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Author: Benjamin R. Arenkiel
Publisher: Humana
ISBN: 9781493943418
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.
Author: Benjamin R. Arenkiel
Publisher: Humana
ISBN: 9781493943418
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume seeks to familiarize readers with a diverse range of technologies and approaches for probing neuron and circuit architecture, and, when possible, to attach detailed protocols to help guide readers toward practical application. From classical lipophilic dye and conjugated lectin tracing techniques, to electrophysiological, in vivo imaging, viral tract tracing, and emerging genetic methods to mark, manipulate, and monitor neural circuits, Neural Tracing Methods: Tracing Neurons and Their Connections includes reference to an arsenal of tools and technologies currently being implemented in model systems ranging from flies to mice. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, Neural Tracing Methods: Tracing Neurons and Their Connections collects a comprehensive compilation of chapters authored by inventors and expert users, that describes state-of-the-art neuronal tracing and functional analysis methods in order to aid researchers in continuing this vital pathway of study.
Author: Laszlo Zaborszky
Publisher: Springer Science & Business Media
ISBN: 0387289429
Category : Medical
Languages : en
Pages : 703
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Book Description
The first two editions of this title had a tremendous impact in neuroscience. Between the Second edition in 1989 and today, there has been an explosion of information in the field, including advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from tissue culture to neurophysiology to functional MRI techniques.
Author: Laszlo Zaborszky
Publisher: Springer Science & Business Media
ISBN: 1475720556
Category : Medical
Languages : en
Pages : 429
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Book Description
This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Author: Shuyang Zhang
Publisher:
ISBN:
Category :
Languages : en
Pages : 51
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Book Description
Neuronal tracing methods are essential tools to understand the fundamental architecture of the neural circuits and their connection to the overall functional behavior of the brain. Various viral vectors have been developed to achieve cell-type specific and directional specific labeling of the neuronal connections. The incorporation of the viral tracers with optogenetics techniques can further provide precise control over the viral propagation. Herein, a novel approach to guide the infectious pattern of the Rabies Virus (RV) retrograde tracer with light is described. The key concept is to use Baculovirus (BV) as a helper virus to deliver all the functional components prior to the rabies infection. These functional components include the optogenetic tool parts, a fluorescent CRE reporter to mark RV infected cells, and other genes necessary for RV spread. GoldenGate DNA assembly method was used to efficiently construct three large BV expression cassettes in bacteria plasmids that can be transposed into the baculovirus genome in a single TN7 transposon recombination step. The actual imaging of BV and RV infected cells and stimulation of optogenetics tools for the spread of RV can be achieved with CMOS sensor and OLED display panels. By integrating these commercially available electronics with proper microcontrollers, a synchronized communication protocol for optogenetic stimulation, image capturing, and file transferring was established in a lensless imaging setup. The OLED and CMOS sensor's large field of view and low cost allows this tracing method to scale many orders of magnitude greater than using microscopy-based methods.
Author: Institute of Medicine
Publisher: National Academies Press
ISBN: 0309044979
Category : Computers
Languages : en
Pages : 180
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Book Description
Significant advances in brain research have been made, but investigators who face the resulting explosion of data need new methods to integrate the pieces of the "brain puzzle." Based on the expertise of more than 100 neuroscientists and computer specialists, this new volume examines how computer technology can meet that need. Featuring outstanding color photography, the book presents an overview of the complexity of brain research, which covers the spectrum from human behavior to genetic mechanisms. Advances in vision, substance abuse, pain, and schizophrenia are highlighted. The committee explores the potential benefits of computer graphics, database systems, and communications networks in neuroscience and reviews the available technology. Recommendations center on a proposed Brain Mapping Initiative, with an agenda for implementation and a look at issues such as privacy and accessibility.
Author: M.-Marsel Mesulam
Publisher: John Wiley & Sons
ISBN:
Category : Medical
Languages : en
Pages : 280
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Book Description
Surveys methods for tracing neural connections with the tracer horseradish peroxidase. Discusses light-microscopic tracing following extracellular injections of tracer. Describes electron microscopic applications of horseradish peroxidase histochemistry for tracing neural connections, and compares various histochemical methods. Also discusses tracing connections after intracellular injections of HRP into physiologically identified neurons. Fully treats the relevant biological principles which guide each application
Author:
Publisher: Elsevier
ISBN: 0080862136
Category : Medical
Languages : en
Pages : 657
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Book Description
Neurobiology of the Locus Coeruleus
Author: Masahito Yamagata
Publisher: Frontiers Media SA
ISBN: 2889711498
Category : Science
Languages : en
Pages : 294
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Book Description
Author: IEEE Staff
Publisher:
ISBN: 9781665429474
Category :
Languages : en
Pages :
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Book Description
The IEEE International Symposium on Biomedical Imaging (ISBI) is the premier forum for the presentation of technological advances in theoretical and applied biomedical imaging ISBI 2021 will be the 18th meeting in this series The previous meetings have played a leading role in facilitating interaction between researchers in medical and biological imaging The 2021 meeting will continue this tradition of fostering cross fertilization among different imaging communities and contributing to an integrative approach to biomedical imaging across all scales of observation
Author: Michael Schulte-Mecklenbeck
Publisher: Routledge
ISBN: 1351662767
Category : Psychology
Languages : en
Pages : 409
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Book Description
A Handbook of Process Tracing Methods demonstrates how to better understand decision outcomes by studying decision processes, through the introduction of a number of exciting techniques. Decades of research have identified numerous idiosyncrasies in human decision behavior, but some of the most recent advances in the scientific study of decision making involve the development of sophisticated methods for understanding decision process—known as process tracing. In this volume, leading experts discuss the application of these methods and focus on the best practices for using some of the more popular techniques, discussing how to incorporate them into formal decision models. This edition has been expanded and thoroughly updated throughout, and now includes new chapters on mouse tracking, protocol analysis, neurocognitive methods, the measurement of valuation, as well as an overview of important software packages. The volume not only surveys cutting-edge research to illustrate the great variety in process tracing techniques, but also serves as a tutorial for how the novice researcher might implement these methods. A Handbook of Process Tracing Methods will be an essential read for all students and researchers of decision making.