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Author: Fowke
Publisher: CRC Press
ISBN: 1351084224
Category : Science
Languages : en
Pages : 254
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Book Description
The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.
Author: Fowke
Publisher: CRC Press
ISBN: 1351084224
Category : Science
Languages : en
Pages : 254
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Book Description
The present book thus emphasizes the importance of plant protoplasts for fundamental research. This book is intended to be used b senior undergraduates, graduate students, and research scientists in plant biology.
Author: Y. P. S. Bajaj
Publisher: Springer
ISBN:
Category : Science
Languages : en
Pages : 472
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Book Description
Author: Kan Wang
Publisher: Humana
ISBN: 9781071621639
Category : Science
Languages : en
Pages : 263
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Book Description
This detailed volume collects protocols from scientists who are actively engaged in developing or using protoplast technology. The book begins with chapters that focus on basic protoplast techniques and their utilities, such as protocols on protoplast isolation, transfection, and regeneration, as well as examples of how to use protoplasts for genome editing and gene function analysis in a number of major crop or model plant species. It continues by exploring protoplast automation, large scale functional genomics, and synthetic biology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Protoplast Technology: Methods and Protocols aims to inspire the new generation of researchers to further improve their protocols and apply this technology to accelerate the field of plant genomic study.
Author: K.J. Puite
Publisher: Springer Science & Business Media
ISBN: 9400927886
Category : Science
Languages : en
Pages : 415
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Book Description
Proceedings of the 7th International Protoplast Symposium, Wageningen, The Netherlands, December 6-11, 1987
Author: Tessa Bengochea
Publisher: Springer Science & Business Media
ISBN: 9400940955
Category : Science
Languages : en
Pages : 97
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Book Description
Isolated plant protoplasts are 'naked' cells that have had their cell wall removed either by mechanical action or by enzymic digestion. As a result of wall removal the only barrier that exists between the cell protoplasm and the external environment is the plasma membrane. The removal of the cell wall has drastic osmotic consequences for the isolated cell. Rather like animal cells, the isolated protoplasts must be maintained in an osmotically balanced (isotonic) nutrient medium or they will burst. As will be seen later, the isolation of plant protoplasts is not a particularly new event; as early as the 1890s scientists were isolating protoplasts mechanically and studying effects such as protoplast streaming. What has brought protoplast technology to the forefront of plant biology is the potential application of these structures in non-traditional plant improvement methods. Extreme care must be exercised by the reader, however, in be lieving some of the claims that have been put forward for the use of protoplasts in improvement programmes. In the 20 years since the first experiments in routine enzyme isolation of large numbers of protoplasts no real improvement has been obtained in a commercial crop plant. This does not mean that improvement is impossible; it simply indicates that it may be a longer term objective than some people would like to admit.
Author: Professor Dr. Y. P. S. Bajaj
Publisher: Springer Science & Business Media
ISBN: 3662093669
Category : Technology & Engineering
Languages : en
Pages : 432
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Book Description
In continuation of Volumes 8, 9, 22, and 23, this new volume deals with the regeneration of plants from isolated protoplasts and genetic transformation in various species of Actinidia, Allocasuarina, Anthurium, Antirrhinum, Asparagus, Beta, Brassica, Carica, Casuarina, Cyphomandra, Eucalyptus, Ipomoea, Larix, Limonium, Liriodendron, Malus, Musa, Physcomitrella, Physalis, Picea, Rosa, Tagetes, Triticum, and Ulmus. These studies reflect the far-reaching implications of protoplast technology in genetic engineering of plants. The book contains a wealth of useful information for advanced students, teachers, and researchers in the field of plant tissue culture, molecular biology, genetic engineering, plant breeding, and general biotechnology.
Author: Michael R. Davey
Publisher: John Wiley & Sons
ISBN: 9780470686515
Category : Science
Languages : en
Pages : 358
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Book Description
The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.
Author: Kenneth Giles
Publisher: Springer Science & Business Media
ISBN: 1468443798
Category : Science
Languages : en
Pages : 485
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Book Description
The current and potential importance of plant tissue culture techniques in crop improvement is hard to overemphasize. There are few areas where these techniques will have more possible im pact than in tropical agriculture, where the availability of high productivity varieties is sadly lacking in many species. The potential for the rapid, clonal propagation of elite individuals and the use of controlled multiline planting could have a major effect on crop yield and disease resistance in many areas of the world. This volume is a collection of papers presented at the Con ference on "Crop Improvement Through Tissue Culture", held at the Base Institute, Calcutta, India in December 1981. It attempts to bring together local research workers, familiar with the agri cultural resources of the area and tissue culture and molecular 4 level workers. It was the hope of the conference that the "cross fertilization" of ideas would lead to new approaches and activity in this area. The editors trust that this collection of papers will stimu~ late interest and research in the tissue culture and improvement of crop plants everywhere. v ACKNOWLEDGEMENTS The symposium from which the papers in this book are drawn was held at Bose Institute, Calcutta on December 6 to December 10, 1981.
Author: Kenneth L. Giles
Publisher: Elsevier
ISBN: 1483218392
Category : Science
Languages : en
Pages : 321
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Book Description
Plant Protoplasts covers the techniques involved with, and uses of, protoplast technology. The book discusses isolation, fusion, and culture of higher plant protoplasts, lower plant protoplasts, and blue-green algal protoplast. The text also describes the production of haploid protoplasts from developing pollen grains; the use of protoplasts in mutant selection schemes, and the development of protoplast systems for use with monocotyledonous plants. The book will be invaluable to plant technologists, botanists, biochemists, research workers as well as advanced students interested in gaining a background knowledge of the field.
Author: Paul-Emile Pilet
Publisher: Springer Science & Business Media
ISBN: 3642701442
Category : Science
Languages : en
Pages : 296
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Book Description
The idea for the p~esent book arose from a 3-day seminar which I organized in March 1984 for young research workers in plant physiology. Participants came from several universities of the French-speaking part of Switzerland and speakers from Basel, Mtinchen, Nottingham, Perpignan, Regensburg, Sheffield, Toulouse, Yale, ZUrich ... and Lausannne. The theme of the seminar was chosen from the range of research fields of our Institute. Meanwhile, feeling it was important to bear in mind that some of our hearers were not specialists in the chosen topic, I wished to pro vide a subject that would be of scientific, methodological and epistemologi cal interest. The critical analysis of the structural and functional characteristics of plant protoplasts exactly met these criteria. There exists ample material for discussion of the techniques of protoplast preparation, methods used in morphological, biological and biochemical studies, and for the comparison of protoplasts with the cells from which they are obtained.
