Author: Nicola McGillicuddy
Publisher:
ISBN:
Category :
Languages : en
Pages : 200
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This study was dedicated to the development and characterisation of novel stationary phases for the detection and separation of metal cations in high performance chelation ion chromatography (HPCIC). Standard ion exchange chromatography is often unsuitable for the analysis of metals in complex samples as the ion exchange sites can become swamped by the presence of alkali metal salts. Commonly used spectroscopic techniques can be costly and time consuming due to the requirement of sample dilution and/or preconcentration, which can affect sensitivity and limits of detection. Furthermore, many of these methods also require a large sample volume and are unable to tolerate even minute quantities of salts in complex matrices. HPCIC has proven to be a promising alternative to spectroscopic detection techniques and standard ion exchange chromatography, mainly due to the ease in which the selectivity for metal cations can be manipulated. Therefore, in the work presented herein, a number of stationary phases of different morphologies were fabricated and/or characterised for the determination of metal cations in HPCIC. Firstly, polymeric and silica based columns functionalised with IDA were compared for their chromatographic behaviour for selected metal cations. A commercially available silica monolith column was covalently modified with Nhydroxyethyliminodiacetic (HEIDA) groups and successfully characterised for alkaline earth and selected transition and heavy metal cations, achieving peak efficiencies of 54,000, 60,000 and 64,000 N/m, for Zn2+, Mn2+ and Cd2+. The monolithic HEIDA-silica column and a N-(2-hydroxyethyl)-N- (2[phosphonomethyl)amino]acetic (HEPMA) modified silica monolith with alternative selectivity were applied to the development of a new HPCIC method for the quantitative determination of Mn2+, Cd2+ and Zn2+ in mussel digest samples. Finally, a chelating HEIDA modified core-shell stationary phase was fabricated and characterised for a number of metal cations including rare earth elements. The stationary phase was fully characterised and showed peak efficiencies (>200,000 N/m) far exceeding those previously observed on any IDA silica stationary phase. The work carried out proved HPCIC to be a convenient and efficient analytical method for the determination and separation of metal cations in complex samples, and a possible alternative to the aforementioned spectroscopic techniques.
Author: Owen John Challenger
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Stuart Drew Chambers
Publisher:
ISBN:
Category : Ion exchange chromatography
Languages : en
Pages : 390
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Author: Lianjia Ma
Publisher:
ISBN:
Category :
Languages : en
Pages : 484
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Author: Brian Charles Trammell
Publisher:
ISBN:
Category :
Languages : en
Pages : 370
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Author: Mohammad Talebi
Publisher:
ISBN:
Category :
Languages : en
Pages : 330
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Author: Antonio Gianguzza
Publisher: Springer Science & Business Media
ISBN: 9783540666189
Category : Nature
Languages : en
Pages : 472
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Book Description
This book discusses recent developments in the study of chemical processes and equilibria in the marine environment and in the air/water and water/sediment interfaces. The chemical cycle of carbon as well as the effect of organic substances on the speciation and distribution of inorganic and organometallic substances are extensively discussed. Much of the recent progress in the area is the direct result of advanced analytical technologies and chemometric applications which are highlighted in the book.
Author: Asanka Wijekoon
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 274
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Book Description
High performance liquid chromatography is a powerful analytical technique employed to separate analyte mixtures. Since biomedical sample mixtures contain a very diverse range of compounds; one chromatographic mode is usually unable to separate all components of the mixture. Therefore, there is an increasing need for faster and efficient separations with a broad range of selectivity. To attain better selectivity and efficient separation, and to improve the versatility of the chromatographic column, a new way of preparation of multifunctional stationary phases, suitable for use in multiple chromatographic separation modes, is described. The preparation of multifunctional stationary phases takes advantage of Schiff base chemistry in two different synthetic routes. The first route involves reductive coupling of amino bonded silica with aldehyde containing ligands. The coupling of aldehyde bonded silica with amine containing ligands is the other reaction route. Aldehyde terminated silica is a versatile reactive platform that enables the synthesis of a wide range of stationary phases by attaching amine containing ligands that have diverse functionality. Here the preparation of aldehyde terminated silica was achieved by bonding aldehyde silane to the silica surface. Preparation of aldehyde silane is a novel approach and a successful synthetic scheme of making acetalated aromatic aldehyde silane (AAS- Aldehyde group in the silane has been protected by acetalation) and aromatic aldehyde silane (AS) is invented. The studies confirmed that the synthesized multifunctional chromatographic stationary phases were capable of operating in ion exchange mode, reversed phase mode and hydrophilic interaction liquid chromatographic mode. The studies further concluded that those surfaces have good selectivity for the separation of small polar and charge molecules. The application of the well established Schiff base reaction allowed the incorporation 2H to the bonded phases and is utilized to study the motional dynamics of the bonded ligands in the surface at different temperatures in the presence of a broad range of solvent systems by using 2H wide line NMR spectroscopy. The investigation provides information about the motional dynamic heterogeneity of the bonded ligands, which reflects the existence of surface heterogeneity of the surface.
Author: Eli Grushka
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 256
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Author: Steven Douglas Fazio
Publisher:
ISBN:
Category : Chromatographic analysis
Languages : en
Pages : 392
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