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Author: Murat Sunbul
Publisher: Humana
ISBN: 9781493995394
Category : Science
Languages : en
Pages : 231
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Book Description
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Author: Murat Sunbul
Publisher: Humana
ISBN: 9781493995394
Category : Science
Languages : en
Pages : 231
Get Book Here
Book Description
This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.
Author: Gregory R. Hancock
Publisher: Routledge
ISBN: 1317627792
Category : Education
Languages : en
Pages : 515
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Book Description
The Reviewer’s Guide to Quantitative Methods in the Social Sciences provides evaluators of research manuscripts and proposals in the social and behavioral sciences with the resources they need to read, understand, and assess quantitative work. 35 uniquely structured chapters cover both traditional and emerging methods of quantitative data analysis, which neither junior nor veteran reviewers can be expected to know in detail. The second edition of this valuable resource updates readers on each technique’s key principles, appropriate usage, underlying assumptions and limitations, providing reviewers with the information they need to offer constructive commentary on works they evaluate. Written by methodological and applied scholars, this volume is also an indispensable author’s reference for preparing sound research manuscripts and proposals.
Author: Jian Pei
Publisher: Springer
ISBN: 3319914588
Category : Computers
Languages : en
Pages : 845
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Book Description
This two-volume set LNCS 10827 and LNCS 10828 constitutes the refereed proceedings of the 23rd International Conference on Database Systems for Advanced Applications, DASFAA 2018, held in Gold Coast, QLD, Australia, in May 2018. The 83 full papers, 21 short papers, 6 industry papers, and 8 demo papers were carefully selected from a total of 360 submissions. The papers are organized around the following topics: network embedding; recommendation; graph and network processing; social network analytics; sequence and temporal data processing; trajectory and streaming data; RDF and knowledge graphs; text and data mining; medical data mining; security and privacy; search and information retrieval; query processing and optimizations; data quality and crowdsourcing; learning models; multimedia data processing; and distributed computing.
Author: Gary R. Rassel
Publisher: Taylor & Francis
ISBN: 1000816443
Category : Social Science
Languages : en
Pages : 401
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Book Description
Practical Research Methods for Nonprofit and Public Administrators, Second Edition, covers basic principles of research design, data collection, data analysis, reporting and ethical principles of research. The discussion describes the skills as they would be used in practice in a not-for-profit or public administration environment. The book gives pragmatic instructions for designing research to ensure that results will be accurate and administrators can have confidence in them. It covers techniques to assure that researchers are measuring what they intend to measure and in a way that will be useful and manageable. Sampling methods to find out about larger populations are discussed as are survey, interview, and focus group data collection procedures. The book describes how Geographic Information Systems (GIS) are used to obtain information on the locations of service needs and providers and to draw maps showing these. Participatory research methods including community needs assessments and asset mapping are discussed. The book includes chapters on statistical analysis, visual displays of data, reporting of results and evaluation of programs. The use of computer programs for analyzing data is detailed and illustrated by examples. The appropriate software for analysis and mapping is discussed. A chapter updates the ethics and government requirements for research. Each chapter includes examples and exercises to enhance learning. The book is intended for administrators in not-for-profit and public administration organizations, providing practical skills that can be used in many situations. It is also intended for use as a text in academic programs in administration and management.
Author: Adam Tornhill
Publisher: Pragmatic Bookshelf
ISBN: 1680505807
Category : Computers
Languages : en
Pages : 356
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Book Description
Are you working on a codebase where cost overruns, death marches, and heroic fights with legacy code monsters are the norm? Battle these adversaries with novel ways to identify and prioritize technical debt, based on behavioral data from how developers work with code. And that's just for starters. Because good code involves social design, as well as technical design, you can find surprising dependencies between people and code to resolve coordination bottlenecks among teams. Best of all, the techniques build on behavioral data that you already have: your version-control system. Join the fight for better code! Use statistics and data science to uncover both problematic code and the behavioral patterns of the developers who build your software. This combination gives you insights you can't get from the code alone. Use these insights to prioritize refactoring needs, measure their effect, find implicit dependencies between different modules, and automatically create knowledge maps of your system based on actual code contributions. In a radical, much-needed change from common practice, guide organizational decisions with objective data by measuring how well your development teams align with the software architecture. Discover a comprehensive set of practical analysis techniques based on version-control data, where each point is illustrated with a case study from a real-world codebase. Because the techniques are language neutral, you can apply them to your own code no matter what programming language you use. Guide organizational decisions with objective data by measuring how well your development teams align with the software architecture. Apply research findings from social psychology to software development, ensuring you get the tools you need to coach your organization towards better code. If you're an experienced programmer, software architect, or technical manager, you'll get a new perspective that will change how you work with code. What You Need: You don't have to install anything to follow along in the book. TThe case studies in the book use well-known open source projects hosted on GitHub. You'll use CodeScene, a free software analysis tool for open source projects, for the case studies. We also discuss alternative tooling options where they exist.
Author: Lindsay Groves
Publisher: Springer
ISBN: 3642412025
Category : Computers
Languages : en
Pages : 479
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Book Description
This book constitutes the refereed proceedings of the 15th International Conference on Formal Engineering Methods, ICFEM 2013, held in Queenstown, New Zealand, in October/November 2013. The 28 revised full papers together with 2 keynote speeches presented were carefully reviewed and selected from 88 submissions. The topics covered are abstraction and refinement, formal specification and modeling, program analysis, software verification, formal methods for software safety, security, reliability and dependability, tool development, integration and experiments involving verified systems, formal methods used in certifying products under international standards, and formal model-based development and code generation.
Author: TianQiao Liu
Publisher: Springer Nature
ISBN: 9819715148
Category :
Languages : en
Pages : 581
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Book Description
Author: Wang, John
Publisher: IGI Global
ISBN: 1605660116
Category : Computers
Languages : en
Pages : 2542
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Book Description
There are more than one billion documents on the Web, with the count continually rising at a pace of over one million new documents per day. As information increases, the motivation and interest in data warehousing and mining research and practice remains high in organizational interest. The Encyclopedia of Data Warehousing and Mining, Second Edition, offers thorough exposure to the issues of importance in the rapidly changing field of data warehousing and mining. This essential reference source informs decision makers, problem solvers, and data mining specialists in business, academia, government, and other settings with over 300 entries on theories, methodologies, functionalities, and applications.
Author: Rafael Saraiva Campos
Publisher: Artech House
ISBN: 1608078175
Category : Technology & Engineering
Languages : en
Pages : 369
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Book Description
This new resource presents a comprehensive view of radio-frequency (RF) positioning. The book is organized to allow readers to progress at a fast pace, from the fundamentals of RF positioning, to the use of advanced tools such as artificial intelligence algorithms and application development environments. The first part of the book covers the fundamentals of RF localization. The second part addresses the application of those fundamentals in several types of wireless networks and technologies as Cellular Networks, Wi-Fi, Bluetooth, Sensor Networks, Ultra Wide Band, and Global Navigation Satellite Systems. The third part brings several tools to allow rapid development of positioning applications for mobile devices, as well as to support implementation, usage, deployment, and research of localization algorithms. This book presents numerous MATLAB examples, accompanied by the corresponding MATLAB code, made available at the book website. The MATLAB code to most figures is also provided, as well as databases of measurements collected during experiments conducted both in cellular and Wi-Fi networks. The book also is accompanied by Android source codes of the example apps developed in Chapter 10.
Author: Hang Li
Publisher: Springer
ISBN: 3540686363
Category : Computers
Languages : en
Pages : 701
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Book Description
This book constitutes the thoroughly refereed post-conference proceedings of the 4th Asia Information Retrieval Symposium, AIRS 2008, held in Harbin, China, in May 2008. The 39 revised full papers and 43 revised poster papers presented were carefully reviewed and selected from 144 submissions. All current issues in information retrieval are addressed: applications, systems, technologies and theoretical aspects of information retrieval in text, audio, image, video and multi-media data. The papers are organized in topical sections on IR models image retrieval, text classification, chinese language processing, text processing, application of IR, machine learning, taxonomy, IR methods, information extraction, summarization, multimedia, Web IR, and text clustering.
