Languages : en
Pages : 292
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Book Description
This detailed volume provides a comprehensive collection of protocols for epigenomic research, powering our ability to analyze epigenetic modifications across the entire genome. Beginning with methods used to investigate epigenomic modifications such as DNA methylation, histone modifications, and chromatin structure, the book continues with methods for manipulating the epigenome, including platforms for epigenome editing, inducible systems for epigenome editing, and epigenetically modified animals. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Epigenomics: Methods and Protocols serves as an ideal resource for researchers looking to further expand the utility and scope of epigenomics research.
Author: M Jaffe
Publisher: Elsevier
ISBN: 0857093681
Category : Technology & Engineering
Languages : en
Pages : 337
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Book Description
Biomaterials and medical devices must be rigorously tested in the laboratory before they can be implanted. Testing requires the right analytical techniques. Characterization of biomaterials reviews the latest methods for analyzing the structure, properties and behaviour of biomaterials.Beginning with an introduction to microscopy techniques for analyzing the phase nature and morphology of biomaterials, Characterization of biomaterials goes on to discuss scattering techniques for structural analysis, quantitative assays for measuring cell adhesion, motility and differentiation, and the evaluation of cell infiltration and tissue formation using bioreactors. Further topics considered include studying molecular-scale protein-surface interactions in biomaterials, analysis of the cellular genome and abnormalities, and the use of microarrays to measure cellular changes induced by biomaterials. Finally, the book concludes by outlining standards and methods for assessing the safety and biocompatibility of biomaterials.With its distinguished editors and international team of expert contributors, Characterization of biomaterials is an authoritative reference tool for all those involved in the development, production and application of biomaterials. - Reviews the latest methods for analyzing the structure, properties and behaviour of biomaterials - Discusses scattering techniques for structural analysis, quantitative assays for measuring cell adhesion, and motility and differentiation - Examines the evaluation of cell infiltration and tissue formation using bioreactors
Author: Lilyana Pramatarova
Publisher: BoD – Books on Demand
ISBN: 9533072717
Category : Medical
Languages : en
Pages : 658
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Book Description
Bio-mimicry is fundamental idea "How to mimic the Nature" by various methodologies as well as new ideas or suggestions on the creation of novel materials and functions. This book comprises seven sections on various perspectives of bio-mimicry in our life; Section 1 gives an overview of modeling of biomimetic materials; Section 2 presents a processing and design of biomaterials; Section 3 presents various aspects of design and application of biomimetic polymers and composites are discussed; Section 4 presents a general characterization of biomaterials; Section 5 proposes new examples for biomimetic systems; Section 6 summarizes chapters, concerning cells behavior through mimicry; Section 7 presents various applications of biomimetic materials are presented. Aimed at physicists, chemists and biologists interested in biomineralization, biochemistry, kinetics, solution chemistry. This book is also relevant to engineers and doctors interested in research and construction of biomimetic systems.
Author: Haiwei Luo
Publisher: Springer Nature
ISBN: 1071626914
Category : Science
Languages : en
Pages : 365
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Book Description
This volume explores the latest techniques used to study environmental microbial evolution, with a focus on methods capable of addressing deep evolution at long timescales. The chapters in this book are organized into three parts. Part One introduces molecular dating approaches and time calibration ideas that allow for the determination of evolutionary timescales of microbial lineages. Part Two describes several advanced phylogenomic tools such as models for genome tree construction, a taxon sampling method, outgroup-independent tree-rooting methods, and gene family evolution models. Part Three covers techniques used to study trait evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Environmental Microbial Evolution: Methods and Protocols is a valuable tool for all researchers who are interested in learning more about this important and evolving field.
Author: Eckhard Jankowsky
Publisher: Academic Press
ISBN: 0123965462
Category : Medical
Languages : en
Pages : 590
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Book Description
This work integrates the current knowledge about RNA helicases from diverse fields ranging from cell and developmental biology to mechanistic enzymology and structural biology into one useful resource.
Author: Eisuke Gotoh
Publisher: Springer Nature
ISBN: 1071624334
Category : Science
Languages : en
Pages : 195
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Book Description
This volume provides essential and fundamental protocols on manipulation chromosome. Chapters details methods on the preparation of mitotic chromosome, chromosome aberration, micronucleus (MN), comet assay, karyotyping, Fluorescent in situ hybridization (FISH), premature chromosome condensation (PCC), immunohistochemistry (IHC) staining, new generation sequencing technology and new chromosome concepts, such as epigenetic and its cause of cancer are presented. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Chromosome Analysis: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.
Author:
Publisher: Academic Press
ISBN: 0080950965
Category : Medical
Languages : en
Pages : 389
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Book Description
First published in 1963, Advances in Parasitology contains comprehensive and up-to-date reviews in all areas of interest in contemporary parasitology. Advances in Parasitology includes medical studies on parasites of major influence, such as Plasmodium falciparum and trypanosomes. The series also contains reviews of more traditional areas, such as zoology, taxonomy, and life history, which shape current thinking and applications. Eclectic volumes are supplemented by thematic volumes on various topics, including control of human parasitic diseases and global mapping of infectious diseases. - Informs and updates on all the latest developments in the field - Contributions from leading authorities and industry experts
