Polyamine Protocols

Polyamine Protocols PDF Author: David M. L. Morgan
Publisher: Springer Science & Business Media
ISBN: 1592595650
Category : Science
Languages : en
Pages : 173

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Book Description
A unique collection of hands-on enzyme assay techniques to study polyamines and their function. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. The methods are presented by leading researchers who have perfected them to a high art, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Polyamine Protocols

Polyamine Protocols PDF Author: David M. L. Morgan
Publisher: Springer Science & Business Media
ISBN: 1592595650
Category : Science
Languages : en
Pages : 173

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Book Description
A unique collection of hands-on enzyme assay techniques to study polyamines and their function. The techniques range from assay methods for enzymes of polyamine biosynthesis and catabolism to measurements of polyamines, polyamine transport, and polyamine effects on cell growth. The methods are presented by leading researchers who have perfected them to a high art, and include clear, step-by-step instructions with numerous hints and tips to ensure readily reproducible results.

Polyamines

Polyamines PDF Author: Anthony E. Pegg
Publisher: Humana Press
ISBN: 9781617790355
Category : Science
Languages : en
Pages : 524

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Book Description
Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development. In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study. The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.

Polyamines

Polyamines PDF Author: Kazutomo Imahori
Publisher: VSP
ISBN: 9789067640428
Category : Science
Languages : en
Pages : 548

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Book Description
The significance of polyamines in cell proliferation and carcinogenesis has stimulated further research interest in their biochemistry and metabolism. These papers, which update the available literature on polyamines, are grouped into four main areas: the metabolism of polyamines; the effect of polyamines on cellular metabolism; inhibitors of polyamine metabolism and their clinical relevance; application to diagnosis and prognosis. The Proceedings include contributions from a wide range of research interests with special emphasis being placed on the clinical aspects of these compounds.

Polyamines in Health and Nutrition

Polyamines in Health and Nutrition PDF Author: Susan Bardocz
Publisher: Springer Science & Business Media
ISBN: 9780412822209
Category : Science
Languages : en
Pages : 360

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Book Description
The importance of polyamines for all living cells has been recognized since spermine was discovered in human semen more than 300 years ago. Polyamine research intensified when analytical methods were developed for their determination, particularly in tissues and biological fluids. Discovering their close correlation with cancer, and that polyamine concentrations change during the cell cycle, gave reason for further research in this topic. Polyamines in Health and Nutrition concentrates on the direction of polyamine research which has the capacity to influence and benefit our health and which can explain some of the discrepancies and failures of earlier research. It is important to recognize the dietary contribution to the polyamine body pool and to investigate how the polyamine content of the diet can be changed, with the ultimate aim of using this information to improve our health.

Approaches to Design and Synthesis of Antiparasitic Drugs

Approaches to Design and Synthesis of Antiparasitic Drugs PDF Author: N. Anand
Publisher: Elsevier
ISBN: 0080527523
Category : Medical
Languages : en
Pages : 525

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Book Description
This book presents a comprehensive and up to date account of the chemotherapy of parasitic diseases, both human and veterinary. The book starts with an overview of parasitic diseases. The body of the book is divided into two parts: antihelminthic drugs, and antiprotozoal drugs. Both parts start with chapters highlighting the 'biochemical targets' available for chemotherapeutic interference. Individual chapters deal with one chemical class of compounds and describe their origin, structure-activity relationship, mode of action, and methods of synthesis and their status both in clinical and veterinary practice. The book will be useful to a wide spectrum of readers: students embarking on a research career in parasitic chemotherapy, clinicians (and veterinarians) and clinical pharmacologists desiring detailed information about the drugs currently in use, and pharmaceutical technologists wanting to update their knowledge of the methods of manufacture.

Comprehensive Supramolecular Chemistry II

Comprehensive Supramolecular Chemistry II PDF Author: George W. Gokel
Publisher: Elsevier
ISBN: 0128031999
Category : Science
Languages : en
Pages : 4627

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Book Description
Comprehensive Supramolecular Chemistry II, Second Edition, Nine Volume Set is a ‘one-stop shop’ that covers supramolecular chemistry, a field that originated from the work of researchers in organic, inorganic and physical chemistry, with some biological influence. The original edition was structured to reflect, in part, the origin of the field. However, in the past two decades, the field has changed a great deal as reflected in this new work that covers the general principles of supramolecular chemistry and molecular recognition, experimental and computational methods in supramolecular chemistry, supramolecular receptors, dynamic supramolecular chemistry, supramolecular engineering, crystallographic (engineered) assemblies, sensors, imaging agents, devices and the latest in nanotechnology. Each section begins with an introduction by an expert in the field, who offers an initial perspective on the development of the field. Each article begins with outlining basic concepts before moving on to more advanced material. Contains content that begins with the basics before moving on to more complex concepts, making it suitable for advanced undergraduates as well as academic researchers Focuses on application of the theory in practice, with particular focus on areas that have gained increasing importance in the 21st century, including nanomedicine, nanotechnology and medicinal chemistry Fully rewritten to make a completely up-to-date reference work that covers all the major advances that have taken place since the First Edition published in 1996

Flavoprotein Protocols

Flavoprotein Protocols PDF Author: Steven K. Chapman
Publisher: Springer Science & Business Media
ISBN: 159259266X
Category : Science
Languages : en
Pages : 257

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Book Description
As a scientist with an interest in proteins you will, at some time in your career, isolate an enzyme that turns out to be yellow—or perhaps you already have. Alternatively, you may identify a polypeptide sequence that is related to known flavin-containing proteins. This may, or may not, be your first encounter with flavoproteins. However, even if you are an old hand in the field, you may not have exploited the full range of experimental approaches applicable to the study of flavoproteins. We hope that Flavoprotein Protocols will encourage you to do so. In this volume we have sought to bring together a range of experimental methods of value to researchers with an interest in flavoproteins, whether or not these researchers have experience in this area. A broad range of techniques, from the everyday to the more specialized, is described by scientists who are experts in their fields and who have ext- sive practical experience with flavoproteins. The wide range of approaches, from wet chemistry to dry computation, has, as a consequence, demanded a range of formats. Where appropriate (particularly for analytical methods) the protocol described is laid out in easy-to-follow steps. In other cases (e. g. , the more advanced spectroscopies and computational methods) it is far more apt to describe the general approach and relevance of the methods. We hope this wide-ranging approach will sow the seeds of many future collaborations - tween laboratories and further our knowledge and understanding of how f- voproteins work.

The Handbook of Microbial Metabolism of Amino Acids

The Handbook of Microbial Metabolism of Amino Acids PDF Author: J P F D'Mello
Publisher: CABI
ISBN: 1780647239
Category : Science
Languages : en
Pages : 541

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Book Description
This book collates and reviews recent advances in the microbial metabolism of amino acids, emphasizing diversity - in terms of the range of organisms under investigation and their natural ecology - and the unique features of amino acid metabolism in bacteria, yeasts, fungi, protozoa and nematodes. As well as studying the individual amino acids, including arginine, sulfur amino acids, branched-chain amino acids and aromatic amino acids, a number of themes are explored throughout the work. As the volume of research into the metabolism of amino acids grows, this comprehensive study of the subject is a vital tool for researchers in the fields of biological, medical and veterinary sciences, including microbiology, biochemistry, genetics and pathology. This book is also essential for corporate organizations with active research and development programmes, such as those in the pharmaceutical industry.

Practical Cell Analysis

Practical Cell Analysis PDF Author: Dimitri Pappas
Publisher: John Wiley & Sons
ISBN: 9780470688458
Category : Science
Languages : en
Pages : 314

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Book Description
As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be “common knowledge” to one field but are often hidden or unknown to another. Within this practical guide: The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented. Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells Many diagram-based protocols for some of the more common cell processes are included Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.

Inhibition of Polyamine Metabolism

Inhibition of Polyamine Metabolism PDF Author: Peter McCann
Publisher: Elsevier
ISBN: 0323152325
Category : Medical
Languages : en
Pages : 388

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Book Description
Inhibition of Polyamine Metabolism: Biological Significance and Basis for New Therapies provides an overview of the field concerning polyamine biosynthesis inhibitors. The book is comprised of chapters which describe the compounds which are in use; the rationale for the design of such inhibitors; their synthesis and action; their biological effects on mammalian cells and tissues, plants, and microorganisms including protozoal parasites; and a review of the clinical experience with these inhibitors. The major beneficial effect which the wide availability of these inhibitors has had on the field of polyamine research in general is likewise demonstrated. Biochemists, pharmacologist, and cellular physiologists will find this text very useful.