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Author: Jeffrey B. Harborne
Publisher: Springer Science & Business Media
ISBN: 9400959214
Category : Science
Languages : en
Pages : 346
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Book Description
While there are many books available on methods of organic and biochemical analysis, the majority are either primarily concerned with the application of a particular technique (e.g. paper chromatography) or have been written for an audience of chemists or for biochemists work ing mainly with animaltissues. Thus, no simple guide to modern metho ds of plant analysis exists and the purpose of the present volume is to fill this gap. It is primarily intended for students in the plant sciences, who have a botanical or a general biological background. It should also be of value to students in biochemistry, pharmacognosy, food science and 'natural products' organic chemistry. Most books on chromatography, while admirably covering the needs of research workers, tend to overwhelm the student with long lists of solvent systems and spray reagents that can be applied to each class of organic constituent. The intention here is to simplify the situation by listing only a few specially recommended techniques that have wide currency in phytochemical laboratories. Sufficient details are provided to allow the student to use the techniques for themselves and most sections contain some introductory practical experiments which can be used in classwork.
Author: Jeffrey B. Harborne
Publisher: Springer Science & Business Media
ISBN: 9400959214
Category : Science
Languages : en
Pages : 346
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Book Description
While there are many books available on methods of organic and biochemical analysis, the majority are either primarily concerned with the application of a particular technique (e.g. paper chromatography) or have been written for an audience of chemists or for biochemists work ing mainly with animaltissues. Thus, no simple guide to modern metho ds of plant analysis exists and the purpose of the present volume is to fill this gap. It is primarily intended for students in the plant sciences, who have a botanical or a general biological background. It should also be of value to students in biochemistry, pharmacognosy, food science and 'natural products' organic chemistry. Most books on chromatography, while admirably covering the needs of research workers, tend to overwhelm the student with long lists of solvent systems and spray reagents that can be applied to each class of organic constituent. The intention here is to simplify the situation by listing only a few specially recommended techniques that have wide currency in phytochemical laboratories. Sufficient details are provided to allow the student to use the techniques for themselves and most sections contain some introductory practical experiments which can be used in classwork.
Author: A.J. Harborne
Publisher: Springer Science & Business Media
ISBN: 9780412572708
Category : Science
Languages : en
Pages : 504
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Book Description
This long awaited third edition of Phytochemical Methods is, as its predecessors, a key tool for undergraduates, research workers in plant biochemistry, plant taxonomists and any researchers in related areas where the analysis of organic plant components is key to their investigations. Phytochemistry is a rapidly expanding area with new techniques being developed and existing ones perfected and made easier to incorporate as standard methods in the laboratory. This latest edition includes descriptions of the most up-to-date methods such as HPLC and the increasingly sophisticated NMR and related spectral techniques. Other methods described are the use of NMR to locate substances within the plant cell and the chiral separation of essential oils. After an introductory chapter on methods of plant analysis, individual chapters describe methods of identifying the different type of plant molecules: phenolic compounds, terpenoids, organic acids, lipids and related compounds, nitrogen compounds, sugar and derivatives and macromolecules. Different methods are discussed and recommended, and guidance provided for the analysis of compounds of special physiological relevance such as endogenous growth regulators, substances of pharmacological interest and screening methods for the detection of substances for taxonomic purposes. It also includes an important bibliographic guide to specialized texts. This comprehensive book constitutes a unique and indispensable practical guide for any phytochemistry or related laboratory, and provides hands-on description of experimental techniques so that students and researchers can become familiar with these invaluable methods.
Author: Nikolaus H. Fischer
Publisher: Springer Science & Business Media
ISBN: 1468490605
Category : Science
Languages : en
Pages : 412
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Book Description
This volume contains reviews which are based on a symposium, given th at the 30 meeting of The Phytochemical Society of North America, held at Laval University in Quebec City, Canada on August 11-15, 1990. During the past two decades, there have been major new developments in methods which can be applied toward the isolation, separation and structure determination of complex natural products. Therefore, the topic of this symposium, "Modem Phytochemical Methods", is a very timely one. The organizers of the symposium recognized that it would not be possible to cover in detail all new advances in phytochemical methodology. It was therefore decided to emphasize general reviews on recent developments of major separation techniques such as high performance liquid chromatography as well as supercritical fluid chromato graphy. In addition, advances in commonly used structure determination methods, mainly NMR and MS, are reviewed. Other topics include methodo logies of micro-sampling for isolation and analysis of trichome constituents as well as recent breakthroughs on biosynthetic studies of monoterpenes using "enriched" basal cells of trichomes. The volume concludes with a review of quantitative structure-activity relationship (QSAR) studies of biologically active natural products. In Chapter I, K. Hostettmann and his colleagues give a general review of recent developments in the separation of natural products with major emphasis on preparative separations of biologically active plant constituents. The authors present a comparison of droplet countercurrent chromatography (OCCC) with the highly rapid and more versatile centrifugal partition chromatography (CPC).
Author: Prof. Dr. N. Raaman
Publisher: New India Publishing Agency
ISBN: 939017595X
Category : Technology & Engineering
Languages : en
Pages : 12
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Book Description
Phytochemicals are the individual chemicals from which the plants are made and plants are the key sources of raw materials for both pharmaceutical and aromatic industries. The improved methods for higher yield of active compounds will be the major incentive in these industries. To help those who involved in the isolation of compounds from plants, some of the essential phytochemical techniques are included in this book. It contains 10 chapters. A brief introduction is given in Chapter 1. Chapter 2 deals with the production processes for herbals and botanicals. Selection of plant and plant parts for phytochemical analysis are included in Chapter 3. Different methods of extraction are given in Chapter 4. Qualitative phytochemical screening is presented in Chapter 5. Various methods for separation of phytochemicals, which include paper and thin layer chromatography and column chromatography are given in Chapter 6. Qualitative and quantitative estimation of phytochemicals using gas chromatography, high performance liquid chromatography and high performance thin layer chromatography are described in Chapter 7. The various methods of identification including the physical characteristics and spectroscopy are included in Chapter 8. The ultraviolet spectroscopy, infrared spectroscopy, near infrared spectroscopy, mass spectroscopy, nuclear magnetic resonance spectroscopy and crystallography are included in this chapter. The categories of phytochemicals are given in Chapter 9. A case study of isolation and identification of compounds in the laboratory of the author of this book is included in Chapter 10. Isolation of alkaloids is given in Chapter 11. Extraction and isolation of phenolic compounds is described in Chapter 12. Isolation of anthocyanin compounds is included in Chapter 13. Extraction and analysis of essential oils are described in Chapter 14. The theoretical principles involved in the instruments, handling of samples and interpretation of spectra are given in detail. More than 160 figures (27 in colour) are included to illustrate the various techniques and the structures of compounds. Apart from the references, indexes of common and scientific names of plants and chemical names and subject index are included.
Author: Satyajit Dey Sarker
Publisher: Elsevier
ISBN: 0443161038
Category : Science
Languages : en
Pages : 532
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Book Description
Computational Phytochemistry, Second Edition, explores how recent advances in computational techniques and methods have been embraced by phytochemical researchers to enhance many of their operations, refocusing and expanding the possibilities of phytochemical studies. By applying computational aids and mathematical models to extraction, isolation, structure determination, and bioactivity testing, researchers can obtain highly detailed information about phytochemicals and optimize working approaches. This book aims to support and encourage researchers currently working with or looking to incorporate computational methods into their phytochemical work. Topics in this book include computational methods for predicting medicinal properties, optimizing extraction, isolating plant secondary metabolites, and building dereplicated phytochemical libraries. The roles of high-throughput screening, spectral data for structural prediction, plant metabolomics, and biosynthesis are all reviewed before the application of computational aids for assessing bioactivities and virtual screening is discussed. Illustrated with detailed figures and supported by practical examples, this book is an indispensable guide for all those involved with the identification, extraction, and application of active agents from natural products. This new edition captures remarkable advancements in mathematical modeling and computational methods that have been incorporated in phytochemical research, addressing, e.g., extraction, isolation, structure determination, and bioactivity testing of phytochemicals. - Includes step-by-step protocols for various computational and mathematical approaches applied to phytochemical research - Features clearly illustrated chapters contributed by highly reputable researchers - Covers all key areas in phytochemical research, including virtual screening and metabolomics
Author: N. Raaman
Publisher: Nipa
ISBN: 9788119103591
Category : Medical
Languages : en
Pages : 0
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Book Description
In the present book, to give more experimental data using the phytochemical techniques and medicinal plants, research papers, lead articles and review papers contributed by several researchers are included. This book contains 35 s from various experts covering medicinal plants and the experimental data using the methods of identification using physical characteristics and spectroscopy which includes UV, IR, NIR, Mass and NMR. In various s, antioxidant potentials of mushrooms, seaweeds and medicinal plants, isolation of compounds from plants and their biological activities, novel near infra red application in natural products, in silico studies of anti-inflammatory compounds, molecular docking of natural products, CNS depressant activity of medicinal plants, isolation of antifungal proteins from medicinal plants, plant tissue culture, in vitro cell line selection for enhanced production of secondary metabolites, isolation of compounds from cell suspension cultures, X-ray crystal structure analysis of compounds from medicinal plants, synthesis and bioactivity of polycyclic spiroheterocyclic compounds are given in details. This book will prove useful to the students, scientists, researchers, professionals in the field of Plant Science, Pharmaceutical, Chemical Engineering, Biotechnology, Medicinal and Aromatic Plants, Forestry and Horticulture.
Author: Zhimin Xu
Publisher: John Wiley & Sons
ISBN: 0813823919
Category : Technology & Engineering
Languages : en
Pages : 405
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Book Description
To quantify antioxidants in natural sources, the application of chromatography techniques with different detectors followed by skillful sample preparation is necessary. Analysis of Antioxidant-Rich Phytochemicals is the first book that specifically covers and summarizes the details of sample preparation procedures and methods developed to identify and quantify various types of natural antioxidants in foods. Focusing on the principle of quantification methods for natural antioxidants, the book reviews and summarizes current methods used in the determination of antioxidant-rich phytochemicals in different sources. Chapter by chapter, the distinguished team of authors describes the various methods used for analysis of the different antioxidant-rich phytochemicals – phenolic acids; carotenoids; anthocyanins; ellagitannins, flavonols and flavones; catechins and procyanidins; flavanones; stilbenes; phytosterols; and tocopherols and tocotrienols. Going beyond extensive reviews of the scientific literature, the expert contributors call on their accumulated experience in sample extraction and analysis to outline procedures, identify potential problems in dealing with different samples, and offer trouble-shooting tips for the analysis. Analysis of Antioxidant-Rich Phytochemicals covers the important food applications and health-promoting functions of the major antioxidant phytochemicals, presents general analysis principles and procedures, and systematically reviews and summarizes the various analytical methods necessary for each type of natural antioxidant in different food sources.
Author: Peter Houghton
Publisher: Springer Science & Business Media
ISBN: 1461558093
Category : Science
Languages : en
Pages : 205
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Book Description
This laboratory manual will be welcomed by all research scientists involved in the extraction, fractionation and isolation of compounds from natural materials, especially those working with plants. The book is clear and concise, and features practical exercises to illustrate the techniques described in every chapter. It will provide an invaluable research reference tool for those scientists investigating the potential benefits of ethnomedicine and the properties of chemicals isolated from natural flora.
Author: Thangaraj Parimelazhagan
Publisher: Springer
ISBN: 3319268112
Category : Medical
Languages : en
Pages : 182
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Book Description
This volume provides information on how to select and screen plants for their medicinal properties. It describes phytopharmacological techniques for extracting and qualitatively and quantitatively analyzing a plant’s phytochemicals. After a detailed in vitro investigation including nutritional and anti-nutritional analyses, medicinal properties were tested with various in vivo models for anti-inflammatory, analgesic, anti-pyretic, anticancer and anti-diabetic properties, as well as wound healing, neurodegenerative diseases, etc. Compound identification and purification techniques include, among others, TLC and column chromatography, as well as molecular docking with specific proteins.
Author: Monika Waksmundzka-Hajnos
Publisher: CRC Press
ISBN: 1420092618
Category : Health & Fitness
Languages : en
Pages : 998
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Book Description
The powerful, efficient technique of high performance liquid chromatography (HPLC) is essential to the standardization of plant-based drugs, identification of plant material, and creation of new herbal medicines. Filling the void in this critical area, High Performance Liquid Chromatography in Phytochemical Analysis is the first book to give a comp
