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Author: Timothy-Neil Trinidad Tiambeng
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Cardiac troponin I (cTnI) is a universally-accepted, clinical biomarker for the diagnosis of acute myocardial infarction (a.k.a., heart attack). cTnI is released into the bloodstream following cardiovascular injury where it circulates with low abundance (ng/ml) and in myriad proteoforms (e.g., phosphorylated, acetylated, oxidized, and truncated). Top-down mass spectrometry (MS)-based proteomics has emerged as a powerful technology for characterizing and quantifying proteoforms arising from post-translational modifications, alternative splicing, and sequence variations at the intact protein level. However, the large dynamic range of the blood proteome makes the MS-detection of low-abundance biomarker proteins extremely challenging. Thus, protein enrichment strategies are necessary to specifically capture and enrich low-abundance proteins from complex mixtures prior to MS-analysis. In chapter 1, an introduction is given regarding the use of top-down mass spectrometry in health and disease cardiac protein biomarkers, and the need for front-end enrichment methods. In chapter 2, I describe the development of an integrated "nanoproteomics" method coupling peptide-functionalized superparamagnetic nanoparticles (NPs) with top-down MS for the enrichment and comprehensive analysis of cTnI proteoform monomers, directly from serum for the first time. Chapter 3 utilizes the NP platform developed in chapter 2 to purify and enable the native MS analysis of intact, non-recombinant cardiac troponin complexes (cTnT-cTnI-cTnC; 77 kDa) from human heart tissues, with native MS coupled online with size exclusion chromatography (SEC), thereby enabling the native MS analysis of cardiac troponin complexes for the first time. Chapter 4 describes future directions with using nanoproteomics for the analysis of clinical acute myocardial infarction samples, and the structural analysis of cardiac troponin complexes. Finally, the thesis is concluded with two Appendices containing step-by-step protocols and methods for the analysis of cardiac troponin proteoforms by top-down mass spectrometry, and size exclusion chromatography strategies combined with MASH Explorer for targeted proteoform characterization. Overall, the work described here demonstrates how nanoproteomics can provide proteoform- and protein complex- resolved details of cardiac troponin to elucidate the molecular mechanisms underlying health and disease.
Author: Timothy-Neil Trinidad Tiambeng
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Cardiac troponin I (cTnI) is a universally-accepted, clinical biomarker for the diagnosis of acute myocardial infarction (a.k.a., heart attack). cTnI is released into the bloodstream following cardiovascular injury where it circulates with low abundance (ng/ml) and in myriad proteoforms (e.g., phosphorylated, acetylated, oxidized, and truncated). Top-down mass spectrometry (MS)-based proteomics has emerged as a powerful technology for characterizing and quantifying proteoforms arising from post-translational modifications, alternative splicing, and sequence variations at the intact protein level. However, the large dynamic range of the blood proteome makes the MS-detection of low-abundance biomarker proteins extremely challenging. Thus, protein enrichment strategies are necessary to specifically capture and enrich low-abundance proteins from complex mixtures prior to MS-analysis. In chapter 1, an introduction is given regarding the use of top-down mass spectrometry in health and disease cardiac protein biomarkers, and the need for front-end enrichment methods. In chapter 2, I describe the development of an integrated "nanoproteomics" method coupling peptide-functionalized superparamagnetic nanoparticles (NPs) with top-down MS for the enrichment and comprehensive analysis of cTnI proteoform monomers, directly from serum for the first time. Chapter 3 utilizes the NP platform developed in chapter 2 to purify and enable the native MS analysis of intact, non-recombinant cardiac troponin complexes (cTnT-cTnI-cTnC; 77 kDa) from human heart tissues, with native MS coupled online with size exclusion chromatography (SEC), thereby enabling the native MS analysis of cardiac troponin complexes for the first time. Chapter 4 describes future directions with using nanoproteomics for the analysis of clinical acute myocardial infarction samples, and the structural analysis of cardiac troponin complexes. Finally, the thesis is concluded with two Appendices containing step-by-step protocols and methods for the analysis of cardiac troponin proteoforms by top-down mass spectrometry, and size exclusion chromatography strategies combined with MASH Explorer for targeted proteoform characterization. Overall, the work described here demonstrates how nanoproteomics can provide proteoform- and protein complex- resolved details of cardiac troponin to elucidate the molecular mechanisms underlying health and disease.
Author: Vijay Nambi
Publisher: Elsevier Health Sciences
ISBN: 0323548369
Category : Medical
Languages : en
Pages : 400
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Book Description
Get a quick, expert overview of the ways in which biomarkers can be used to assess and guide the management of cardiovascular disease in the clinical setting. This concise, clinically-focused resource by Dr. Vijay Nambi consolidates today’s available information on this rapidly changing topic into one convenient resource, making it an ideal, easy-to-digest reference for cardiology practitioners, fellows, and residents. Covers lab standards and statistical interpretation of biomarkers with a clinical focus. Discusses relevant conditions such as hypertension and diabetes as key markers of injury and prognosis. Includes current information on biomarkers to assess and guide the management of heart failure, acute coronary syndrome, chest pain, shortness of breath, and more. Concludes the book with a timely chapter on how biomarkers may guide cardiologists in the future.
Author: Rhian M. Touyz
Publisher: Springer
ISBN: 3030164810
Category : Medical
Languages : en
Pages : 503
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Book Description
This textbook focuses on the vascular biology and physiology that underlie vascular disorders in clinical medicine. Vascular biomedicine is a rapidly growing field as new molecular mechanisms of vascular health and disease are unraveled. Many of the major cardiovascular diseases including coronary artery disease, heart failure, stroke and vascular dementia are diseases of the vasculature. In addition vascular injury underpins conditions like kidney failure and cardiovascular complications of diabetes. This field is truly multidisciplinary involving scientists in many domains such as molecular and vascular biology, cardiovascular physiology and pharmacology and immunology and inflammation. Clinically, specialists across multiple disciplines are involved in the management of patients with vascular disorders, including cardiologists, nephrologists, endocrinologists, neurologists and vascular surgeons. This book covers a wide range of topics and provides an overview of the discipline of vascular biomedicine without aiming at in-depth reviews, but rather offering up-to-date knowledge organized in concise and structured chapters, with key points and pertinent references. The structure of the content provides an integrative and translational approach from basic science (e.g. stem cells) to clinical medicine (e.g. cardiovascular disease). The content of this book is targeted to those who are new in the field of vascular biology and vascular medicine and is ideal for medical students, graduate and postgraduate students, clinical fellows and academic clinicians with an interest in the vascular biology and physiology of cardiovascular disease and related pathologies.
Author: Kewal K. Jain
Publisher: Springer Science & Business Media
ISBN: 1607616858
Category : Medical
Languages : en
Pages : 509
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Book Description
Of the thousands of biomarkers that are currently being discovered, relatively few are being validated for further applications, and the potential of a biomarker can be quite difficult to evaluate. To aid in this imperative research, Dr. Kewal K. Jain’s Handbook of Biomarkers thoroughly describes many different types of biomarkers and their discovery using various "-omics" technologies, such as proteomics and metabolomics, along with the background information needed for the evaluation of biomarkers as well as the essential procedures for their validation and use in clinical trials. With biomarkers described first according to technologies and then according to various diseases, this detailed book features the key correlations between diseases and classifications of biomarkers, which provides the reader with a guide to sort out current and future biomarkers. Comprehensive and cutting-edge, The Handbook of Biomarkers serves as a vital guide to furthering our understanding of biomarkers, which, by facilitating the combination of therapeutics with diagnostics, promise to play an important role in the development of personalized medicine, one of the most important emerging trends in healthcare today.
Author: Chi-san Wu
Publisher: Elsevier
ISBN: 0080529399
Category : Science
Languages : en
Pages : 663
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Book Description
Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Key Features* Evaluate and select columns with confidence for specific applications* Optimize separations and improve the ruggedness of analytical methods* Extend the service time of a column* Establish a quality-control program to ensure consistency in column performance* Avoid the expense of column damage or purchases that do not give the expected results
Author: Yoshinori Yoshida
Publisher:
ISBN: 9781071614846
Category : Cardiovascular system
Languages : en
Pages : 304
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Book Description
This volume provides methodologies for ES and iPS cell technology on the study of cardiovascular diseases. Chapters guide readers through protocols on cardiomyocyte generation from pluripotent stem cells, physiological measurements, bioinformatic analysis, gene editing technology, and cell transplantation studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Pluripotent Stem-Cell Derived Cardiomyocytes aims to help researchers set up experiments using pluripotent stem cell-derived cardiac cells.
Author: Liangliang Sun
Publisher: Humana
ISBN: 9781071623275
Category : Science
Languages : en
Pages : 0
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Book Description
This volume discusses the latest mass spectrometry (MS)-based technologies for proteoform identification, characterization, and quantification. Some of the topics covered in this book include sample preparation, proteoform separation, proteoform gas-phase fragmentation, and bioinformatics tools for MS data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Proteoform Identification: Methods and Protocols is a valuable resource for researchers in both academia and the biopharmaceutical industry who are interested in proteoform analysis using MS.
Author: Jozef Samaj
Publisher: Springer Science & Business Media
ISBN: 3540726179
Category : Science
Languages : en
Pages : 397
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Book Description
Plant Proteomics highlights rapid progress in this field, with emphasis on recent work in model plant species, sub-cellular organelles, and specific aspects of the plant life cycle such as signaling, reproduction and stress physiology. Several chapters present a detailed look at diverse integrated approaches, including advanced proteomic techniques combined with functional genomics, bioinformatics, metabolomics and molecular cell biology, making this book a valuable resource for a broad spectrum of readers.
Author: Yoshinori Mine
Publisher: CRC Press
ISBN: 1420028839
Category : Technology & Engineering
Languages : en
Pages : 678
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Book Description
Reports of the beneficial health effects of some peptides have begun to make their way into the scientific literature. Peptides can act as immunomodulators, and have been shown to have a positive influence on calcium absorption, and on regulation of serum cholesterol. A number of peptides may also possess antimicrobial properties that enhance the b
Author: Bansi D. Malhotra
Publisher: William Andrew
ISBN: 0128135158
Category : Technology & Engineering
Languages : en
Pages : 334
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Book Description
Nanomaterials for Biosensors: Fundamentals and Applications provides a detailed summary of the main nanomaterials used in biosensing and their application. It covers recent developments in nanomaterials for the fabrication of biosensor devices for healthcare diagnostics, food freshness and bioprocessing. The various processes used for synthesis and characterization of nanostructured materials are examined, along with the design and fabrication of bioelectronic devices using nanostructured materials as building blocks. Users will find the fundamentals of the main nanomaterials used in biosensing, helping them visualize a systematic and coherent picture of how nanomaterials are used in biosensors. The book also addresses the role of bio-conjugation of nanomaterials in the construction of nano-biointerfaces for application in biosensors. Such applications, including metal nanoparticles, metal oxide nanoparticles, nanocomposites, carbon nanotubes, conducting polymers and plasmonic nanostructures in biosensing are discussed relative to each nanomaterial concerned. Finally, recent advancements in protein functionalized nanomaterials for cancer diagnostics and bio-imaging are also included. Provides a detailed study on how nanomaterials are used to enhance sensing capabilities in biosensors Explains the properties, characterization methods and preparation techniques of the nanomaterials used in biosensing Arranged in a material-by-material way, making it clear how each nanomaterial should be used
