Diagnostic Virology Protocols PDF Download
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Author: John R. Stephenson
Publisher: Springer Science & Business Media
ISBN: 9780896034792
Category : Medical
Languages : en
Pages : 388
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Book Description
A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Author: John R. Stephenson
Publisher: Springer Science & Business Media
ISBN: 9780896034792
Category : Medical
Languages : en
Pages : 388
Get Book Here
Book Description
A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Author: P.A. Bachmann
Publisher: Springer Science & Business Media
ISBN: 3642689493
Category : Medical
Languages : en
Pages : 332
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Book Description
The contributions to this book derived from the Seventh Munich Symposium on Microbiology on June 3 and 4, 1981, which was organized by the WHO Centre for Collection and Evaluation of Data on Comparative Virology at the Institute of Medical Microbiology, Infectious and Epidemic Diseases, University of Munich, Federal Repub lic of Germany. One of our principal purposes was to establish a forum at which the comparative aspects of questions of current interest in the field of medical virology could be discussed. In addition to the presentation of recent fmdings in microbiology, our overall aim was to crystallize trends and indicate new directions for future research activities. This book is a topical review of "New Horizons in Diagnostic Virology." Every one interested in virology is aware of the tremendous progress made in viral diagnostic techniques during recent years and the growing importance of viral diagnosis in human and veterinary medicine. There is yet another step that diagnostic virology has to take: the introduction on a routine basis of methods of molecular biology into the viral diagnostic laboratory. The application of monoclonal antibodies and techniques for the chemical and biological identification of proteins, carbohydrates, and enzymes are discussed, as is the introduction of techniques for the characterization of nucleic acids in viral diag nosis.
Author: Reeti Khare
Publisher: John Wiley & Sons
ISBN: 155581994X
Category : Medical
Languages : en
Pages : 460
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Book Description
The explosion in clinical testing has been especially rapid in virology, where emerging viruses and growing numbers of viral infections are driving advances. The Guide to Clinical and Diagnostic Virology offers a digestible view of the breadth and depth of information related to clinical virology, providing a practical, working knowledge of the wide array of viruses that cause human disease. Introductory chapters cover the basics of clinical virology and laboratory diagnosis of infections, including virus structure, life cycle, transmission, taxonomy, specimen types and handling, and a comparison of assays used for detection. Detailed sections on important topics include Viral pathogens and their clinical presentations Diagnostic assays and techniques, including culture-based, immunological, and molecular Prevention and management of viral infections, with guidance on biosafety, vaccines, and antiviral therapies The regulatory environment for laboratory testing, including regulatory requirements and assay performance and interpretation Critical concepts are carefully curated and concisely summarized and presented with detailed illustrations that aid comprehension, along with important highlights and helpful hints. These features, plus question sections that reinforce significant ideas and key concepts, make this an invaluable text for anyone looking for an accessible route through clinical and diagnostic virology. Laboratory technologists, medical students, infectious disease and microbiology fellows, pathology residents, researchers, and everyone involved with viruses in the clinical setting will find the Guide to Clinical and Diagnostic Virology an excellent text as well as companion to clinical virology references.
Author: Gueh Djen Hsiung
Publisher: Yale University Press
ISBN: 9780300058451
Category : Medical
Languages : en
Pages : 420
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Book Description
This generously illustrated book, the fourth edition of the highly acclaimed Diagnostic Virology, is a handbook for the recognition and characterization of virus-induced cellular changes seen by light microscopy, as well as virus morphology seen by electron microscopy. The authors cover all aspects of the subject, from specimen collection and laboratory safety to virus isolation and identification and cell culture. This edition includes a new chapter on the human retroviruses, especially relevant since the onset of the AIDS epidemic, and expands the chapters on the arboviruses and hepatitis viruses.
Author: Goura Kudesia
Publisher: Cambridge University Press
ISBN: 0521694671
Category : Medical
Languages : en
Pages : 281
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Book Description
Practical text provides quick access to key diagnostic features of each virus encountered in clinical practice and their management.
Author: Yi-Wei Tang
Publisher: Springer
ISBN: 3319951114
Category : Medical
Languages : en
Pages : 760
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Book Description
In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions.
Author: M.S. McNulty
Publisher: Springer Science & Business Media
ISBN: 9400960395
Category : Medical
Languages : en
Pages : 221
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Book Description
The traditional approach to diagnosis of virus infectians by isolation of the causative virus is usually both slow and expensive. More recently, the emphasis has been on the direct detection of viruses or viral antigens in clinical specimens. This can be done using established techniques such as immunofluorescence or electron microscopy, or by newly developed biochemical methods. The purpose of this meeting was to review these and other developments in the laboratory diagnosis of virus infections. We would like to thank all those who contributed to the success of this meeting. In particular, we are grateful to the CEC for financial sponsorship, to Professor C. Dow, Director of the Veterinary Research Laboratories, for help in organising the meeting, and to Miss B. Hamilton for her excellent typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS Page Preface V VIRAL DIAGNOSIS BY TIMMUNOFLUORESCENCE P. S. Gardner APPLICATIONS OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL DIAGNOSIS M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS USING IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC CATTLE BY AN INDIRECT IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL DIAGNOSIS BY ELECTRON MICROSCOPY J. D. Almeida 47 CAPTURE ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND IDENTIFICATION OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF CATTLE M. S. Campo 72 DETECTION OF VIRAL NUCLEIC ACIDS IN CELL CULTURES .
Author: P.B. Raghavendra
Publisher: Academic Press
ISBN: 0128136804
Category : Science
Languages : en
Pages : 300
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Book Description
Advances in Cell and Molecular Diagnostics brings the scientific advances in the translation and validation of cellular and molecular discoveries in medicine into the clinical diagnostic setting. It enumerates the description and application of technological advances in the field of cellular and molecular diagnostic medicine, providing an overview of specialized fields, such as biomarker, genetic marker, screening, DNA-profiling, NGS, cytogenetics, transcriptome, cancer biomarkers, prostate specific antigen, and biomarker toxicologies. In addition, it presents novel discoveries and clinical pathologic correlations, including studies in oncology, infectious diseases, inherited diseases, predisposition to disease, and the description or polymorphisms linked to disease states. This book is a valuable resource for oncologists, practitioners and several members of the biomedical field who are interested in understanding how to apply cutting-edge technologies into diagnostics and healthcare. - Encompasses the current scientific advances in the translation and validation of cellular and molecular discoveries into the clinical diagnostic setting - Explains the application of cellular and molecular diagnostics methodologies in clinical trials - Focuses on translating preclinical tests to the bedside in order to help readers apply the most recent technologies to healthcare
Author: Frank J. Fenner
Publisher: Academic Press
ISBN: 1483257819
Category : Technology & Engineering
Languages : en
Pages : 673
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Book Description
Veterinary Virology deals with basic biomedical virology and the clinical discipline of infectious diseases. The book discusses the principles of virology as effecting future developments in the search for preventive and management of infectious diseases in animals, whether singly or as a whole herd or flock. Part I explains the principles of animal virology including the structure, composition, classification, nomenclature, cultivation, and assay of viruses. This part also discusses viral genetics, replication, and evolution (including mutation and genetic engineering). The book also reviews the pathogenesis of viruses, host resistance and susceptibility, as well as the mechanisms of persistent infections and tumor induction. Part II deals with viruses found in domestic animals; this part also explains in detail the properties, replication methods, pathogenesis, immunity, diagnosis, and control of some common viruses. The book discusses some other families of viruses of which no members are yet known as to have caused serious or important diseases in animals. Veterinarians, immunologists, virologists, molecular researchers, students, and academicians in the discipline of virology and cellular biology, as well as livestock owners will find this book helpful.
Author: Douglas D. Richman
Publisher: John Wiley & Sons
ISBN: 1555819435
Category : Medical
Languages : en
Pages : 1489
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Book Description
The essential reference of clinical virology Virology is one of the most dynamic and rapidly changing fields of clinical medicine. For example, sequencing techniques from human specimens have identified numerous new members of several virus families, including new polyomaviruses, orthomyxoviruses, and bunyaviruses. Clinical Virology, Fourth Edition, has been extensively revised and updated to incorporate the latest developments and relevant research. Chapters written by internationally recognized experts cover novel viruses, pathogenesis, epidemiology, diagnosis, treatment, and prevention, organized into two major sections: Section 1 provides information regarding broad topics in virology, including immune responses, vaccinology, laboratory diagnosis, principles of antiviral therapy, and detailed considerations of important organ system manifestations and syndromes caused by viral infections. Section 2 provides overviews of specific etiologic agents and discusses their biology, epidemiology, pathogenesis of disease causation, clinical manifestations, laboratory diagnosis, and management. Clinical Virology provides the critical information scientists and health care professionals require about all aspects of this rapidly evolving field.
