Pollen Cryopreservation Protocols PDF Download
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Author: P.E. Rajasekharan
Publisher: Springer Nature
ISBN: 1071628437
Category : Science
Languages : en
Pages : 605
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Book Description
This volume focuses upon pollen cryopreservation in various crops from the most widely cultivated tomato to the lesser known medicinal species like Decalepis arayalpatra. Chapters detail standard techniques such as pollen collection, cryopreservation, pollen , germination tests for viability, and specific crops. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs. Authoritative and cutting-edge, Pollen Cryopreservation Protocols aims to serve as reference for researchers studying plant breeding and plant conservation biology.
Author: P.E. Rajasekharan
Publisher: Springer Nature
ISBN: 1071628437
Category : Science
Languages : en
Pages : 605
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Book Description
This volume focuses upon pollen cryopreservation in various crops from the most widely cultivated tomato to the lesser known medicinal species like Decalepis arayalpatra. Chapters detail standard techniques such as pollen collection, cryopreservation, pollen , germination tests for viability, and specific crops. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs. Authoritative and cutting-edge, Pollen Cryopreservation Protocols aims to serve as reference for researchers studying plant breeding and plant conservation biology.
Author: Barbara B.M. Reed
Publisher: Springer Science & Business Media
ISBN: 0387722769
Category : Technology & Engineering
Languages : en
Pages : 534
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Book Description
Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Author: John G. Day
Publisher: Springer Science & Business Media
ISBN: 1588293777
Category : Science
Languages : en
Pages : 703
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Book Description
In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.
Author: Víctor M. Loyola-Vargas
Publisher: Humana Press
ISBN: 9781617798177
Category : Science
Languages : en
Pages : 430
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Book Description
Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. “Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.”Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.
Author: Y. P. S. Bajaj
Publisher: Springer Science & Business Media
ISBN: 3662030969
Category : Technology & Engineering
Languages : en
Pages : 526
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Book Description
The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Author: L.E. Towill
Publisher: Springer Science & Business Media
ISBN: 3662046741
Category : Science
Languages : en
Pages : 406
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Book Description
Ex situ preservation of germplasm for higher plant species has been accom plished using either seeds or clones, but storage of these under typical condi tions does not provide the extreme longevities that are needed to minimize risk of loss. Costs of maintenance and regeneration of stocks are also high. Systems that provide virtually indefinite storage should supplement existing methods and it is within this context that cryopreservation is presented. The use of low temperature preservation was initially more a concern of medicine and animal breeding, and was expanded to plants in the 1970s. Sur vival after cryogenic exposure has now been demonstrated for diverse plant groups including algae, bryophytes, fungi and higher plants. If survival is com monplace, then the eventual application is a cryopreservation system, whereby cells, tissues and organs are held indefinitely for use, often in the unforeseen future. The increasing interest and capabilities for application could not have occurred at a more opportune time since expanding human populations have placed unprecedented pressures on plant diversity. This book emphasizes cry opreservation of higher plants and was initially driven by the concern for loss of diversity in crops and the recognized need that this diversity would be essential for continued improvement of the many plants used by society for food, health and shelter. The interest in cryopreservation has been expanded by conservationists and their concerns for retaining, as much as possible, the diversity of natural populations. The need for cryopreservation, thus, is well established.
Author: Saikat Gantait
Publisher: Springer Nature
ISBN: 9819714672
Category :
Languages : en
Pages : 683
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Book Description
Author: Jacques Donnez
Publisher: Cambridge University Press
ISBN: 1139496123
Category : Medical
Languages : en
Pages : 569
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Book Description
The specialty of fertility preservation offers patients with cancer, who are rendered infertile by chemo- and radiotherapy, the opportunity to realize their reproductive potential. This gold-standard publication defines the specialty. The full range of techniques and scientific concepts is covered in detail, and the author team includes many of the world's leading experts in the field. The book opens with introductions to fertility preservation in both cancer and non-cancer patients, followed by cancer biology, epidemiology and treatment, and reproductive biology and cryobiology. Subsequent sections cover fertility preservation strategies in males and females, including medical/surgical procedures, ART, cryopreservation and transplantation of both ovarian tissue and the whole ovary, and in-vitro follicle growth and maturation. Concluding chapters address future technologies, as well as ethical, legal and religious issues. Richly illustrated throughout, this is a key resource for all clinicians specializing in reproductive medicine, gynecology, oncology, hematology, endocrinology and infertility.
Author: P.E. Rajasekharan
Publisher: Springer
ISBN: 9811336695
Category : Technology & Engineering
Languages : en
Pages : 684
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Book Description
The conservation of crop genetic resources is one of the important elements in efforts to sustainably increase agricultural production in low-income countries, and to guarantee long-term food security, especially for the low-income population groups in these countries. Horticultural crops, as high-value crops, have an important role to play in revitalizing rural economies and can add significantly to national economies. Moreover, horticulture provides more than twice the number of jobs compared to traditional cereal crop production, and the shifting of conventional agriculture towards high-value horticulture has increased employment opportunities in developing countries. To exploit this potential, researchers need a vast array of horticultural genetic resources and information on new traits. Horticultural crops, which are only a part of PGRFA (Plant Genetic Resources for Food and Agriculture), are characterized by a wide and varied range of species. In fact, there are five major horticultural crop groups: fruit and nut crops, vegetables, food legumes, roots and tubers, and lastly the ornamental and medicinal group. In this context, the present book provides a comprehensive overview of the current state of conservation and utilization of horticultural genetic resources, addressing contemporary approaches to conservation in connection with different technologies, including biotechnological approaches as practised in India and in some cases, globally. It includes a brief chapter on the unique nature of horticultural genetic resources, providing a rationale for viewing them as being distinct from field crop genetic resources. Subsequent chapters share insights on protocols for the conservation of selected horticultural crops ex situ, and focus on the increased need to complement these efforts with in situ conservation approaches. Geospatial tools are also briefly described, emphasizing their utility with regard to mapping and managing resources. The book also explores the wild gene pool in horticulture crops; discusses legal aspects related to horticultural genetic resources and biotechnological aspects; and describes the key aspects of sustainable management and replenishment. Given its scope, the book offers a valuable resource for all horticulturists, graduate students, researchers, policymakers, conservationists, and NGOs engaged in horticulture in particular and biodiversity in general.
Author: T. D. Hong
Publisher: Bioversity International
ISBN: 9290432799
Category : Seeds
Languages : en
Pages : 62
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Book Description
This publication provides an approach by which conservationists can determine whether or not long-term seed storage is feasible for a particular species, i.e. whether or not that species shows orthodox seed storage behaviour, and provides advice on the implementation of the protocol, examples of ways in which the results from seed storage studies could be misinterpreted due to confounding factors, as well as several alternative approaches for estimating seed storage behaviour prior to carryng out actual investigations with the seeds. In particular, the latter section introduces the concept of a multicriteria approach for estimating seed storage behaviour.
