Practical Electrophysiological Methods PDF Download
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Author: Helmut Kettenmann
Publisher: Wiley-Liss
ISBN:
Category : Medical
Languages : en
Pages : 480
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Book Description
Designed to enable newcomers to electrophysiology to choose appropriate preparations and techniques for in vitro studies in vertebrate neurobiology. Emphasizes techniques involving the glass micropipette and other recently developed methods, supplementing easy-to-follow instructions with illustrative schemes, examples of original records, photomicrographs, key word listings, comprehensive reference tables and a list of suppliers and key equipment and reagents.
Author: Helmut Kettenmann
Publisher: Wiley-Liss
ISBN:
Category : Medical
Languages : en
Pages : 480
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Book Description
Designed to enable newcomers to electrophysiology to choose appropriate preparations and techniques for in vitro studies in vertebrate neurobiology. Emphasizes techniques involving the glass micropipette and other recently developed methods, supplementing easy-to-follow instructions with illustrative schemes, examples of original records, photomicrographs, key word listings, comprehensive reference tables and a list of suppliers and key equipment and reagents.
Author: Franklin Bretschneider
Publisher: Academic Press
ISBN: 0128142111
Category : Medical
Languages : en
Pages : 372
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Book Description
Introduction to Electrophysiological Methods and Instrumentation, Second Edition covers all topics of interest to electrophysiologists, neuroscientists and neurophysiologists, from the reliable penetration of cells and the behavior and function of the equipment, to the mathematical tools available for analyzing data. It discusses the pros and cons of techniques and methods used in electrophysiology and how to avoid pitfalls. Although the basics of electrophysiological techniques remain the principal purpose of this second edition, it now integrates several current developments, including, amongst others, automated recording for high throughput screening and multimodal recordings to correlate electrical activity with other physiological parameters collected by optical means. This book provides the electrophysiologist with the tools needed to understand his or her equipment and how to acquire and analyze low-voltage biological signals. - Introduces possibilities and solutions, along with the problems, pitfalls, and artefacts of equipment and electrodes - Discusses the particulars of recording from brain tissue slices, oocytes and planar bilayers - Describes optical methods pertinent to electrophysiological practice - Presents the fundamentals of signal processing of analogue signals, spike trains and single channel recordings, along with procedures for signal recording and processing - Includes appendices on electrical safety and foundations of useful mathematical tools
Author: Ellen Covey
Publisher: Oxford University Press
ISBN: 0199342547
Category : Medical
Languages : en
Pages : 241
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Book Description
Basic Electrophysiological Methods provides a concise and easy-to-read guide on a selection of the most important contemporary electrophysiological techniques, their implementation, applications, and ways in which they can be combined and integrated with neuroscientific techniques. Intended for students, postdocs, and faculty with a basic neuroscience background, this text will not obscure the relevant technical details with textbook neuroscience tutorials as many other books do. Instead, each chapter provides a conscientious overview of the underlying theory -- a comprehensive description of equipment, materials, methods, data management, and analysis -- a troubleshooting guide, and a list of frequently asked questions. No book or online resource can function as strictly a DIY set of instructions on how to implement a complex technique. However, this book provides a fundamental and accessible set of information intended to form a foundation prior to, during, and after hands-on experience and training, greatly facilitating the initial learning process and subsequent fine-tuning of technical details.
Author: Stefan Dhein
Publisher: Springer
ISBN: 3540265740
Category : Medical
Languages : en
Pages : 1011
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Book Description
Scientists working or planning to work in the field of cardiovascular research will welcome Methods in Cardiovascular Research as the reference book they have been waiting for. Not only general aspects of cardiovascular research are well presented but also detailed descriptions of methods, protocols and practical examples. Written by leading scientists in their field, chapters cover classical methods such as the Langendorff heart or working heart models as well as numerous new techniques and methods. Newcomers and experienced researchers alike will benefit from the troubleshooting guide in each chapter, the extensive reference lists for advanced reading and the great practical experience of the authors. Methods in Cardiovascular Research is a "must have" for anybody with an interest in cardiovascular research.
Author: Daniel C. Sigg
Publisher: Springer Science & Business Media
ISBN: 1441966587
Category : Medical
Languages : en
Pages : 490
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Book Description
Cardiovascular disease is the major cause of mortality and morbidity in the Western Hemisphere. While significant progress has been made in treating a major sub-category of cardiac disease, arrhythmias, significant unmet needs remain. In particular, every day, thousands of patients die because of arrhythmias in the US alone, and atrial fibrillation is the most common arrhythmia affecting millions of patients in the US alone at a given time. Therefore, there is a public need to continue to develop new and better therapies for arrhythmias. Accordingly, an ever increasing number of biomedical, pharmaceutical, and medical personnel is interested in studying various aspects of arrhythmias at a basic, translational, and applied level, both in industry (ie Biotech, Pharmaceutical and device), and in academia. Not only has our overall understanding of molecular bases of disease dramatically increased, but so has the number of available and emerging molecular, pharmacological or device treatment based therapies. This practical, state-of-the art handbook will summarize and review key research methods and protocols, their advantages and pitfalls, with a focus on practical implementation, and collaborative cross-functional research. The volume will include visual and easy-to-use graphics, bulleted summaries, boxed summary paragraphs, links to reference websites, equipment manufacturers where appropriate, photographs of typical experimental setups and so forth, to keep this book very focused on practical methods and implementation, and yet, provide enough theory that the principles are clearly understood and can be easily applied.
Author: John R. Heckenlively
Publisher: MIT Press
ISBN: 0262083469
Category : Medical
Languages : en
Pages : 1033
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Book Description
The long-awaited second edition of an authoritative reference on electrophysiologic vision testing, including detailed information on techniques and problems, basic physiology and anatomy, theoretical concepts, and clinical findings; with extensive new material. This authoritative text is the only comprehensive reference available on electrophysiologic vision testing, offering both practical information on techniques and problems as well as basic physiology and anatomy, theoretical concepts, and clinical correlations. The second edition, of the widely used text, offers extensive new material and updated information: 65 of the 84 chapters are completely new, with the changes reflecting recent advances in the field. The book will continue to be an essential resource for practitioners and scholars from a range of disciplines within vision science. The contributions not only cover new information—important material that is likely to become more important in the next decade—but also offer a long-range perspective on the field and its remarkable development in the last century. After discussing the history and background of clinical electrophysiology, the book introduces the anatomy of the retina and principles of cell biology in the visual pathways at the molecular, physiological, and biochemical levels. It relates these new findings to the techniques and interpretations of clinical tests, including the electro-oculogram (EOG), electroretinogram (ERG), and visual evoked potentials (VEP), which are discussed in detail, as are equipment, data acquisition and analysis, principles and protocols for clinical testing, diseases and dysfunction, and animal testing. Notable additions for this edition include chapters on the origin of electroretinogram waveforms, multifocal techniques, testing in standard laboratory animals, recent advances in analysis of abnormalities in disease, and the applications of these techniques to the study of genetic abnormalities.
Author: Nicholas Graziane
Publisher: Humana
ISBN: 9781071625910
Category : Medical
Languages : en
Pages : 0
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Book Description
This second edition volume expands on the previous edition with discussions on the latest techniques used to study synaptic transmissions. The chapters in this book are organized into six parts. Part One looks at the basic concepts, such as extracellular and intracellular recordings, and spatiotemporal effects of synaptic currents. Part Two describes the recording of synaptic currents, such as measuring kinetics of synaptic current and measuring reversal potentials. Part Three discusses basic experimentations of synaptic transmission and covers run-up and run-down, and amplitude. Parts Four and Five cover experimentations with computational components and molecular and visual components, such as measurement of a single synapse and electrophysiological and visual tags. Part Six explores in vivo recordings and talks about general considerations for in vivo exploration of synaptic plasticity. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and thorough, Electrophysiological Analysis of Synaptic Transmission, Second Edition is a valuable resource that introduces graduate students and postdoctoral fellows to important topics in this field and also expands these topics to practical electrophysiological approaches.
Author: George J. Klein MD
Publisher: Cardiotext Publishing
ISBN: 1935395157
Category : Medical
Languages : en
Pages : 211
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Book Description
From senior electrophysiologist and world-class educator George Klein, a fully illustrated guide with over 100 intracardiac tracings and figures that allow the physician to approach electrophysiologic problems effectively and systematically. The book is especially focused on electrophysiological maneuvers and provides a clear and understandable guide to their proper selection and interpretation using abundant clinical examples. Defines the integral role for "traditional" electrogram (EGM) analysis in order to understand the mechanism of a tachycardia. It goes without saying that a correct arrhythmia diagnosis is a prerequisite to catheter ablation regardless of the presence of sophisticated mapping and imaging technologies. Electrophysiological maneuvers are fundamental to this process, and proper selection and interpretation of maneuvers constitute a core skill of the electrophysiologist. In this volume, we make the case that most maneuvers are fundamentally similar in principle and can be understood by appreciating a few basic physiological and anatomical principles. The art lies not in a comprehensive knowledge by rote of every maneuver or its application, but rather a systematic approach using common principles. We illustrate this by showing abundant examples and emphasizing the "game plan," including checklists that can be applied to virtually any maneuver. —George J. Klein In my opinion, this book should be on the shelf of every electrophysiologist trainee as well as every clinical cardiac electrophysiologist. It is a classic, like its editor. Dr. Klein deserves high praise for organizing his and his colleagues’ clinical experiences and thought processes into a concise, practical text that should be part of all training programs in electrophysiology. —From the foreword by Mark E. Josephson, MD
Author: John Dempster
Publisher:
ISBN: 9780122089404
Category : Medical
Languages : en
Pages : 228
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Book Description
A Volume in the Biological Techniques Series. This essential bench-top manual is the first comprehensive introduction to the principles and practice of the analysis of electrophysiological signals. The text describes hardware and software, including specific techniques for the analysis of single ion channels, noise analysis, and voltage-clamped whole cell currents. Rather than simply providing a manual describing how to use computer programs, this book provides insight into why programs operate in particular ways, the pitfalls awaiting the unwary, and the general philosophy behind signal analysis techniques. Examples of program source code are included. Key Features * Explains the principles of the digitization of analog signals * Provides practical examples for hardware in common use in the electrophysiological laboratory
Author: Areles Molleman
Publisher: John Wiley & Sons
ISBN: 0470856513
Category : Science
Languages : en
Pages : 186
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Book Description
Patch clamping is a widely applied electrophysiological technique for the study of ion channels; membrane proteins that regulate the flow of ions across cellular membranes and therefore influence the physiology of all cells. Patch Clamping aims to cover the basic principles and practical applications of this important technique. Starting with a review of the history of patch clamping, the text then goes on to cover the basic principles, platforms, equipment and environmental control, and will also include coverage of preparation types, recording modes and analysis of results. This book will explain the basic principles and practical application of patch clamp electrophysiology Written in a non-technical style to ensure its broad appeal to novice users Takes a practical approach This self-contained guide provides everything a practising patch clamp electrophysiologist needs to know to master this technique, including an overview of membrane biophysics, standard experimental design, data analysis, and technical concerns
