Stable Isotope Probing

Stable Isotope Probing PDF Author: Marc G. Dumont
Publisher: Humana
ISBN: 9781493997237
Category : Science
Languages : en
Pages : 247

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Book Description
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Stable Isotope Probing

Stable Isotope Probing PDF Author: Marc G. Dumont
Publisher: Humana
ISBN: 9781493997237
Category : Science
Languages : en
Pages : 247

Get Book Here

Book Description
This book provides definitive methods to perform stable isotope probing (SIP) experiments, covering a wide spectrum of stable isotope techniques used in microbial ecology, such as methods to target and analyze labeled DNA, rRNA, mRNA, protein, and PLFA. Protocols to study stable isotope fractionation by microbial pathways, the analysis of labeled communities with Raman microscopy, Chip-SIM, as well as quantitative SIP (qSIP) and high-resolution SIP (HR-SIP) are also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Stable Isotope Probing: Methods and Protocols provides readers with up-to-date protocols ranging from basic to the most sophisticated applications of SIP and will benefit anyone pursuing this exciting area of study.

Probing the Limits of Representation

Probing the Limits of Representation PDF Author: Saul Friedländer
Publisher: Harvard University Press
ISBN: 9780674707665
Category : History
Languages : en
Pages : 420

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Book Description
German memory, judicial interrogation, and historical reconstruction : writing perpetrator history from postwar testimony / Christopher R. Browning -- Historical emplotment and the problem of truth / Hayden White -- On emplotment : two kinds of ruin / Perry Anderson -- History, counterhistory, and narrative / Amos Funkenstein -- Just one witness / Carlo Ginzburg -- Of plots, witnesses, and judgments / Martin Jay -- Representing the Holocaust : reflections on the historians' debate / Dominick LaCapra -- Historical understanding and counterrationality : the Judenrat as epistemological vantage / Dan Diner -- History beyond the pleasure principle : some thoughts on the representation of trauma / Eric L. Santner -- Habermas, enlightenment, and antisemitism / Vincent P. Pecora -- Between image and phrase : progressive history and the "final solution" as dispossession / Sande Cohen.; Science, modernity, and the "final solution" / Mario Biagioli -- Holocaust and the end of history : postmodern historiography in cinema / Anton Kaes -- Whose story is it, anyway? : ideology and psychology in the representation of the Shoah in Israeli literature / Yael S. Feldman -- Translating Paul Celan's "Todesfuge" : rhythm and repetition as metaphor / John Felstiner -- "The grave in the air" : unbound metaphors in post-Holocaust poetry / Sidra DeKoven Ezrahi -- The dialectics of unspeakability : language, silence, and the narratives of desubjectification / Peter Haidu -- The representation of limits / Berel Lang -- The book of the destruction / Geoffrey H. Hartman.

Cognitive Interviewing

Cognitive Interviewing PDF Author: Gordon B. Willis
Publisher: SAGE Publications
ISBN: 1483389308
Category : Social Science
Languages : en
Pages : 323

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Book Description
"As both an academic instructor in questionnaire design and a research design methodologist for the federal government, I feel this book is very timely, useful for students and practitioners, and unique in its use of real world practical examples that most everyone can relate." —Terry Richardson, General Accounting Office "The combination of theory and practical application will make this a useful book for students as well as professionals who want to learn how to incorporate cognitive interviewing into the questionnaire design process." —Rachel Caspar, RTI International The design and evaluation of questionnaires—and of other written and oral materials—is a challenging endeavor, fraught with potential pitfalls. Cognitive Interviewing: A Tool for Improving Questionnaire Design describes a means of systematically developing survey questions through investigations that intensively probe the thought processes of individuals who are presented with those inquiries. The work provides general guidance about questionnaire design, development, and pre-testing sequence, with an emphasis on the cognitive interview. In particular, the book gives detailed instructions about the use of verbal probing techniques, and how one can elicit additional information from subjects about their thinking and about the manner in which they react to tested questions. These tools help researchers discover how well their questions are working, where they are failing, and determine what they can do to rectify the wide variety of problems that may surface while working with questionnaires. Cognitive Interviewing is ideally suited as a course text for advanced undergraduate and graduate research courses across the social sciences. Professional researchers and faculty in the social sciences, as well as practice fields such as medicine, business, and education, will also find this an invaluable reference for survey research. There is no other book on the market that covers cognitive interviewing as applied to questionnaire design.

Probing Experience

Probing Experience PDF Author: Joyce Westerink
Publisher: Springer
ISBN: 9789048176755
Category : Computers
Languages : en
Pages : 0

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Book Description
This book adheres to the vision that in the future compelling user experiences will be key differentiating benefits of products and services. It is the first book to combine academic and business viewpoints on measuring user experiences for product development. The book gathers authors from different backgrounds. This is a mosaic of their work, and that of Philips Research, in the assessment of user experience, covering the full range from academic research to commercial propositions.

Probing the Sky

Probing the Sky PDF Author: Curtis Peebles
Publisher:
ISBN: 9781626830219
Category : Research aircraft
Languages : en
Pages : 334

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Book Description
With the development of supersonic aircraft, the X-plane era ushered in a new and challenging phase of flight. Researchers found that much of the knowledge accumulated from the previous, subsonic flight era did not apply to the emerging supersonic aircraft. These turbojet-powered planes also outpaced the usefulness of the wind tunnel, previously an indispensible tool of aeronautic research.This book explores the development of the X-series research aircraft, the planes that helped bridge the gap between subsonic flight and hypersonic flight.

Probing the Sky with Radio Waves

Probing the Sky with Radio Waves PDF Author: Chen-Pang Yeang
Publisher: University of Chicago Press
ISBN: 022601519X
Category : Science
Languages : en
Pages : 378

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Book Description
By the late nineteenth century, engineers and experimental scientists generally knew how radio waves behaved, and by 1901 scientists were able to manipulate them to transmit messages across long distances. What no one could understand, however, was why radio waves followed the curvature of the Earth. Theorists puzzled over this for nearly twenty years before physicists confirmed the zig-zag theory, a solution that led to the discovery of a layer in the Earth’s upper atmosphere that bounces radio waves earthward—the ionosphere. In Probing the Sky with Radio Waves, Chen-Pang Yeang documents this monumental discovery and the advances in radio ionospheric propagation research that occurred in its aftermath. Yeang illustrates how the discovery of the ionosphere transformed atmospheric science from what had been primarily an observational endeavor into an experimental science. It also gave researchers a host of new theories, experiments, and instruments with which to better understand the atmosphere’s constitution, the origin of atmospheric electricity, and how the sun and geomagnetism shape the Earth’s atmosphere. This book will be warmly welcomed by scholars of astronomy, atmospheric science, geoscience, military and institutional history, and the history and philosophy of science and technology, as well as by radio amateurs and electrical engineers interested in historical perspectives on their craft.

Probing (Harbingers)

Probing (Harbingers) PDF Author: Frank Peretti
Publisher: Bethany House
ISBN: 1441231471
Category : Fiction
Languages : en
Pages : 376

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Book Description
Myers, Peretti, Hunt, and Gansky Offer Latest Harbingers Volume Cycle Three of the Harbingers series offers more suspense, more chills, and a deeper look into the battle for light in a growing darkness. In Myers's "Leviathan," the team heads to Hollywood for a taping of the new TV pilot, Live or Die, the Ultimate Reality. Little do they realize the depths of darkness they are about to enter--a darkness that, unless they stop it, will soon spread across the globe. Frank Peretti's "The Mind Pirates" offers a rousing story featuring bizarre visions and memories of a murder, a kidnapping by 17th-century pirates, and an earring with mysterious powers. The team must overcome the ruthless scheming of an evil, hidden nemesis. In "Hybrids" from Angela Hunt, the sight of two children chills the team to their bones. Seeking rest and relaxation, the four friends must instead find answers to the arrival and mission of two mysterious black-eyed children. In "The Village" from Alton Gansky, a visit to a guarded and secretive small town in North Carolina becomes the most challenging mystery they've ever faced--as they race to solve a problem they barely understand before time runs out.

Probing the Skin

Probing the Skin PDF Author: Dirk Vanderbeke
Publisher: Cambridge Scholars Publishing
ISBN: 144387518X
Category : Social Science
Languages : en
Pages : 352

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Book Description
Adopting an interdisciplinary approach, this volume explores representations of skin in literature, art, art history, visual media, and medicine and its history. The essays collected here probe the symbolic potential of skin as a shifting sign in various historical and cultural contexts, and also examine the material and organic properties of the body’s largest organ. They deal with skin as a sensual organ, as an interface or contact zone, as the visual marker of identity, and as a lieu de memoire in different periods and media. In its material characteristics, skin is regarded as a medium, a canvas, a surface, and an object of both artistic and medical investigations. The contributions investigate representations of skin in sculpture, painting, film, and fictional, as well as non-fictional, texts from the 16th century to the present. The topics addressed here include the problematic representation of racial identity via skin colour in various media; the sensual qualities of the skin, such as smell or taste; the form and function of tattoos as markers of personal, as well as collective, identity; and scars as signifiers of personal pain and collective suffering.

Stable Isotope Probing and Related Technologies

Stable Isotope Probing and Related Technologies PDF Author: J. Colin Murrell
Publisher: American Society for Microbiology Press
ISBN: 1555815375
Category : Science
Languages : en
Pages : 508

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Book Description
The ideal starting point for investigating, developing, and implementing stable isotope technologies. • Guides researchers through basic, tested, and proven protocols including DNA, RNA, protein, and phospholipid fatty acid (PLFA) SIP, from concept and history through detailed methodology, troubleshooting, and interpretation to optimal and future uses. • Explores important and emerging applications of SIP in environmental microbiology, ranging from bioremediation and gene mining to carbon tracking and gut microflora function. • Examines explorations of further elegant isotope labeling technologies such as Raman-FISH, NanoSIMS, and isotope arrays. • Serves as a valuable resource for environmental microbiology students and researchers and genomics, biotechnology, and medical microbiology professionals.

Probing the Ethics of Holocaust Culture

Probing the Ethics of Holocaust Culture PDF Author: Claudio Fogu
Publisher: Harvard University Press
ISBN: 0674973267
Category : History
Languages : en
Pages : 528

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Book Description
Depictions of the Holocaust in history, literature, and film became a focus of intense academic debate in the 1980s and 1990s. Today, with the passing of the eyewitness generation and the rise of comparative genocide studies, the Holocaust’s privileged place not only in scholarly discourse but across Western society has been called into question. Probing the Ethics of Holocaust Culture is a searching reappraisal of the debates and controversies that have shaped Holocaust studies over a quarter century. This landmark volume brings international scholars of the founding generation of Holocaust studies into conversation with a new generation of historians, artists, and writers who have challenged the limits of representation through their scholarly and cultural practices. Focusing on the public memorial cultures, testimonial narratives, and artifacts of cultural memory and history generated by Holocaust remembrance, the volume examines how Holocaust culture has become institutionalized, globalized, and variously contested. Organized around three interlocking themes—the stakes of narrative, the remediation of the archive, and the politics of exceptionality—the essays in this volume explore the complex ethics surrounding the discourses, artifacts, and institutions of Holocaust remembrance. From contrasting viewpoints and, in particular, from the multiple perspectives of genocide studies, the authors question if and why the Holocaust should remain the ultimate test case for ethics and a unique reference point for how we understand genocide and crimes against humanity.