Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine PDF Download
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Author: Shusheng Zhang
Publisher: Springer
ISBN: 9811370443
Category : Science
Languages : en
Pages : 337
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Book Description
This book describes the rational design, development and application of nucleic acid amplification strategies for biosensing, bioimaging and biomedicine. It consists of fifteen chapters demonstrating the use of these strategies in various areas, including fluorescence techniques, Chemiluminescence biosensors, electrochemiluminescence biosensors, colorimetric assays, surface plasmon resonance technologies, electrochemical DNA sensors, photoelectrochemical biosensor, nanopore sensors, quartz crystal microbalance, fluorescence imaging, surface-enhanced Raman spectroscopy, in vitro and in vivo metal ions detection, theranostics and microdroplet chips. Offering a collection of reviews illustrating the latest advances in biochemical analysis and therapeutics, the book shares valuable insights into current challenges and future prospects, making it a valuable resource for a wide readership in the various fields of biosensing, bioimaging and biomedicine.
Author: Shusheng Zhang
Publisher: Springer
ISBN: 9811370443
Category : Science
Languages : en
Pages : 337
Get Book Here
Book Description
This book describes the rational design, development and application of nucleic acid amplification strategies for biosensing, bioimaging and biomedicine. It consists of fifteen chapters demonstrating the use of these strategies in various areas, including fluorescence techniques, Chemiluminescence biosensors, electrochemiluminescence biosensors, colorimetric assays, surface plasmon resonance technologies, electrochemical DNA sensors, photoelectrochemical biosensor, nanopore sensors, quartz crystal microbalance, fluorescence imaging, surface-enhanced Raman spectroscopy, in vitro and in vivo metal ions detection, theranostics and microdroplet chips. Offering a collection of reviews illustrating the latest advances in biochemical analysis and therapeutics, the book shares valuable insights into current challenges and future prospects, making it a valuable resource for a wide readership in the various fields of biosensing, bioimaging and biomedicine.
Author: Shusheng Zhang
Publisher:
ISBN: 9789811370458
Category : Nucleic acids
Languages : en
Pages :
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Book Description
This book describes the rational design, development and application of nucleic acid amplification strategies for biosensing, bioimaging and biomedicine. It consists of fifteen chapters demonstrating the use of these strategies in various areas, including fluorescence techniques, Chemiluminescence biosensors, electrochemiluminescence biosensors, colorimetric assays, surface plasmon resonance technologies, electrochemical DNA sensors, photoelectrochemical biosensor, nanopore sensors, quartz crystal microbalance, fluorescence imaging, surface-enhanced Raman spectroscopy, in vitro and in vivo metal ions detection, theranostics and microdroplet chips. Offering a collection of reviews illustrating the latest advances in biochemical analysis and therapeutics, the book shares valuable insights into current challenges and future prospects, making it a valuable resource for a wide readership in the various fields of biosensing, bioimaging and biomedicine.
Author: Laís Canniatti Brazaca
Publisher: Elsevier
ISBN: 0443153817
Category : Science
Languages : en
Pages : 409
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Book Description
Biosensors in Precision Medicine: From Fundamentals to Future Trends covers important topics regarding biomarkers, including biomarker discovery, validation, application in precision medicine, the principles of biosensors, their use in precision medicine, important analytical parameters, recent advances in bioreceptors and transduction, and more. Finally, the hottest trends of biosensors in precision medicine, including lab-on-a-chip and wearable devices, advances towards telemedicine, machine learning, artificial intelligence and the commerciality of these devices are discussed. Provides a timely review of the state-of-the-art developments in biosensors and their applications in precision medicine Presents an evaluation of case studies, along with real-life examples Addresses recent trends on biosensors for precision medicine
Author: Helen H. Lee
Publisher: Springer Science & Business Media
ISBN: 9780817639211
Category : Medical
Languages : en
Pages : 300
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Book Description
Providing current information and guidance on the uses of various nucleic acid amplification technologies for clinical laboratory diagnosis, this book goes beyond the Polymerase Chain Reaction to explore a broader range of important alternative DNA/RNA amplification methods including the Ligase Chain Reaction, Q[beta] Replicase Assays and TMA. There are many examples of specific applications of these technologies, discussions of yet unresolved issues and demonstrations of the relevance of these technologies to medical research and disease diagnostics. Individual chapters cover uses of these methods in clinical situations such as detection of food pathogens, viral infections, STDs, Mycobacteria drug resistance mutations, and heritable diseases. Automation, diagnostic test evaluation, and the synthesis of artificial DNA are also discussed. This book is designed for all biomedical scientists interested in the application of molecular biology to clinical diagnosis.
Author: Dmitry M. Kolpashchikov
Publisher: Humana Press
ISBN: 9781627035361
Category : Medical
Languages : en
Pages : 320
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Book Description
In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
Author: Gary A. Toranzos
Publisher: CRC Press
ISBN: 9781566764087
Category : Science
Languages : en
Pages : 240
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Book Description
From the Preface Antibody techniques have allowed us to study microorganisms in situ. However, until recently all methodology lacked the sensitivity necessary for environmental work where microorganisms are in most cases present at very low concentrations or where microbial ecosystems contain a myriad of different organisms. Gene probes have been used successfully for a variety of samples, but this method was still not sensitive enough. The next logical step was the application of the recently developed DNA amplification technique known as the polymerase chain reaction, or PCR. Since then, many laboratories around the world have adopted PCR for environmental work. Samples obtained from soils, water and air are enormously complex because they are unknown mixtures of DNA and other compounds. Thus, procedures for target DNA amplification from the environment require special attention. The PCR has allowed us to go beyond the need for culturing prior to analysis of microbial communities. It has been shown that even microorganisms that can be routinely grown in the laboratory undergo some physiological changes when exposed to the environment. One of these changes (first observed by R. Colwell and colleagues) is known as the viable-but-non-culturable state, and seems to be a common occurrence. Thus, the use of culture techniques paint only part of the picture in terms of microbial behavior under environmental conditions. The ability to amplify nucleic acids by the PCR has brought about a myriad of very ingenious modifications to the technique that can then be used to study complex ecosystems. The manner in which the PCR can be modified is only limited by the need and/or the imagination of the researcher. The first manual dedicated specifically to the analysis (by PCR) of environmental samples, Environmental Applications of Nucleic Acid Amplification Techniques presents state of the art methodology for the detection of microorganisms in soil, water, air samples, as well as the amplification of nucleic acids from fossil samples. The manual gives step-by-step procedures for the analysis of these samples. Although several publications have addressed the use of Polymerase Chain Reaction technique, very few of them have been directed toward the application of this technique to environmental samples. This book fills this gap in the literature.
Author: Ralph Rapley
Publisher: Springer Science & Business Media
ISBN: 1592590381
Category : Science
Languages : en
Pages : 997
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Book Description
A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."
Author: Sushma Dave
Publisher: CRC Press
ISBN: 1040111084
Category : Technology & Engineering
Languages : en
Pages : 323
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Book Description
Point of care (POC) diagnostic devices are predominantly used for the diagnosis and monitoring of diseases. To make these technologies scalable for manufacturing, user-friendly, inexpensive, sensitive, and rapid, a combination of such devices with nanomaterials is required. This book deals with new emerging fields such as POC technologies and advanced nanotheranostics using nanomaterials and their technologies and applications in diagnosis. In this book, current advances for the application of nanomaterials such as carbon nanotubes, graphene, and magnetic nanoparticles in POC devices and future directions are reviewed. This book: Presents a comprehensive account of needs and challenges of POC diagnostics Describes the fundamentals of rationale of nanomaterials as remarkable building blocks for biosensing Discusses development of critical diagnosis in POC systems Deals with the advantages of nanomaterial-based sensing strategies Illustrates the challenges and breakthroughs of technologies for cost-efficient biosensing platform The book is aimed at researchers and professionals in nanotechnology and biomedical engineering.
Author: Yiyang Dong
Publisher: Elsevier
ISBN: 0323985149
Category : Science
Languages : en
Pages : 352
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Book Description
Aptamers for Food Applications: Safety, Authenticity, and Integrity outlines the main applications of aptamers to ensure food safety, quality and compliance that are crucial for food science, public health and human sustainability. Aptamers or aptamer-based analytical devices are comprehensively summarized to tackle various food contamination or food adulteration problems. Topics covered in this book include aptamers for foodborne microbial pathogens, pesticide or veterinary drug residues, heavy metals, biotoxins, abused food additives, illegal food adulterants, POPs, GMOs, food allergens and food contact material migrants. It is therefore of interest for not only analytical investigators and food safety inspectors, but also regulatory practitioners and risk assessors in various food science and research fields. Presents the versatilities of aptamers for food safety, quality and compliance Provides utilities of aptamers for the analysis of almost all risk factors in foodstuffs Includes food quality control and regulatory measures such as HACCP, MRLs, proficiency test and uncertainty assessment, along with case illustrations
Author: Ashley Meagan Newbigging
Publisher:
ISBN:
Category : Biochemical markers
Languages : en
Pages : 0
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Book Description
The early detection of disease is beneficial for improved patient prognoses. One major challenge in early disease detection is the small, undetectable amounts of biomarkers. Detection strategies that confer amplification of the biomarker itself or of the detection signal are therefore desirable. Furthermore, emerging strategies conferring amplification at isothermal temperatures offer improvements in technical procedures by circumventing the requirement for multiple different reaction temperatures as is required in polymerase chain reaction. Despite the advances in isothermal amplification strategies using nucleic acids, there are still some drawbacks such as the technical difficulty of protocols and number of reagents required. The primary objective of my research was to develop novel techniques to improve and simplify the detection of nucleic acid and protein targets. In order to address this objective, I developed three new techniques for the isothermal and amplified detection of nucleic acid and protein targets with improved features. To improve isothermal and exponential amplified detection, I developed a new technique called Beacon-mediated Exponential Amplification Reaction (BEAR) to detect nucleic acid targets. BEAR only required a single enzyme and a single primer. I applied BEAR to detect Myoclonus Epilepsy with Ragged Red Fibres (MERRF). I achieved a limit of detection of 10 fM in 80 min and a recovery of ~91% in cell lysate for the MERRF sequence using BEAR. To improve isothermal and amplified detection without using enzymes, I developed a new turn-on fluorescence technique inspired by hybridization chain reaction (HCR) enabling the generation of turn-on fluorescence signals from label-free hairpins. This HCR technique uses four hairpins which overcomes the background that could arise when using two label-free hairpins. Using this new technique, I achieved a limit of detection of 660 pM of a nucleic acid target in solution when using 50 nM of hairpins in 30 min at room temperature. To improve the protocols of localized protein imaging, I adapted the concept of bindinginduced DNA assembly (BINDA) to the developed HCR technique. I used BINDA to convert protein binding into the generation of a DNA strand. The formation of the DNA strand initiated the HCR that produced fluorescence signals. I applied this technique to detect a HER2+ breast cancer cell line where membrane fluorescence indicating the HER2 status of the cells was achieved in as soon as 5 min with strong fluorescence signals at about 45 min to 60 min. This technique did not require any enzymes or washing steps and was performed at room temperature. These developed techniques feature isothermal reaction temperatures, low reaction volumes, and technically simple protocols because they are all in mix-and-read formats. These features allow potential applications of my techniques for improved clinical laboratory testing, point-of-care assays, and testing in resource-limited settings. Furthermore, the modularity of these developed DNA designs allows for the adaptation to other targets as well.
