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Author: Edward Chee Tak Yeung
Publisher: Springer
ISBN: 3319199447
Category : Science
Languages : en
Pages : 572
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Book Description
A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Author: Edward Chee Tak Yeung
Publisher: Springer
ISBN: 3319199447
Category : Science
Languages : en
Pages : 572
Get Book Here
Book Description
A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Author: Steven E. Ruzin
Publisher: Oxford University Press, USA
ISBN: 9780195089561
Category : Science
Languages : en
Pages : 322
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Book Description
Plant microtechnique has generated renewed interest in recent years, due in part to the need for molecular biologists to visualize a gene or gene product in the context of the whole plant. Plant Microtechnique and Microscopy offers uniquely in-depth coverage of this reinvigorated field. Thoroughly covering classical aspects of microscope slide preparation, it goes a step beyond all other available manuals by also documenting the theory and practice of modern applications. The text opens with single-page "Quick Start" protocols that provide students with fundamental instructions to complete eight of the most common microtechnique protocols used today. The following sections cover the theory and practice of microtechnique. The traditional paraffin method is demonstrated by explicit step-by-step protocols, and theoretical background is incorporated to give students the tools required to design their own experiments and to interpret existing results. In addition, modern applications such as methacrylate embedding and sectioning, microwave tissue processing, fluorescence histochemistry, and in situ hybridization are discussed in detail. The manual also contains a definitive chapter on microscopy and describes, in both text and diagrams, the optical principles of techniques such as phase contrast and DIC as well as confocal and deconvolution wide-field microscopy. Appendices on laboratory practice (chemical toxicities, common calculations, and buffer tables), an extensive appendix on optics and its application to the microscope, and an extensive bibliography of over 550 references are also included. Ideal for courses in plant biology, Plant Microtechnique and Microscopy also serves as an indispensable reference for all students of microscopy, histology, and histological technique. It is a valuable addition to every biological laboratory.
Author: D. A. Jahansen
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Duong Tan Nhut
Publisher: Springer Nature
ISBN: 9811664986
Category : Science
Languages : en
Pages : 400
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Book Description
This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems. This book will be of interest to teachers, researchers, scientists, capacity builders and policymakers. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, soil science, and environmental sciences.
Author: Edward Chee-Tak Yeung
Publisher: Springer Nature
ISBN: 1071640313
Category :
Languages : en
Pages : 410
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Book Description
Author: Jeremy Sanderson
Publisher: Garland Science
ISBN: 1000140946
Category : Science
Languages : en
Pages : 262
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Book Description
A completely new practical guide to both new and classical methods of slide-making which is easy-to-read and easy-to-understand. Biological Microtechnique contains a wealth of practical detail which will provide a firm grounding in preparative methods for light microscopy.
Author: Bénédicte Charrier
Publisher: CRC Press
ISBN: 1351132946
Category : Science
Languages : en
Pages : 515
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Book Description
This book presents a wide range of tested and proven protocols relevant to a number of fields within biotechnology used in laboratory experiments in everyday phycological (seaweed) research. A major focus will be on bioenergy related aspects of this emerging technology. These protocols will be written in a clear and concise manner using simple language permitting even nonspecialist to adequately understand the significance of this research. It will also contain all necesssary notes and guidelines for successful execution of these experiments.
Author: Richard Crang
Publisher: Springer
ISBN: 3319773151
Category : Science
Languages : en
Pages : 739
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Book Description
Intended as a text for upper-division undergraduates, graduate students and as a potential reference, this broad-scoped resource is extensive in its educational appeal by providing a new concept-based organization with end-of-chapter literature references, self-quizzes, and illustration interpretation. The concept-based, pedagogical approach, in contrast to the classic discipline-based approach, was specifically chosen to make the teaching and learning of plant anatomy more accessible for students. In addition, for instructors whose backgrounds may not primarily be plant anatomy, the features noted above are designed to provide sufficient reference material for organization and class presentation. This text is unique in the extensive use of over 1150 high-resolution color micrographs, color diagrams and scanning electron micrographs. Another feature is frequent side-boxes that highlight the relationship of plant anatomy to specialized investigations in plant molecular biology, classical investigations, functional activities, and research in forestry, environmental studies and genetics, as well as other fields. Each of the 19 richly-illustrated chapters has an abstract, a list of keywords, an introduction, a text body consisting of 10 to 20 concept-based sections, and a list of references and additional readings. At the end of each chapter, the instructor and student will find a section-by-section concept review, concept connections, concept assessment (10 multiple-choice questions), and concept applications. Answers to the assessment material are found in an appendix. An index and a glossary with over 700 defined terms complete the volume.
Author:
Publisher: Academic Press
ISBN: 0128215348
Category : Science
Languages : en
Pages : 458
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Book Description
Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis. - Provide in-depth procedures and application notes from selected experts who developed the methods - Each chapter will include figures and movies as appropriate to explain complex techniques - Chapters will include caveats of techniques and future prospects
Author: Nora A. Foroud
Publisher: Springer Nature
ISBN: 1071631594
Category : Science
Languages : en
Pages : 242
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Book Description
This detailed collection focuses on current methodologies for studying various crop diseases. The book explores phytopathogen detection, host-pathogen interactions at the earliest stages of colonization, RNA-seq analyses, as well as host responses downstream of, or coincidental to, gene expression and a newly established protocol for gene editing of Medicago sativa. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant-Pathogen Interactions serves as an ideal guide for researchers working in the vital area of plant diseases.
