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Author: Russell F. Doolittle
Publisher: University Science Books
ISBN: 9780935702545
Category : Science
Languages : en
Pages : 118
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Book Description
In these days of facile cloning and rapid DNA sequencing, it is not uncommon for investigators to find themselves with a DNA sequence that may or may not code for a known gene product. The sequence is 'open' when read in an appropriate frame, which is to say that there is a long run of amino acid codons before the appearance of a terminator codon. How can we find out if this 'unidentified reading frame' (URF) really codes for a genuine protein, and how can we identify it if it exists? There are two general strategies, both of which can also be applied to the characterization of any 'open reading frame' (ORF), whether or not it has been 'identified'. The first and simplest approach involves computer searching and analysis; the second employs antibodies raised against synthetic peptides patterned on the sequence of the expected gene product. Both methods have been used with great success by many investigators. Each has, nonetheless, its pitfalls and frustrations. This primer is meant to guide the researcher past those obstacles as much as possible. Graduate students and researchers interested in amino acid sequencing; molecular biologists, biochemists, chemists, and biotechnologists.
Author: Russell F. Doolittle
Publisher: University Science Books
ISBN: 9780935702545
Category : Science
Languages : en
Pages : 118
Get Book
Book Description
In these days of facile cloning and rapid DNA sequencing, it is not uncommon for investigators to find themselves with a DNA sequence that may or may not code for a known gene product. The sequence is 'open' when read in an appropriate frame, which is to say that there is a long run of amino acid codons before the appearance of a terminator codon. How can we find out if this 'unidentified reading frame' (URF) really codes for a genuine protein, and how can we identify it if it exists? There are two general strategies, both of which can also be applied to the characterization of any 'open reading frame' (ORF), whether or not it has been 'identified'. The first and simplest approach involves computer searching and analysis; the second employs antibodies raised against synthetic peptides patterned on the sequence of the expected gene product. Both methods have been used with great success by many investigators. Each has, nonetheless, its pitfalls and frustrations. This primer is meant to guide the researcher past those obstacles as much as possible. Graduate students and researchers interested in amino acid sequencing; molecular biologists, biochemists, chemists, and biotechnologists.
Author: Abraham Marcus
Publisher: Elsevier
ISBN: 032316336X
Category : Science
Languages : en
Pages : 725
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Book Description
The Biochemistry of Plants, Volume 15: Molecular Biology presents information pertinent to gene expression, cytoskeletal proteins, and hydroxyproline-rich glycoprotein. This book discusses the specific gene systems and examines the regulatory regions within the genes. Organized into 17 chapters, this volume starts with an overview of the important mechanism for regulating gene expression, which is significant in the selective turnover of gene products. This book then proceeds with a discussion of the concept of protein degradation and the extracellular carriers of genetic information. Other chapters review the viral and plasmid systems, which are relevant to plants. This text discusses as well the phenotypic changes in plants, including plant genetic tumor and habituated plant tissues that exhibit hormone autotrophic growth. The final chapter examines the importance of genetic manipulation at the cellular level via protoplast fusion, cell selection, and transformation. Biologists, biochemists, enzymologists, biophysicists, and plant scientists will find this book extremely useful.
Author: Michael Fortun
Publisher: Springer Science & Business Media
ISBN: 9401147183
Category : Science
Languages : en
Pages : 236
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Book Description
That concern about human genetics is at the top of many lists of issues requiring intense discussion from scientific, political, social, and ethical points of view is today no surprise. It was in the spirit of attempting to establish the basis for intelligent discussion of the issues involved that a group of us gathered at a meeting of the International Society for the History, Philosophy, and Social Studies of Biology in the Summer of 1995 at Brandeis University and began an exploration of these questions in earlier versions of the papers presented here. Our aim was to cross disciplines and jump national boundaries, to be catholic in the methods and approaches taken, and to bring before readers interested in the emerging issues of human genetics well-reasoned, informative, and provocative papers. The initial conference and elements of the editorial work which have followed were generously supported by the Stifterverband fUr die Deutsche Wissenschaft. We thank Professor Peter Weingart of Bielefeld University for his assistance in gaining this support. As Editors, we thank the anonymous readers who commented upon and critiqued many of the papers and in tum made each paper a more valuable contribution. We also thank the authors for their understanding and patience. Michael Fortnn Everett Mendelsohn Cambridge, MA September 1998 vii INTRODUCTION In 1986, the annual symposium at the venerable Cold Spring Harbor laboratories was devoted to the "Molecular Biology of Homo sapiens.
Author: Hooman H. Rashidi
Publisher: CRC Press
ISBN: 9780849323751
Category : Science
Languages : en
Pages : 210
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Book Description
Scientists in the throes of research use an extensive data bank to access structural information on proteins and nucleic acids. Meanwhile, geneticists use a highly specialized form of software to analyze the hybridization pattern of DNA chips. The past decade has been tremendously successful for biotechnology and pharmaceutical industries. This success has been a result of astounding technical advancements in genome sequencing (genomics), protein identification (proteomics), and data analysis-collectively called bioinformatics. The exponential increase in computer processing and disk storage has served as a catalyst and been instrumental in the development of bioinformatics. Bioinformatics Basics: Applications in Biological Science and Medicine describes the origin of this field and the organization of public domain databases with an introductory tutorial for the services described. This book explains these services in a relatively simple fashion. Nevertheless, a biological background is necessary to understand and properly utilize the various software components and services described in this book. A basic biological background is also necessary for understanding the biological and medical significance of the collected data. Bioinformatics Basics is a fast growing field, and it will take some years for a stabilization to occur. Until then, hundreds of Internet sites allow us to search, compare, and manipulate this data in its relatively raw format. Bioinformatics Basics: Applications in Biological Science and Medicine concentrates on three major database clusters and relevant software tools that are maintained in the United States, Europe, and Japan, offering free access and analysis through the Internet.
Author: H.O. Villar
Publisher: Elsevier
ISBN: 9780080526119
Category : Science
Languages : en
Pages : 267
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Book Description
The second volume in a series which aims to focus on advances in computational biology. This volume discusses such topics as: statistical analysis of protein sequences; progress in large-scale sequence analysis; and the architecture of loops in proteins.
Author:
Publisher: Academic Press
ISBN: 9780080580005
Category : Science
Languages : en
Pages : 473
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Book Description
Advances in Microbial Physiology
Author: Mark Walker
Publisher: John Wiley & Sons
ISBN: 1444313606
Category : Science
Languages : en
Pages : 336
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Book Description
Route Maps in Gene Technology is an exciting new introductory textbook for first-year undergraduates in molecular biology and molecular genetics. The subject is broken down into 140 to 150 key concepts or topics, each of which is dealt with in one doublepaged spread. These range from basic introductory principles to applied topics at the cutting edge of research. A control strip along the top of the page shows the student which pages need to have been read beforehand and which topics may be followed afterward. In addition, at the front of the book are a selection of 'routes,' which the student or teacher may choose in order to study a particular topic. Because courses have become more 'modular' and many students arrive at college with little or no biology background, this approach enables teachers and students to structure a course of study to best suit their disparate exposure to biology. An exciting new concept in textbook design, allowing unparalleled flexibility on the part of the student and the teacher Covers the full range of modern molecular biology, from basic principles to the latest applications Attractive, clear and simple presentation with copious two-colour illustrations
Author: Donald Voet
Publisher: John Wiley & Sons
ISBN: 0470917458
Category : Science
Languages : en
Pages : 1520
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Book Description
The "Gold Standard" in Biochemistry text books, Biochemistry 4e, is a modern classic that has been thoroughly revised. Don and Judy Voet explain biochemical concepts while offering a unified presentation of life and its variation through evolution. Incorporates both classical and current research to illustrate the historical source of much of our biochemical knowledge.
Author: Tony Stankus
Publisher: Routledge
ISBN: 1000759083
Category : Language Arts & Disciplines
Languages : en
Pages : 175
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Book Description
In this book, first published in 1992, science librarians analyse the life and times of small liberal arts college science libraries and the workday life of librarians serving scientists from a main campus library. They describe their efforts to defend expensive science collections in the face of tight budgets, to singlehandedly monitor and select literature in all areas from astronomy through zoology, and to compete with the humanities and social studies for library shelf space.
Author: Gary C. Howard
Publisher: CRC Press
ISBN: 1420036521
Category : Medical
Languages : en
Pages : 272
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Book Description
In recent years, interest in proteins has surged. This resurgence has been driven by the expansion of the post-genomic era when structural genomics and proteomics require new techniques in protein chemistry and new applications of older techniques. Protein chemistry methods are used by nearly every discipline of biomedical research. Many techniques
