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Author: Tuck Seng Wong
Publisher: Springer Nature
ISBN: 3030568989
Category : Science
Languages : en
Pages : 213
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Book Description
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Author: Tuck Seng Wong
Publisher: Springer Nature
ISBN: 3030568989
Category : Science
Languages : en
Pages : 213
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Book Description
This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.
Author: Huimin Zhao
Publisher: John Wiley & Sons
ISBN: 3527344705
Category : Science
Languages : en
Pages : 41
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Book Description
A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.
Author: Kristian Müller
Publisher: Springer Science & Business Media
ISBN: 1597451878
Category : Science
Languages : en
Pages : 318
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Book Description
Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.
Author: Henry C. Vogel
Publisher: William Andrew
ISBN: 0815517130
Category : Technology & Engineering
Languages : en
Pages : 831
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Book Description
This is a well-rounded handbook of fermentation and biochemical engineering presenting techniques for the commercial production of chemicals and pharmaceuticals via fermentation. Emphasis is given to unit operations fermentation, separation, purification, and recovery. Principles, process design, and equipment are detailed. Environment aspects are covered. The practical aspects of development, design, and operation are stressed. Theory is included to provide the necessary insight for a particular operation. Problems addressed are the collection of pilot data, choice of scale-up parameters, selection of the right piece of equipment, pinpointing of likely trouble spots, and methods of troubleshooting. The text, written from a practical and operating viewpoint, will assist development, design, engineering and production personnel in the fermentation industry. Contributors were selected based on their industrial background and orientation. The book is illustrated with numerous figures, photographs and schematic diagrams.
Author: Glyn O. Phillips
Publisher: Elsevier
ISBN: 0857093630
Category : Technology & Engineering
Languages : en
Pages : 457
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Book Description
Traditionally a source of nutrition, proteins are also added to foods for their ability to form gels and stabilise emulsions, among other properties. The range of specialised protein ingredients used in foods is increasing. Handbook of food proteins provides an authoritative overview of the characteristics, functionalities and applications of different proteins of importance to the food industry in one convenient volume.The introductory chapter provides an overview of proteins and their uses in foods. The following chapters each focus on a particular protein ingredient or group of ingredients covering their origins, production, properties and applications. The proteins discussed are caseins, whey proteins, gelatin and other meat-derived protein ingredients, seafood proteins, egg proteins, soy proteins, pea and other legume proteins, mycoprotein, wheat gluten, canola and other oilseed proteins, algal proteins and potato protein. A chapter on texturised vegetable proteins completes the volume. Innovative products and potential methods for improving nutrition and diet using these proteins are described.With its distinguished editors and international team of expert contributors Handbook of food proteins is an invaluable reference tool for professionals using food protein ingredients for both food and other applications. - An authoritative overview of the characteristics, functionalities and applications of different proteins of importance to the food industry - Chapters each focus on a particular protein ingredient or group of ingredients - Innovative products and potential methods for improving nutrition and diet using proteins is also described
Author: John M. Walker
Publisher: Springer Science & Business Media
ISBN: 0896039404
Category : Medical
Languages : en
Pages : 1446
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Book Description
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Author: Xincheng Zhang
Publisher: John Wiley & Sons
ISBN: 1119158974
Category : Technology & Engineering
Languages : en
Pages : 859
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Book Description
A comprehensive resource containing the operating principles and key insights of LTE networks performance optimization LTE Optimization Engineering Handbook is a comprehensive reference that describes the most current technologies and optimization principles for LTE networks. The text offers an introduction to the basics of LTE architecture, services and technologies and includes details on the key principles and methods of LTE optimization and its parameters. In addition, the author clarifies different optimization aspects such as wireless channel optimization, data optimization, CSFB, VoLTE, and video optimization. With the ubiquitous usage and increased development of mobile networks and smart devices, LTE is the 4G network that will be the only mainstream technology in the current mobile communication system and in the near future. Designed for use by researchers, engineers and operators working in the field of mobile communications and written by a noted engineer and experienced researcher, the LTE Optimization Engineering Handbook provides an essential guide that: Discusses the latest optimization engineering technologies of LTE networks and explores their implementation Features the latest and most industrially relevant applications, such as VoLTE and HetNets Includes a wealth of detailed scenarios and optimization real-world case studies Professionals in the field will find the LTE Optimization Engineering Handbook to be their go-to reference that includes a thorough and complete examination of LTE networks, their operating principles, and the most current information to performance optimization.
Author:
Publisher: Elsevier
ISBN: 0080930743
Category : Mathematics
Languages : en
Pages : 1473
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Book Description
The Handbook Philosophy of Technology and Engineering Sciences addresses numerous issues in the emerging field of the philosophy of those sciences that are involved in the technological process of designing, developing and making of new technical artifacts and systems. These issues include the nature of design, of technological knowledge, and of technical artifacts, as well as the toolbox of engineers. Most of these have thus far not been analyzed in general philosophy of science, which has traditionally but inadequately regarded technology as mere applied science and focused on physics, biology, mathematics and the social sciences. - First comprehensive philosophical handbook on technology and the engineering sciences - Unparalleled in scope including explorative articles - In depth discussion of technical artifacts and their ontology - Provides extensive analysis of the nature of engineering design - Focuses in detail on the role of models in technology
Author: Joseph D. Bronzino
Publisher: CRC Press
ISBN: 1439863113
Category : Medical
Languages : en
Pages : 5245
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Book Description
The definitive bible for the field of biomedical engineering, this collection of volumes is a major reference for all practicing biomedical engineers and students. Now in its fourth edition, this work presents a substantial revision, with all sections updated to offer the latest research findings. New sections address drugs and devices, personalized medicine, and stem cell engineering. Also included is a historical overview as well as a special section on medical ethics. This set provides complete coverage of biomedical engineering fundamentals, medical devices and systems, computer applications in medicine, and molecular engineering.
Author: Uwe T. Bornscheuer
Publisher: Humana Press
ISBN: 9781493973644
Category : Science
Languages : en
Pages : 350
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Book Description
This volume details basic and advanced protocols for both stages of protein engineering: the library design phase and the identification of improved variants by screening and selection. Chapters focus on enzyme engineering using rational and semi-rational approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Engineering: Methods and Protocols aims to aid scientists in the planning and performance of their experiments. The chapter 'Functional Analysis of Membrane Proteins Produced by Cell-Free Translation' is open access under a CC BY 4.0 license via link.springer.com.
