Non-Radioactive Labelling PDF Download
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Author: A. J. Garman
Publisher: Elsevier
ISBN: 0080551092
Category : Science
Languages : en
Pages : 155
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Book Description
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules.Aimed at researchers without specific expertise in chemistry, the book includes: - A review of the main signal systems and labels available, indicating their strengths and weaknesses - Discussion of the most useful strategies for labelling the various biomolecules - 32 protocols covering common labelling needs - Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications - Sources of information including references, data and suppliers
Author: A. J. Garman
Publisher: Elsevier
ISBN: 0080551092
Category : Science
Languages : en
Pages : 155
Get Book Here
Book Description
Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules.Aimed at researchers without specific expertise in chemistry, the book includes: - A review of the main signal systems and labels available, indicating their strengths and weaknesses - Discussion of the most useful strategies for labelling the various biomolecules - 32 protocols covering common labelling needs - Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications - Sources of information including references, data and suppliers
Author: James C.E. Underwood
Publisher: Springer Science & Business Media
ISBN: 3642746683
Category : Medical
Languages : en
Pages : 491
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Book Description
New techniques make it possible for investigators to examine and sometimes quantify various aspects of nuclear morphology and function; now they can derive clinically and biologically useful information about the nucleus. This book draws together a series of techniques which have been successfully applied to the study of the nucleus of tumour cells. These are of fundamental importance to researchers in tumour histopathology and medical oncology. Detailed reviews are given of various aspects of the morphology, ploidy and karyotypic status and function of the nuclei in the cells of tumours and preneoplastic lesions. Topics covered include nuclear morphology in tumour diagnosis, the ultrastructure of the nucleus, karyotypic analyses of solid tumours, flow cytometric assessment of nuclear ploidy and other parameters, histomorphometry of the nucleus, and in situ hybridisation.
Author: Stefan Surzycki
Publisher: Springer Science & Business Media
ISBN: 3642569684
Category : Science
Languages : en
Pages : 442
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Book Description
This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.
Author: Christoph Kessler
Publisher: Springer
ISBN: 3662001446
Category : Science
Languages : en
Pages : 449
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Book Description
All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.
Author: S. Avrameas
Publisher: Elsevier Science & Technology
ISBN:
Category : Medical
Languages : nl
Pages : 428
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Book Description
Aandacht voor de enzymatische-immunocytochemie met de nadruk op intracellulaire penetratie van gelabelde antilichamen en de toepassing van "double staining"-methodes of monoclonale antilichamen in normale en in diverse experimentele of pathologische omstandigheden; kwantitatieve aspecten van immuno-enzymatische technieken, zoals nieuwe technische vindingen en toepassingen op verschillende gebieden, bijvoorbeeld biologie, humane en veterinaire geneeskunde, fytopathologie en experimentele ziekten
Author: U.S. Department of Transportation
Publisher: Simon and Schuster
ISBN: 1626363765
Category : House & Home
Languages : en
Pages : 352
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Book Description
Does the identification number 60 indicate a toxic substance or a flammable solid, in the molten state at an elevated temperature? Does the identification number 1035 indicate ethane or butane? What is the difference between natural gas transmission pipelines and natural gas distribution pipelines? If you came upon an overturned truck on the highway that was leaking, would you be able to identify if it was hazardous and know what steps to take? Questions like these and more are answered in the Emergency Response Guidebook. Learn how to identify symbols for and vehicles carrying toxic, flammable, explosive, radioactive, or otherwise harmful substances and how to respond once an incident involving those substances has been identified. Always be prepared in situations that are unfamiliar and dangerous and know how to rectify them. Keeping this guide around at all times will ensure that, if you were to come upon a transportation situation involving hazardous substances or dangerous goods, you will be able to help keep others and yourself out of danger. With color-coded pages for quick and easy reference, this is the official manual used by first responders in the United States and Canada for transportation incidents involving dangerous goods or hazardous materials.
Author: Keith Wilson
Publisher: Cambridge University Press
ISBN: 9780521658737
Category : Medical
Languages : en
Pages : 812
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Book Description
New edition of biochemistry textbook which introduces principles and techniques used in undergraduate practical classes.
Author: Melody S. Clark
Publisher: Springer Science & Business Media
ISBN: 3642878733
Category : Science
Languages : en
Pages : 551
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Book Description
Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author: Robert E. Farrell Jr.
Publisher: Elsevier
ISBN: 0080454763
Category : Science
Languages : en
Pages : 794
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Book Description
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects
Author: John M. Walker
Publisher: Springer Science & Business Media
ISBN: 1603273751
Category : Science
Languages : en
Pages : 1105
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Book Description
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
