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Author: Carmichael J.A. Wallace
Publisher: CRC Press
ISBN: 9780849347276
Category : Medical
Languages : en
Pages : 300
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Book Description
As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features
Author: Carmichael J.A. Wallace
Publisher: CRC Press
ISBN: 9780849347276
Category : Medical
Languages : en
Pages : 300
Get Book Here
Book Description
As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features
Author: Zuzana Machova
Publisher:
ISBN:
Category :
Languages : en
Pages : 158
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Book Description
Author: Carissa M Soto
Publisher: Frontiers Media SA
ISBN: 2889193950
Category : Chemistry
Languages : en
Pages : 121
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Book Description
In the past 20 years protein engineering has been used for the production of proteins mostly for biological applications. The incorporation of artificial amino acids and chemical handles into proteins had made possible the design and production of protein-based materials like hybrid inorganic-organic materials, smart/ responsive materials, monodisperse polymers, and nanoscale assemblies. In the current topic, we cover current uses and envision future applications of materials generated using protein engineering and biosynthesis techniques. I would like to acknowledge the U.S. Office of Naval Research for financial support and Dr. Cherise Bernard for her contributions during the early stages of the Research Topic.
Author: Saran A. Narang
Publisher: Butterworth-Heinemann
ISBN: 1483161285
Category : Technology & Engineering
Languages : en
Pages : 285
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Book Description
Protein Engineering: Approaches to the Manipulation of Protein Folding outlines the complexity of the protein-folding problem and the potential of using genetic tools which, in combination with physical techniques, are expected to shed new light. The book begins with an overview of the basic concepts of protein folding, along with prediction methods and protein-folding models. Separate chapters cover experimental approaches to in vitro protein folding; general approaches used to characterize the folding reaction, equilibrium and kinetic experiments; and strategies employed to elucidate structure/function relationships in proteins of unknown tertiary structure. Subsequent chapters cover the structural and functional features of the HIV envelope protein; x-ray diffraction of proteins; application of Fourier transform infrared (FT-IR) spectroscopy to probe the secondary structure and orientation of membrane-associated proteins; and fluorescence measurements of proteins. The final chapters discuss nuclear magnetic resonance studies of proteins and the potential of the synthetic gene approach applied to the problem of protein folding.
Author: Michael R. Haugbro
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Deciphering the roles that protein post-translational modifications, protein-protein interactions, and protein dynamics play in modulating protein structure and function requires the ability to introduce amino acid modifications, cross-linking moieties, biophysical probes, and other synthetic chemical species into proteins. Current technologies for the semi-synthesis of modified proteins are optimized for in vitro reactions and are not easily transferable to more biologically relevant systems such as live cell culture. Analogous methodologies that work in situ rely on genetic manipulations that often result in low resolution data and a high level of off-target effects. Early attempts to address this disconnect have employed protein ligase systems such as Sortase and split inteins, but as of yet have not become robust enough for wide-spread use. In this thesis we expand upon the existing technologies and introduce new methods for the precise chemical manipulation of proteins in cell culture using split inteins. We applied in-nucleo protein trans-splicing to determine the in situ interactome of histone PTMs, combining chemical precision and native protein environment in an unprecedented manner unattainable by any other approach. The full results of the proteomics experiments enabled by this technique can be found in Appendix 2, available with the digital version of this thesis. We also expanded the in situ PTS toolbox by developing a multiplexed method for histone labeling in nuclei and in live cells. We demonstrated this capability by labeling two histones with different color fluorophores in a completely orthogonal manner. Finally, we utilized an atypical split intein known as VidaL to achieve the first semi-synthesis of a modified protein in cells, generating histones bearing multiple PTMs and an affinity handle in the chromatin of live cells. We added to this platform by combining our semi-synthesis capabilities with control of the subcellular localization of our target protein, in a single reaction. We envision that these new methods described in this thesis will equip protein chemists and cellular biologists alike with new capabilities for the study of protein structure and function.
Author: Robin E. Offord
Publisher: John Wiley & Sons
ISBN:
Category : Science
Languages : en
Pages : 256
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Book Description
Author: Miquel Vila-Perelló
Publisher: Humana
ISBN: 9781071604335
Category : Science
Languages : en
Pages : 0
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Book Description
This book provides a comprehensive overview of Expressed Protein Ligation (EPL), detailing methods and protocols to generate site-specifically modified proteins. Chapters include an overview of the protein semi-synthesis field, as well as related areas that have contributed to the development of EPL such as protein splicing and peptide synthesis. Following the introductory chapters, the rest of the book guides readers through protocols to perform EPL reactions, methods to synthesize peptide thioesters and to perform peptide and protein ligations, label proteins inside living cells, protocols for the semi-synthesis of phorphorylated, glycosylated and ubiquitylated proteins, synthesis and assembly of assymetrically modified nucleosomes, use of ligation auxiliaries and synthesis of cyclic proteins, as well as novel desulfurization strategies and use of selective Cys side chain protection to obtain precisely modified proteins.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Expressed Protein Ligation: Methods and Protocols will ensure successful implementation of protein semi-synthesis methods to further study the structure and function of proteins.
Author: Huimin Zhao
Publisher: John Wiley & Sons
ISBN: 3527344705
Category : Science
Languages : en
Pages : 41
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Book Description
A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.
Author: Lilia Alberghina
Publisher: CRC Press
ISBN: 1482283484
Category : Medical
Languages : en
Pages : 393
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Book Description
Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a sec
Author: Ashraf Brik
Publisher: John Wiley & Sons
ISBN: 3527346600
Category : Science
Languages : en
Pages : 626
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Book Description
How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.
