Author: Franck Pellestor
Publisher: Nova Publishers
ISBN: 9781600214134
Category : Science
Languages : en
Pages : 208

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Book Description
Book & CD. Advances in molecular biotechnology have greatly improved the sensitivity and the efficiency of methods utilised for genetic investigations and diagnosis. In the domain of chromosome analysis, the introduction of molecular techniques has led to the development of a new approach, called Molecular Cytogenetics, which has surpassed previously available techniques to become a foremost biological method. The fluorescence in situ hybridisation (FISH) is quickly became the standard technique for in situ chromosomal investigations, as illustrated by its large variety of applications in research and diagnosis. However, during the last decade, alternative methods to FISH have been introduced and have shown to be valuable in detecting chromosomes and quantifying chromosomal abnormalities. These alternative procedures are the Primed IN Situ (PRINS) labelling and the Peptide Nucleic Acid (PNA) probes. The two procedures present several advantages for the in situ detection of nucleic acid sequences, such as the small size of PNA probes and PRINS primers, or the fast kinetics of PRINS and PNA labelling reactions, that make them very attractive for a number of cytogenetic purposes. This book provides a valuable introduction and overview of the principles and the applications of alternative approaches in the field of molecular cytogenetics.

Author: William C. Heiser
Publisher: Springer Science & Business Media
ISBN: 1592596495
Category : Science
Languages : en
Pages : 303

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Book Description
Experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1 focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. Volume 2 details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Author:
Publisher:
ISBN:
Category : Environmental health
Languages : en
Pages : 636

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Book Description

Author: E. D. Adams
Publisher:
ISBN:
Category : Soil surveys
Languages : en
Pages : 130

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Author:
Publisher:
ISBN:
Category : Biochemical markers
Languages : en
Pages : 288

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Book Description

Author: Frank L Anderson
Publisher:
ISBN:
Category : Soil surveys
Languages : en
Pages : 182

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Book Description

Author: Robert Snyder
Publisher:
ISBN:
Category : Air
Languages : en
Pages : 804

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Book Description

Author:
Publisher:
ISBN:
Category : Soil surveys
Languages : en
Pages : 808

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Book Description

Author: Floyd V. Brent
Publisher:
ISBN:
Category : Soil surveys
Languages : en
Pages : 144

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Book Description

Author: Christopher Janson
Publisher: Springer Science & Business Media
ISBN: 9780306482304
Category : Medical
Languages : en
Pages : 300

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Book Description
This volume is unique to the existing literature in the Peptide Nucleic Acid field, in that it focuses on comparing and contrasting PNA with other available oligonucleotide homologues and considers areas in which these biomolecules could be profitably applied to clinical and diagnostic applications. Part I of the book addresses comparative strengths and weaknesses of various nucleoside homologues. Part II of the book addresses specific translational or clinical applications for PNA and related antisense biomolecules. The editors have succeeded in presenting a balanced yet broad view of the methods available for gene targeting and modification.