Plant Cell, Tissue and Organ Culture

Plant Cell, Tissue and Organ Culture PDF Author: Oluf Gamborg
Publisher: Springer Science & Business Media
ISBN: 3642790488
Category : Science
Languages : en
Pages : 367

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Book Description
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Plant Cell, Tissue and Organ Culture

Plant Cell, Tissue and Organ Culture PDF Author: Oluf Gamborg
Publisher: Springer Science & Business Media
ISBN: 3642790488
Category : Science
Languages : en
Pages : 367

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Book Description
This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture

Applied and Fundamental Aspects of Plant Cell, Tissue, and Organ Culture PDF Author: Jakob Reinert
Publisher: Springer Science & Business Media
ISBN: 3662022796
Category : Science
Languages : en
Pages : 810

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Book Description
Progress in the field of plant cell and tissue culture has made this area of research one of the most dynamic and promising not only in plant physiology, cell biology and genetics but also in agriculture, forestry, horticulture and industry. Studies with plant cell cultures clearly have bearing upon a variety of problems as yet unsolved in basic and applied research. This was the compelling reason for assembling such a comprehensive source of information to stimulate students, teachers, and research workers. This book comprises 34 articles on regeneration of plants, vegetative propagation and cloning; haploids; cytology, cytogenetics and plant breeding; protoplasts, somatic hybridization and genetic engineering; plant pathology; secondary products and a chapter on isoenzymes, radiobiology, and cryobiology of plant cells. Particular attention has been paid to modern , fast-growing and fascinating disciplines - e.g. the induction of haploids, somatic hybridization and genetic manipulation by protoplast culture, which possess an enormous potential for plant improvement.

The Organ in Western Culture, 750-1250

The Organ in Western Culture, 750-1250 PDF Author: Peter Williams
Publisher: Cambridge University Press
ISBN: 9780521617079
Category : Music
Languages : en
Pages : 422

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Book Description
How did the organ become a church instrument? In this fascinating investigation Peter Williams speculates on this question and suggests some likely answers. Central to the story he uncovers is the liveliness of European monasticism around 1000 and the ability and imagination of the Benedictine reformers.

Organ Culture

Organ Culture PDF Author: J Thomas
Publisher: Elsevier
ISBN: 0323147119
Category : Medical
Languages : en
Pages : 527

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Book Description
Organ Culture provides information pertinent to the fundamental aspects of the study of autodifferentiation. This book discusses the physiological differentiation, the action of substances that inhibit or stimulate growth, and the interactions of associated organs. Organized into 10 chapters, this book begins with an overview of the differentiation of embryonic organs in natural media. This text then presents the study of differentiation in synthetic solid or liquid media and explains the biochemical and physiological requirements of the tissues. Other chapters consider the problems of sexual differentiation and intersexuality in vitro by providing a comparative study of avian and mammalian embryos. This book analyzes as well the interactions between hormones, inhibitor substances, and receptor organs in vitro. The final chapter deals with the study of organ chimeras in vitro and the related study of the organ culture of malignant tumors. This book is a valuable resource for embryologists and research workers.

Tissue Culture Techniques for Horticultural Crops

Tissue Culture Techniques for Horticultural Crops PDF Author: Kenneth C. Torres
Publisher: Springer Science & Business Media
ISBN: 1461597560
Category : Technology & Engineering
Languages : en
Pages : 282

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Book Description
This book was written for those individuals who are concerned about the techniques and practices of plant cell cultures for horticultural crops. It was designed to serve as a text and reference for students and professionals in ornamental horticulture, fruit and vegetable crop pro duction, botany, forestry, and other areas of plant science. Research during the last twenty-five years in the area of plant tissue culture has led to many developments and changes in this field. Al though the techniques involved in the manipulation of plant tissue culture are now relatively straightforward, the presentation of these techniques in a short volume for the beginner in the field is generally unavailable. In addition to describing the techniques for establishment and manipulation of specific species, several chapters in this book also provide a brief, general review of important cultural parameters. Spe cific protocols and laboratory procedures may also be found in the appendix. I hope that this presentation of information will be helpful to those individuals wanting to apply plant tissue culture techniques for horticultural crops.

In Vitro Culture of Mycorrhizas

In Vitro Culture of Mycorrhizas PDF Author: Stéphane Declerck
Publisher: Springer Science & Business Media
ISBN: 9783540240273
Category : Nature
Languages : en
Pages : 418

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Book Description
This is the first book describing in vitro cultivation of root organs. The text describes various biological aspects such as the physiology, biochemistry, biodiversity, and life cycles of fungi, as well as the effects of symbiosis on plant growth and development, including large-scale fungus production for biotechnological use. Detailed protocols allow the immediate application of the method to culture mycorrhizal fungi in vitro.

Practical Approach to Mammalian Cell and Organ Culture

Practical Approach to Mammalian Cell and Organ Culture PDF Author: Tapan Kumar Mukherjee
Publisher: Springer Nature
ISBN: 9811917310
Category : Medical
Languages : en
Pages : 1286

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Book Description
This Major Reference Work offers a detailed overview of culturing primary, secondary cell lines, tissues, and organs. It first introduces various types of mammalian cell cultures, infrastructure requirements for a mammalian cell-culture laboratory. The subsequent chapters present the detailed protocols for the isolation of mammalian hematologic organs and cells. It also discusses various cell-based assays for monitoring cell viability, cell proliferation, cytotoxicity, cell senescence, and cell death assays. In addition, the book addresses the various problems encountered while culturing animal cells, their possible causes, and suggested solutions, presenting detailed protocols for isolation and primary culturing of various mammalian cells and hematoimmunologic organs in two dimensions. Lastly, it reviews the various applications of animal-cell culture, stem-cell culture, and tissue and organ culture. As such, this reference book is highly relevant for students and professionals new to cell-culture work as well as to those wishing to expand their skills from cell-line cultures to primary cultures and from conventional 2D cultures to 3D cultures.

In Vitro Culture of Higher Plants

In Vitro Culture of Higher Plants PDF Author: R.L.M Pierik
Publisher: Springer Science & Business Media
ISBN: 9401157502
Category : Science
Languages : en
Pages : 346

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Book Description
In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.

Molecular Biology of the Cell

Molecular Biology of the Cell PDF Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0

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Book Description


INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE

INTRODUCTION TO PLANT CELL TISSUE AND ORGAN CULTURE PDF Author: SUNIL D. PUROHIT
Publisher: PHI Learning Pvt. Ltd.
ISBN: 8120346777
Category : Science
Languages : en
Pages : 334

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Book Description
Designed primarily as a text for undergraduate and postgraduate students of Botany and Plant Biotechnology, the book discusses the theoretical aspects and modern applications of plant cell, tissue and organ culture. Written with the aim of providing up-to-date information on the subject, and focused on the concept of commercialization of plant cell culture, the contents have been presented with clarity. The book not only discusses the theoretical aspects of plant tissue culture but also emphasizes the art of its practice. It also provides a systematic explanation of asepsis and methods of sterilization, plant tissue culture techniques, culture of reproductive structures, plant tissue culture in germplasm conservation, its applications in the industry and plant pathology and operation and management of greenhouse hardening unit. In addition, it discusses in vitro propagation of plants (micropropagation) with a series of case studies pertaining to tree species and horticultural crops. Besides students, the book will also prove to be useful for researchers, scholars and teachers.