Purifying Proteins for Proteomics PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Purifying Proteins for Proteomics PDF full book. Access full book title Purifying Proteins for Proteomics by Richard J. Simpson. Download full books in PDF and EPUB format.
Author: Richard J. Simpson
Publisher: CSHL Press
ISBN:
Category : Medical
Languages : en
Pages : 824
Get Book Here
Book Description
This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.
Author: Richard J. Simpson
Publisher: CSHL Press
ISBN:
Category : Medical
Languages : en
Pages : 824
Get Book Here
Book Description
This manual complements Simpson's Proteins and Proteomics manual, with a comprehensive collection of methods for protein purification from a variety of source preparations. The chapters include detailed protocols, methods for optimizing the performance of experiments, discussion of potential pitfalls, and troubleshooting advice.
Author: Richard J. Simpson
Publisher: CSHL Press
ISBN: 9780879695545
Category : Proteins
Languages : en
Pages : 946
Get Book Here
Book Description
Introduction to proteomics; one-dimensional polyacrylamide gel electrophoresis; preparing cellular and subcellular extracts; preparative two-dimensional gel electrophoresis with immobilized pH gradients; reversed-phase high -performance liquid chromatography; amino- and carboxy-terminal sequence analysis; peptide mapping and sequence analysis of gel-resolved proteins; the use of mass spectrometry in proteomics; proteomic methods for phosphorylation site mapping; characterization of protein complexes; making sense of proteomics - using bioinformatics to discover a protein's structure, functions, and interactions.
Author: Richard R Burgess
Publisher: Academic Press
ISBN: 0080923178
Category : Science
Languages : en
Pages : 915
Get Book Here
Book Description
Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Author:
Publisher:
ISBN: 9787030133878
Category : Proteins
Languages : en
Pages : 801
Get Book Here
Book Description
Author: Paul Cutler
Publisher: Springer Science & Business Media
ISBN: 159259655X
Category : Science
Languages : en
Pages : 474
Get Book Here
Book Description
The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Author: Richard J. Simpson
Publisher:
ISBN: 9780879698683
Category : Proteins
Languages : en
Pages : 0
Get Book Here
Book Description
The methods found here are drawn from such popular laboratory manuals as "Proteins and Proteomics" and "Purifying Proteins for Proteomics." This volume contains an essential collection of purification methods using gel electrophoresis and column chromatography.
Author: Rajni Hatti-Kaul
Publisher: CRC Press
ISBN: 9780203911686
Category : Medical
Languages : en
Pages : 688
Get Book Here
Book Description
This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Author: Jan-Christer Janson
Publisher: John Wiley & Sons
ISBN: 9780471186267
Category : Medical
Languages : en
Pages : 712
Get Book Here
Book Description
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Author: Daniel R. Marshak
Publisher: CSHL Press
ISBN: 9780879693855
Category : Science
Languages : en
Pages : 420
Get Book Here
Book Description
Cold Spring Harbor Laboratory. Softcover manual of fundamental procedures commonly used in protein biochemistry, for reseachers. Plastic comb spiral binding.
Author: Oscar Alzate
Publisher: CRC Press
ISBN: 1420076264
Category : Medical
Languages : en
Pages : 356
Get Book Here
Book Description
In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we beg
