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Author: Luis M. de Pablos
Publisher: Humana
ISBN: 9781071616802
Category : Medical
Languages : en
Pages : 336
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Book Description
This detailed book provides a comprehensive series of innovative research techniques and methodologies applied to the parasite genomics research area, all applying different approaches to analyzing parasite genomes and furthering the study of genetic complexity and the mechanisms of regulation. Beginning with chapters on novel sequencing and the bioinformatics pipeline, the volume continues by exploring diagnostic approaches using genomic tools, host-parasite interactions, as well as the genomics of parasite-derived extracellular vesicles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Parasite Genomics: Methods and Protocols creates a detailed picture of genomic approaches for researchers seeking a better understanding of characterizing parasite nucleic acid content.
Author: John E. Hyde
Publisher: Taylor & Francis US
ISBN: 9780896032392
Category : Medical
Languages : en
Pages : 488
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Book Description
Experts present methods and protocols essential for understanding parasites at the molecular level. The protocols cover culturing techniques for the major experimental organisms, methods for isolating and processing nucleic acids and proteins, PCR-based protocols for parasite identification, gene isolation and mutation, antibody-based procedures, chromosome and epitope mapping, flow cytometry, RNA sequencing, and parasite transformation.
Author: Luis M. de Pablos
Publisher: Humana
ISBN: 9781071616802
Category : Medical
Languages : en
Pages : 336
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Book Description
This detailed book provides a comprehensive series of innovative research techniques and methodologies applied to the parasite genomics research area, all applying different approaches to analyzing parasite genomes and furthering the study of genetic complexity and the mechanisms of regulation. Beginning with chapters on novel sequencing and the bioinformatics pipeline, the volume continues by exploring diagnostic approaches using genomic tools, host-parasite interactions, as well as the genomics of parasite-derived extracellular vesicles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Parasite Genomics: Methods and Protocols creates a detailed picture of genomic approaches for researchers seeking a better understanding of characterizing parasite nucleic acid content.
Author: Sara E. Melville
Publisher: Humana
ISBN: 9781617373312
Category : Science
Languages : en
Pages : 0
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Book Description
Parasitic diseases remain a major health problem throughout the world, for both humans and animals. For many of us, our technologically advanced lifestyle has decreased the prevalence and transmission of parasitic diseases, but for the majority of the world’s population, they are ever present in homes, domestic animals, food, or the environment. The study of parasites and parasitic disease has a long and distinguished history. In some cases, it has been driven by the great importance of the presence of the parasite to the community, for example, those that affect our livestock. In other cases, it is clear that applied research has suffered for lack of funding because the parasite affects people with few resources, such as the rural poor in resource-poor countries. These instances include the so-called “neglected diseases,” as defined by the World Health Organization (WHO). Parasites have complicated life cycles, and a thorough understanding of the unique characteristics of a particular parasite species is vital in attempts to avoid, prevent, or cure infection or to alleviate symptoms. Of course, the biological characteristics that each parasite has developed to aid survival and transmission, to avoid destruction by the immune system, and to adapt to a changing environment are of lasting fascination to basic biologists as well. The elegance of these biological systems has ensured that the study of protozoan and metazoan parasites also remains an active field of research in countries where the diseases are not a threat to the population.
Author: Dongyou Liu
Publisher: CRC Press
ISBN: 1040067069
Category : Medical
Languages : en
Pages : 899
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Book Description
Traditionally, laboratory identification of parasites has relied upon various phenotypic procedures that detect their morphological, biological, and immunological features. Because these procedures tend to be time-consuming and technically demanding, molecular methods based on nucleic acid amplification technologies have been increasingly utilized
Author: Nader Rifai
Publisher: Elsevier
ISBN: 0128160624
Category : Science
Languages : en
Pages : 424
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Book Description
Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments. - Explains the principles and tools of molecular biology - Describes standard and state-of-the-art molecular techniques for obtaining qualitative and quantitative results - Provides a detailed description of current molecular applications used to solve diagnostics tasks
Author: Aaron G. Maule
Publisher: CABI
ISBN: 1845930398
Category : Science
Languages : en
Pages : 470
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Book Description
Parasitic flatworms include Cestodes (tapeworms) and trematodes (flukes, schistosomes, etc) and are the cause of a number of major diseases of medical and veterinary significance. Much recent research has focused on molecular biology and genomics. this book aims to review advances in our understanding of these and related topics such as flatworm biochemistry, immunology and physiology. Where appropriate, comparisons are made between different parasitic flatworms and between parasitic and free-living species. Contributors to the book include leading authorities from Europe, North and South America, and Australia.
Author: Jan R. Mead
Publisher: Humana
ISBN: 9781493997473
Category : Medical
Languages : en
Pages : 407
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Book Description
This book encompasses broad aspects of Cryptosporidium research with established methods that have been improved and expanded over the years as well as recent cutting-edge techniques. Within this collection are numerous molecular methods as well as protocols for genotyping and diagnostics, while also including room for in vitro cell culture techniques to address the issues with growing this difficult organism continuously. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Cryptosporidium: Methods and Protocols serves as an idea guide to the inherent challenges of working with cryptosporidial parasites to provide a foundation for new investigators to build upon. The chapter “Accessing Cryptosporidium Omic and Isolate Data via CryptoDB.org” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author: David T. John, MSPH, PhD
Publisher: Elsevier Health Sciences
ISBN: 0721647936
Category : Medical
Languages : en
Pages : 487
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Book Description
With a new two-color design, Markell and Voge's Medical Parasitology has an updated and fresh look that highlights the comprehensive material students have trusted for over 40 years. Completely redrawn line drawings and improved halftones provide visual examples related directly to the textual material. The content explores the etiologic agents of human disease belonging to the animal kingdom: protozoa, helminths (worms), and arthropods (insects and spiders), all of which are a significant cause of, or link to illness encountered both in tropical and temperate environments. In addition to providing detailed descriptions of these agents, this text deals with the clinical diseases they cause, their modes of acquisition, transmission and epidemiology, and their pathogenesis, diagnosis, treatment and prevention. Ten-page insert with full color plates of various parasites, eggs, and life cycles provides students with real-life examples of how parasites and their associated material appear in order to facilitate their identification in the laboratory. Summary Tables appear at the ends of the parasite/disease chapters to summarize the main features of the chapter and to present the salient information from the chapter to allow students better comprehension of the material. Life cycle drawings show progression of parasites from infancy to adult so students can recognize parasites at each stage of life. Disease distribution maps depict the global distribution of key parasites to help students see the global impact that various parasites have. The text explores arthropods both as parasites in their own right and as vectors or intermediate hosts for other parasites so students can understand the direct and indirect impact that they have on health. New two-color design gives the material a fresh look and highlights important details in illustrations. Improved illustrations include all line drawings redrawn with a second color added, as well as improved quality in the halftones. Thorough revision reflecting all the most recent research findings and the most cutting-edge techniques for diagnosis and treatment. Significant change in authorship with David John taking the role of lead editor, and with a new co-editor, William Petri, a proven expert, writer, and speaker in the field of parasitology.
Author: Anne M. Zajac
Publisher: John Wiley & Sons
ISBN: 0813820537
Category : Medical
Languages : en
Pages : 370
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Book Description
Veterinary Clinical Parasitology, Eighth Edition, prepared under the auspices of the American Association of Veterinary Parasitologists (AAVP), emphasizes the morphologic identification of both internal and external parasites of domestic animals. Focusing on the tests and information most relevant to daily practice, the book describes accurate, cost-effective techniques for diagnosing parasitic infections in animals. Including clear, easy-to-find information on the distribution, life cycle, and importance of each parasite, Veterinary Clinical Parasitology offers more than 450 images to aid with diagnosis. The Eighth Edition includes a new chapter on immunologic and molecular diagnosis, increased coverage of ticks and new sections on identification of microfilariae and larvae in diagnostic samples. The new edition also features expanded information on quantitative egg counts, detection of anthelmintic resistance and identification of ruminant strongylid larvae. Additional improvements include many new images throughout the book, revised taxonomic information, a new layout featuring tabs by section to improve user-friendliness, and a companion website offering the images from the book in PowerPoint at www.wiley.com/go/zajac. Veterinary Clinical Parasitology is a highly practical benchside reference invaluable to clinicians, technicians, and students.
Author: F. E. G. Cox
Publisher: John Wiley & Sons
ISBN: 1444313959
Category : Science
Languages : en
Pages : 294
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Book Description
This is a thorough revision and update of the highly successful first edition, which which achieved sales in excess of 4,500. The text serves as a comprehensive introduction to parasitology for both undergraduate and beginning graduate students. In this edition, particular emphasis is placed on parasites of human and veterinary importance. The first three chapters in the text are concerned with how parasites 'work,' their biochemistry, molecular and cell biology and physiology. The remaining chapters cover ecology and epidemiology, immunology and chemotherapy, with the final chapter covering integrated control. This new edition contains new material on cell and molecular biology, vectors and control, which is in contrast to the general biological approach of the first edition. The second edition will succeed the first as the major text on parasitology for students in biology, zoology, microbiology, medicine, veterinary medicine, tropical medicine and public health.
