Marine Microbiomes: Towards Standard Methods and Best Practices

Marine Microbiomes: Towards Standard Methods and Best Practices PDF Author: Stéphane Pesant
Publisher: Frontiers Media SA
ISBN: 2832541313
Category : Science
Languages : en
Pages : 125

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Book Description
A decade of technological advances and research on the human microbiome has re-defined our understanding of biological systems, and now offers diagnostic tools and new approaches to human health. Likewise, marine ecosystems are driven by their microbiome, the ensemble of microscopic organisms that inhabit the water column, sediments and aquatic organisms, and regulate most fluxes of energy and matter. While the human microbiome is composed principally of bacteria, the marine microbiome has a much broader ensemble of microscopic organisms with sizes spanning from viruses of a few tens of nanometres to metazoans of several centimetres. Advances in high throughput imaging and sequencing are emerging in aquatic science, providing important insights into ecosystem structure and functions, and contributing to develop new indicators of ecosystem health and potential hazards to living resources and humans. A number of research exploration and monitoring programs such as the Tara Oceans global expedition and the Marine Biological Observing Network (MBON), and the rising popularity of environmental DNA (eDNA) already led to a wealth of data. However, the wide range of methodologies used in this early exploration phase poses a challenge to integrative studies, prompting community responses such as the Genomic Standards Consortium. This Research Topic will foster cross-community exchange of standards and best practices. It is an opportunity for the different communities working on marine microbiomes to discuss the advantages and the limitations of their sampling and analysis methods. The collection of papers will constitute a knowledge base aimed at (1) addressing the integration of existing observations from heterogenous methods and data types, and (2) working towards cross-communities standards and best practices for future observations and experiments. This Research Topic will publish comprehensive methodological papers that review a sufficiently large body of field or experimental work. Supporting (meta)data, such as environmental context, sampling materials, sample treatments, extraction and imaging yields, must be provided and should preferably be uploaded to an online sample registry such as BioSamples. Protocols and analytical methods should preferably be uploaded to an online repository such as Protocols.io. We welcome contributions from communities working in different environments: · focus on marine systems, but also interested in estuarine and freshwater systems · coastal, open ocean and deep-sea regions · benthic, pelagic and aerosol environments We strongly encourage contributions from communities involved in: · networks of local observatories and long-term monitoring programs · regional & basin-scale surveys, and global expeditions · biodiscovery linked to economic sectors such as pharmaceutics and bioengineering · environmental and health assessments linked to economic sectors such as aquaculture, fisheries, seabed mining, wind-farms, and oil and gas

Marine Microbiomes: Towards Standard Methods and Best Practices

Marine Microbiomes: Towards Standard Methods and Best Practices PDF Author: Stéphane Pesant
Publisher: Frontiers Media SA
ISBN: 2832541313
Category : Science
Languages : en
Pages : 125

Get Book Here

Book Description
A decade of technological advances and research on the human microbiome has re-defined our understanding of biological systems, and now offers diagnostic tools and new approaches to human health. Likewise, marine ecosystems are driven by their microbiome, the ensemble of microscopic organisms that inhabit the water column, sediments and aquatic organisms, and regulate most fluxes of energy and matter. While the human microbiome is composed principally of bacteria, the marine microbiome has a much broader ensemble of microscopic organisms with sizes spanning from viruses of a few tens of nanometres to metazoans of several centimetres. Advances in high throughput imaging and sequencing are emerging in aquatic science, providing important insights into ecosystem structure and functions, and contributing to develop new indicators of ecosystem health and potential hazards to living resources and humans. A number of research exploration and monitoring programs such as the Tara Oceans global expedition and the Marine Biological Observing Network (MBON), and the rising popularity of environmental DNA (eDNA) already led to a wealth of data. However, the wide range of methodologies used in this early exploration phase poses a challenge to integrative studies, prompting community responses such as the Genomic Standards Consortium. This Research Topic will foster cross-community exchange of standards and best practices. It is an opportunity for the different communities working on marine microbiomes to discuss the advantages and the limitations of their sampling and analysis methods. The collection of papers will constitute a knowledge base aimed at (1) addressing the integration of existing observations from heterogenous methods and data types, and (2) working towards cross-communities standards and best practices for future observations and experiments. This Research Topic will publish comprehensive methodological papers that review a sufficiently large body of field or experimental work. Supporting (meta)data, such as environmental context, sampling materials, sample treatments, extraction and imaging yields, must be provided and should preferably be uploaded to an online sample registry such as BioSamples. Protocols and analytical methods should preferably be uploaded to an online repository such as Protocols.io. We welcome contributions from communities working in different environments: · focus on marine systems, but also interested in estuarine and freshwater systems · coastal, open ocean and deep-sea regions · benthic, pelagic and aerosol environments We strongly encourage contributions from communities involved in: · networks of local observatories and long-term monitoring programs · regional & basin-scale surveys, and global expeditions · biodiscovery linked to economic sectors such as pharmaceutics and bioengineering · environmental and health assessments linked to economic sectors such as aquaculture, fisheries, seabed mining, wind-farms, and oil and gas

Handbook of Molecular Microbial Ecology I

Handbook of Molecular Microbial Ecology I PDF Author: Frans J. de Bruijn
Publisher: John Wiley & Sons
ISBN: 1118010493
Category : Science
Languages : en
Pages : 1155

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Book Description
The premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the first comprehensive two-volume reference to cover unculturable microorganisms in a large variety of habitats, which could not previously have been analyzed without metagenomic methodology. It features review articles as well as a large number of case studies, based largely on original publications and written by international experts. This first volume, Metagenomics and Complementary Approaches, covers such topics as: Background information on DNA reassociation and use of 16 rRNA and other DNA fingerprinting approaches Species designation in microbiology Metagenomics: Introduction to the basic tools with examples Consortia and databases Bioinformatics Computer-assisted analysis Complementary approaches—microarrays, metatranscriptomics, metaproteomics, metabolomics, and single cell analysis A special feature of this volume is the highlighting of the databases and computer programs used in each study; they are listed along with their sites in order to facilitate the computer-assisted analysis of the vast amount of data generated by metagenomic studies. Handbook of Molecular Microbial Ecology I is an invaluable reference for researchers in metagenomics, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; molecular microbial ecologists; and professionals in molecular microbiology and bioinformatics.

Biology of Skates

Biology of Skates PDF Author: David A. Ebert
Publisher: Springer Science & Business Media
ISBN: 1402097034
Category : Science
Languages : en
Pages : 242

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Book Description
Skates have become a concern in recent years due to the preponderance of these elasmobranchs that are caught as bycatch or as a directed fishery. This has raised concern because skates have life history characteristics that may make them vulnerable to over-exploitation. It was due to this concern that prompted Drs. David Ebert and James Sulikowski to organize an international symposium on the “Biology of Skates”. The aims and goals of the symposium were to bring together an international group of researchers to meet, discuss, perhaps develop collaborations, and present their most recent findings. The symposium was held over two days, on 13-14 July, 2006, in conjunction with the 22nd annual meeting of the American Elasmobranch Society in New Orleans, LA. A total of 31 authors from four countries contributed 16 papers that appear in this volume. The papers are broadly arranged into four separate categories: systematics and biogeography, diet and feeding ecology, reproductive biology, and age and growth. This is the first dedicated book on the biology of skates. We hope that readers will find this volume of interest and that it helps encourage and stimulate future research into these fascinating fishes.

Methods in Environmental Forensics

Methods in Environmental Forensics PDF Author: Stephen M. Mudge
Publisher: CRC Press
ISBN: 1420006053
Category : Law
Languages : en
Pages : 406

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Book Description
While environmental catastrophes can be naturally occurring, often they are the result of criminal intent or malfeasance. Sorting out the details when the land itself is the only witness requires a special set of investigative skills. For accountability to be established, investigators must be able to answer these questions with a measure of scient

In Vitro Transcription and Translation Protocols

In Vitro Transcription and Translation Protocols PDF Author: Guido Grandi
Publisher: Springer Science & Business Media
ISBN: 1588295583
Category : Medical
Languages : en
Pages : 568

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Book Description
This book is a highly anticipated update of the previous edition. It provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. It has been completely updated with new chapters and topics.

Myc as a Disease Target Beyond Cancer

Myc as a Disease Target Beyond Cancer PDF Author: Jonathan R. Whitfield
Publisher: Frontiers Media SA
ISBN: 2832553958
Category : Science
Languages : en
Pages : 216

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Book Description
Myc is a pleiotropic transcription factor fundamental for many cellular processes. It is an infamous and highly-studied oncogene, with around 10,000 articles published in the last decade. The majority of these link it to cancer. The focus on Myc as a hot target in cancer has led to immense efforts – from academia through to pharmaceutical companies – being directed towards finding a Myc inhibitor. Not surprisingly, given its fundamental nature in many physiological processes, Myc has also been linked to a large variety of pathologies other than cancer. The growing efforts to develop Myc inhibitors as cancer therapies will have important implications for the treatment of these diseases too, whether they have a clearly-demonstrated aetiology linked with Myc, or so far are only at early stages of association with it. Interestingly, the activation of Myc may also prove helpful in the field of regenerative medicine and reversal of ageing. The aim of this Research Topic is therefore to describe non-oncological pathologies and conditions to which Myc modulation could be applied. These will include conditions that have strong links to Myc and where Myc modulation has already been modelled, as well as conditions that so far have more limited data linking them to Myc but where it seems to have a key role. Authors will be expected to briefly review the particular disease and what is known to link Myc to it, plus the possibility of any clinical trials in the near future

Toward a Sustainable Agriculture Through Plant Biostimulants

Toward a Sustainable Agriculture Through Plant Biostimulants PDF Author: Youssef Rouphael
Publisher: MDPI
ISBN: 3036500286
Category : Science
Languages : en
Pages : 708

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Book Description
Over the past decade, interest in plant biostimulants has been on the rise, compelled by the growing interest of researchers, extension specialists, private industries, and farmers in integrating these products in the array of environmentally friendly tools to secure improved crop performance, nutrient efficiency, product quality, and yield stability. Plant biostimulants include diverse organic and inorganic substances, natural compounds, and/or beneficial microorganisms such as humic acids, protein hydrolysates, seaweed and plant extracts, silicon, endophytic fungi like mycorrhizal fungi, and plant growth-promoting rhizobacteria belonging to the genera Azospirillum, Azotobacter, and Rhizobium. Other substances (e.g., chitosan and other biopolymers and inorganic compounds) can have biostimulant properties, but their classification within the group of biostimulants is still under consideration. Plant biostimulants are usually applied to high-value crops, mainly greenhouse crops, fruit trees and vines, open-field crops, flowers, and ornamentals to sustainably increase yield and product quality. The global biostimulant market is currently estimated at about $2.0 billion and is expected to reach $3.0 billion by 2021 at an annual growth rate of 13%. A growing interest in plant biostimulants from industries and scientists was demonstrated by the high number of published peer-reviewed articles, conferences, workshops, and symposia in the past ten years. This book compiles several original research articles, technology reports, methods, opinions, perspectives, and invited reviews and mini reviews dissecting the biostimulatory action of these natural compounds and substances and beneficial microorganisms on crops grown under optimal and suboptimal growing conditions (e.g., salinity, drought, nutrient deficiency and toxicity, heavy metal contaminations, waterlogging, and adverse soil pH conditions). Also included are contributions dealing with the effect as well as the molecular and physiological mechanisms of plant biostimulants on nutrient efficiency, product quality, and modulation of the microbial population both quantitatively and qualitatively. In addition, identification and understanding of the optimal method, time, rate of application and phenological stage for improving plant performance and resilience to stress as well as the best combinations of plant species/cultivar × environment × management practices are also reported. We strongly believe that high standard reflected in this compilation on the principles and practices of plant biostimulants will foster knowledge transfer among scientific communities, industries, and agronomists, and will enable a better understanding of the mode of action and application procedures of biostimulants in different cropping systems.

Primer and Language Lessons in English and Cree

Primer and Language Lessons in English and Cree PDF Author: Ervin Bird Glass
Publisher: W. Briggs ; Montreal : C.W. Coates ; Halifax, N.S. : S.F. Huestis
ISBN:
Category : Cree language
Languages : en
Pages : 122

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Book Description


Monitoring Biodiversity

Monitoring Biodiversity PDF Author: Alfonso Alonso
Publisher: Smithsonian Institution
ISBN: 1935623397
Category : Science
Languages : en
Pages : 769

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Book Description
The book compiles case studies regarding the biodiversity research and monitoring program of Andean species and habitats carefully chosen as indicators to assess the short- and long-term effects of a linear disturbance: the PERU LNG pipeline. Set in a scientifically unexplored region of the Andes, Monitoring Biodiversity clearly articulates the Smithsonian-led conceptual framework for the implementation in the field by scientists. It addresses scientific and conservation questions addressed by the research protocols, the experimental design, and data gathering. Moreover, the book covers a gap on how to integrate biodiversity research, monitoring, and conservation into sustainable development projects of national and international interest. The text is presented in both English and Spanish.

Graftless Solutions for the Edentulous Patient

Graftless Solutions for the Edentulous Patient PDF Author: Saj Jivraj
Publisher: Springer Nature
ISBN: 3031328477
Category : Medical
Languages : en
Pages : 560

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Book Description
This book, designed to meet the needs of clinicians and now in an extensively revised second edition, clearly explains the rationale and technique for the rehabilitation of fully edentulous patients utilizing traditional graftless concepts as well as zygomatic implant strategies when posterior support cannot be achieved by the former means. Considerations relevant to treatment planning and the biomechanics of immediate loading and zygomatic implants are first discussed. The techniques for placement of traditional tilted and zygomatic implants and for immediate loading of a full arch restoration are then described step by step. Detailed information and guidance are also provided on the different materials available for full arch restorations, laboratory aspects of the definitive restoration, maintenance of restorations, and management of prosthetic and surgical complications. The book concludes with a helpful series of clinical cases. Graftless Solutions for the Edentulous Patient is designed particularly for clinicians with experience in placing and restoring dental implants.