Single Nucleotide Polymorphisms PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Single Nucleotide Polymorphisms PDF full book. Access full book title Single Nucleotide Polymorphisms by Pui-Yan Kwok. Download full books in PDF and EPUB format.
Author: Pui-Yan Kwok
Publisher: Springer Science & Business Media
ISBN: 1592593275
Category : Medical
Languages : en
Pages : 276
Get Book Here
Book Description
A comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Author: Pui-Yan Kwok
Publisher: Springer Science & Business Media
ISBN: 1592593275
Category : Medical
Languages : en
Pages : 276
Get Book Here
Book Description
A comprehensive collection of readily reproducible techniques for the difficult process of single nucleotide polymorphisms (SNP) discovery and genotyping. These cutting-edge protocols for mutation/SNP detection utilize denaturing high-performance liquid chromatography (dHPLC), single-strand conformation polymorphism (SSCP), conformation-sensitive gel electrophoresis (CSGE), chemical cleavage, and direct sequencing. Equally powerful and up-to-date methods are given for genotyping SNPs, including molecular beacons, the Taqman assay, single-base extension approaches, pyrosequencing, ligation, the Invader assay, and primer extension with mass spectrometry detection.
Author: Harry Iland
Publisher: Springer Science & Business Media
ISBN: 1597450170
Category : Medical
Languages : en
Pages : 308
Get Book Here
Book Description
A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.
Author: G. Tripathi
Publisher: I. K. International Pvt Ltd
ISBN: 818823785X
Category : Medical
Languages : en
Pages : 1388
Get Book Here
Book Description
The fundamental aim underlying Cellular and Biochemical Sciences is to emphasize diversified topics of current interest to postgraduate students pursuing different courses in the area of biological sciences including Zoology, Botany, Biochemistry and Biotechnology. The text is also relevant to the students of Life Sciences, Biosciences, Cell Biology, Bioengineering and Pharmacology. A total of 58 topics have been incorporated in the book and some of the topics are rarely found in other books of Biology. New information has been introduced which updates existing knowledge and enables the book to justify its claim as the most comprehensive text in the sphere of cellular and biochemical sciences at the postgraduate and competitive examination levels. Each and every chapter has been designed in lucid and readable manner. There are references, suggested readings, long questions and objective questions at the end of chapters for revision of topics.
Author: Kirstin J. Edwards
Publisher: Taylor & Francis
ISBN: 113418400X
Category : Polymerase chain reaction
Languages : en
Pages : 362
Get Book Here
Book Description
Author: Francois Ferre
Publisher: Springer Science & Business Media
ISBN: 1461241642
Category : Medical
Languages : en
Pages : 379
Get Book Here
Book Description
Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Author: Paul M. Lieberman
Publisher: Springer Science & Business Media
ISBN: 159259848X
Category : Medical
Languages : en
Pages : 486
Get Book Here
Book Description
A compendium of readily reproducible and novel methods to manipulate DNA viruses and characterize their varied biological properties. The authors emphasize techniques for viral detection and genetics, but also include methods for structure determination, gene expression, replication, pathogenesis, complex cellular models, recombinant genetics, and computational/systems approaches. Wide-ranging and highly practical, DNA Viruses: Methods and Protocols will stimulate new directions in virology research with its novel strategies for engineering viral vectors in gene therapy, and its advanced approaches for detecting viruses in human disease.
Author: Ezio Rosato
Publisher: Springer Science & Business Media
ISBN: 1597452572
Category : Science
Languages : en
Pages : 557
Get Book Here
Book Description
This is an in-depth examination of circadian biology, presented by leading researchers in the field. Methods for analysis of rhythmic readouts in select model organisms are included. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection.
Author: M Tevfik Dorak
Publisher: Garland Science
ISBN: 0203967313
Category : Science
Languages : en
Pages : 333
Get Book Here
Book Description
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.
Author: Jang B. Rampal
Publisher: Springer Science & Business Media
ISBN: 1597453048
Category : Science
Languages : en
Pages : 927
Get Book Here
Book Description
This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.
Author:
Publisher:
ISBN:
Category : Environmental health
Languages : en
Pages : 1576
Get Book Here
Book Description
