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Author: Marie-Angèle Grandbastien
Publisher: Springer Science & Business Media
ISBN: 3642318428
Category : Science
Languages : en
Pages : 338
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Book Description
Transposable elements are short lengths of DNA with the capacity to move between different points within a genome. This process can affect the function of genes at or near the insertion site. The present book gives an overview of the impact of transposable elements on plant genomes and explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies like “new generation sequencing.” Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail, and also illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that they are not just “junk DNA.”
Author: Marie-Angèle Grandbastien
Publisher: Springer Science & Business Media
ISBN: 3642318428
Category : Science
Languages : en
Pages : 338
Get Book
Book Description
Transposable elements are short lengths of DNA with the capacity to move between different points within a genome. This process can affect the function of genes at or near the insertion site. The present book gives an overview of the impact of transposable elements on plant genomes and explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies like “new generation sequencing.” Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail, and also illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that they are not just “junk DNA.”
Author: Jungnam Cho
Publisher: Humana
ISBN: 9781071611364
Category : Science
Languages : en
Pages : 272
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Book Description
This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.
Author: Deepu Pandita
Publisher:
ISBN: 9781774911822
Category : Plant biotechnology
Languages : en
Pages : 0
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Book Description
"This new volume provides an up-to-date understanding of the numerous classes of plant transposable elements, the mobile units of DNA that comprise large portions of plant genomes, an important contributor for gene and genome evolution. Transposable elements (TEs) are major components of large plant genomes and main drivers of genome evolution, known to produce a wide variety of changes in plant gene expression and function. This book, Plant Transposable Elements: Biology and Biotechnology, provides a systematic interpretation of protocols designed to characterize TEs and their biotechnological roles. The chapters explore the role of TEs in plant development, their architecture, their epigenetic regulation, their use in DNA repair, their evolution and speciation, while highlighting their importance in the approaching epoch of climate change. It discusses the applications of the transposons in genome editing and their biology, classification, structure, functions, and evolution. The volume begins with introduction of transposable elements (TEs), covering their introduction, classification, and transposition. It delves into protocols designed to characterize TEs and their biotechnological applications. This section looks at computational approaches for prediction and analysis, retro-transposon capture sequencing, and more. The section on transposon biology focuses on its role in plant development and as natural genetic engineers of genome mutation, evolution, and speciation. The book looks further into transposon applications in genome editing, exploring tagging and mutagenesis, genome engineering, and more. The last chapter uses the example of Oryza sativa to elucidate on the classification, structure, function, and evolution of transposable elements. This comprehensive volume is a valuable comprehensive resource for researchers, faculty, and students in biology, biotechnology, genetics, and botany and plant science."--
Author: O. Nelson
Publisher: Springer Science & Business Media
ISBN: 1468455508
Category : Medical
Languages : en
Pages : 396
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Book Description
Transposon tagging can work. Even though most of our understand ing about the factors that contribute to a successful tagging experiment has been accumulated from a limited number of experiments using different transposable elements in different genetic backgrounds, it is still possible to draw some conclusions regarding the best experimental strategies for gene tagging. In our experience, Spm has proved to be a good element for transposon tagging. The frequency of recovering mutable alleles in duced by Spm is not significantly different from that for Ac-Ds or for Mu 6 (summarized in Ref. 22) and varies from about 10- to 10=zr:-8pm has the unique advantage, however, in that all of the members of thiSfumily that have been examined thus far are homologous to each other at the DNA level. Therefore, by combining molecular analysis with genetic segre gation, it is possible to identify and isolate alleles that are due to insertions of either autonomous or nonautonomous Spm elements. There are definite steps one can take to increase the chances of de tecting a transposition into the gene of interest. The most important step is to select a genetic background in which the desired phenotype will be easy to screen. If the phenotype is not likely to be mutable, then tester lines should be constructed so as to contain flanking markers that can aid in subsequent segregation analyses.
Author: Nina V. Fedoroff
Publisher: John Wiley & Sons
ISBN: 1118500105
Category : Science
Languages : en
Pages : 240
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Book Description
The transposable genetic elements, or transposons, as they are now known, have had a tumultuous history. Discovered in the mid-20th century by Barbara McClintock, they were initially received with puzzlement. When their genomic abundance began to be apparent, they were categorized as "junk DNA" and acquired the label of parasites. Expanding understanding of gene and genome organization has revealed the profound extent of their impact on both. Plant Transposons and Genome Dynamics in Evolution captures and distills the voluminous research literature on plant transposable elements and seeks to assemble the big picture of how transposons shape gene structure and regulation, as well as how they sculpt genomes in evolution. Individual chapters provide concise overviews of the many flavors of plant transposons and of their roles in gene creation, gene regulation, development, genome evolution, and organismal speciation, as well as of their epigenetic regulation. This volume is essential reading for anyone working in plant genetics, epigenetics, or evolutionary biology.
Author: Jonathan Wendel
Publisher: Springer Science & Business Media
ISBN: 3709111307
Category : Science
Languages : en
Pages : 282
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Book Description
In this timely new 2-volume treatise, experts from around the world have banded together to produce a first-of-its-kind synopsis of the exciting and fast moving field of plant evolutionary genomics. In Volume I of Plant Genome Diversity, an update is provided on what we have learned from plant genome sequencing projects. This is followed by more focused chapters on the various genomic “residents” of plant genomes, including transposable elements, centromeres, small RNAs, and the evolutionary dynamics of genes and non-coding sequences. Attention is drawn to advances in our understanding of plant mitochondrial and plastid genomes, as well as the significance of duplication in genic evolution and the non-independent evolution among sequences in plant genomes. Finally, Volume I provides an introduction to the vibrant new frontier of plant epigenomics, describing the current state of our knowledge and the evolutionary implications of the epigenomic landscape.
Author: Jungnam Cho
Publisher:
ISBN: 9781071611340
Category : Electronic books
Languages : en
Pages : 272
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Book Description
This volume details the most up-to-date technologies used in plant transposable element studies and provides easy-to-follow protocols. Chapters guide readers on available database resources, annotation of different families of transposon, and experimental methods to detect their transposition intermediates, neo-transposed DNAs, and transposition events. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Transposable Elements: Methods and Protocols aims to provide web-lab and dry-lab methodologies targeted at various levels from beginner to experienced.
Author: Jeffrey Bennetzen
Publisher: Springer
ISBN: 3319974270
Category : Science
Languages : en
Pages : 390
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Book Description
This book discusses advances in our understanding of the structure and function of the maize genome since publication of the original B73 reference genome in 2009, and the progress in translating this knowledge into basic biology and trait improvement. Maize is an extremely important crop, providing a large proportion of the world’s human caloric intake and animal feed, and serving as a model species for basic and applied research. The exceptionally high level of genetic diversity within maize presents opportunities and challenges in all aspects of maize genetics, from sequencing and genotyping to linking genotypes to phenotypes. Topics covered in this timely book range from (i) genome sequencing and genotyping techniques, (ii) genome features such as centromeres and epigenetic regulation, (iii) tools and resources available for trait genomics, to (iv) applications of allele mining and genomics-assisted breeding. This book is a valuable resource for researchers and students interested in maize genetics and genomics.
Author: Marie-Angèle Grandbastien
Publisher: Springer
ISBN: 9783642318436
Category : Science
Languages : en
Pages : 330
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Book Description
Transposable elements are short lengths of DNA with the capacity to move between different points within a genome. This process can affect the function of genes at or near the insertion site. The present book gives an overview of the impact of transposable elements on plant genomes and explains how to recognize and study transposable elements, e.g. by using state-of-the-art strategies like “new generation sequencing.” Moreover, the impact of transposable elements on plant genome structure and function is reviewed in detail, and also illustrated in examples and case studies. The book is intended both for readers familiar with the field and for newcomers. With large-scale sequencing becoming increasingly available, more and more people will come across transposable element sequences in their data, and this volume will hopefully help to convince them that they are not just “junk DNA.”
Author: Andrew Kalinski
Publisher: DIANE Publishing
ISBN: 9780788119897
Category : Plant molecular genetics
Languages : en
Pages : 194
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Book Description
The following terms were used in this search: plant/s, transposable element/s, and mobile element/s. Includes the following information for the sponsored projects: title, investigators, performing organization, location of performing organization, objectives, and approach. The bibliography includes 129 citations from 1984 through 1991, and 162 citations 1992 through Sep. 1994. Some citations have abstracts.
