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Author: Steven A. Toms
Publisher: Humana Press
ISBN: 9781617793202
Category : Science
Languages : en
Pages : 322
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Book Description
As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues. The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular BiologyTM, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls. Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.
Author: Steven A. Toms
Publisher: Humana Press
ISBN: 9781617793202
Category : Science
Languages : en
Pages : 322
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Book Description
As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues. The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular BiologyTM, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls. Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.
Author: Carlos A. Guzmán
Publisher: Springer Science & Business Media
ISBN: 1441911324
Category : Medical
Languages : en
Pages : 276
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Book Description
Pharmaceutical Biotechnology is a unique compilation of reviews addressing frontiers in biologicals as a rich source for innovative medicines. This book fulfills the needs of a broad community of scientists interested in biologicals from diverse perspectives—basic research, biotechnology, protein engineering, protein delivery, medicines, pharmaceuticals and vaccinology. The diverse topics range from advanced biotechnologies aimed to introduce novel, potent engineered vaccines of unprecedented efficacy and safety for a wide scope of human diseases to natural products, small peptides and polypeptides engineered for discrete prophylaxis and therapeutic purposes. Modern biologicals promise to dramatically expand the scope of preventive medicine beyond the infectious disease arena into broad applications in immune and cancer treatment, as exemplified by anti-EGFR receptors antibodies for the treatment of breast cancer. The exponential growth in biologicals such as engineered proteins and vaccines has been boosted by unprecedented scientific breakthroughs made in the past decades culminating in an in-depth fundamental understanding of the scientific underpinnings of immune mechanisms together with knowledge of protein and peptide scaffolds that can be deliberately manipulated. This has in turn led to new strategies and processes. Deciphering the human, mammalian and numerous pathogens’ genomes provides opportunities that never before have been available—identification of discrete antigens (genomes and antigenomes) that lend themselves to considerably improved antigens and monoclonal antibodies, which with more sophisticated engineered adjuvants and agonists of pattern recognition receptors present in immune cells, deliver unprecedented safety and efficacy. Technological development such a nanobiotechnologies (dendrimers, nanobodies and fullerenes), biological particles (viral-like particles and bacterial ghosts) and innovative vectors (replication-competent attenuated, replication-incompetent recombinant and defective helper-dependent vectors) fulfill a broad range of cutting-edge research, drug discovery and delivery applications. Most recent examples of breakthrough biologicals include the human papilloma virus vaccine (HPV, prevention of women genital cancer) and the multivalent Pneumoccocal vaccines, which has virtually eradicated in some populations a most prevalent bacterial ear infection (i.e., otitis media). It is expected that in the years to come similar success will be obtained in the development of vaccines for diseases which still represent major threats for human health, such as AIDS, as well as for the generation of improved vaccines against diseases like pandemic flu for which vaccines are currently available. Furthermore, advances in comparative immunology and innate immunity revealed opportunities for innovative strategies for ever smaller biologicals and vaccines derived from species such as llama and sharks, which carry tremendous potential for innovative biologicals already in development stages in many pharmaceutical companies. Such recent discoveries and knowledge exploitations hold the promise for breakthrough biologicals, with the coming decade. Finally, this book caters to individuals not directly engaged in the pharmaceutical drug discovery process via a chapter outlining discovery, preclinical development, clinical development and translational medicine issues that are critical the drug development process. The authors and editors hope that this compilation of reviews will help readers rapidly and completely update knowledge and understanding of the frontiers in pharmaceutical biotechnologies.
Author: Sonam Chawla
Publisher: Springer Nature
ISBN: 9819917182
Category : Science
Languages : en
Pages : 418
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Book Description
The book showcases the newest research on smart nanoplatforms responding to “hypoxia”; an omnipresent pathological feature of many modern-day diseases and its potential applications. The book demonstrates the versatility of hypoxia targeted smart nanoplatforms in disease management in diverse pathological settings presented in chapters contributed by various experts in the field. The themes in the book touch upon critical facets to address pathological hypoxia such as nanomaterials enhancing oxygenation, hypoxia responsive nanoformulations which deliver the drug directly to the hypoxic site, limited toxicity and enhanced efficacy of smart nanoformulations to counter molecular consequences of hypoxia, and theranostics combining hypoxia detection systems with drug delivery systems. This book disseminates the current implications of smart nanomaterials in disease management that can be exploited by scholars, researchers, and pharma industries to develop, manufacture, and commercialize hypoxia targeted diagnostics and therapeutics.
Author: Anil Kumar Anal
Publisher: CRC Press
ISBN: 1351643630
Category : Medical
Languages : en
Pages : 144
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Book Description
This book deals with a subject of high interest and importance in all sectors, including biomedical, food, agriculture, energy, and environment. Biological systems are essential in nanotechnology, and many new applications are being developed by mimicking the natural systems. Approaching these topics from an engineering perspective, the book offers insight on the details of nanoscale fabrication processes as well as cell biology. The basics of biology and chemistry, with a focus on how to engineer the behavior of molecules at the nanoscale, are also explored and analyzed. The aim of the text is to provide the reader with broader knowledge of biological methods for signal transduction and molecular recognitions systems and how they can be replicated in bio-sensing applications. The reader will learn the basic structures and interactions of biomacromolecules for developing biocompatible and eco-friendly devices.
Author:
Publisher: Academic Press
ISBN: 0128052112
Category : Science
Languages : en
Pages : 400
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Book Description
The Advances in Protein Chemistry and Structural Biology series is an essential resource for protein chemists. Each volume brings forth new information about protocols and analysis of proteins, with each thematically organized volume guest edited by leading experts in a broad range of protein-related topics. Provides cutting-edge developments in protein chemistry and structural biology Chapters are written by authorities in their field Targeted to a wide audience of researchers, specialists, and students
Author: Timothy-Neil Trinidad Tiambeng
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Cardiac troponin I (cTnI) is a universally-accepted, clinical biomarker for the diagnosis of acute myocardial infarction (a.k.a., heart attack). cTnI is released into the bloodstream following cardiovascular injury where it circulates with low abundance (ng/ml) and in myriad proteoforms (e.g., phosphorylated, acetylated, oxidized, and truncated). Top-down mass spectrometry (MS)-based proteomics has emerged as a powerful technology for characterizing and quantifying proteoforms arising from post-translational modifications, alternative splicing, and sequence variations at the intact protein level. However, the large dynamic range of the blood proteome makes the MS-detection of low-abundance biomarker proteins extremely challenging. Thus, protein enrichment strategies are necessary to specifically capture and enrich low-abundance proteins from complex mixtures prior to MS-analysis. In chapter 1, an introduction is given regarding the use of top-down mass spectrometry in health and disease cardiac protein biomarkers, and the need for front-end enrichment methods. In chapter 2, I describe the development of an integrated "nanoproteomics" method coupling peptide-functionalized superparamagnetic nanoparticles (NPs) with top-down MS for the enrichment and comprehensive analysis of cTnI proteoform monomers, directly from serum for the first time. Chapter 3 utilizes the NP platform developed in chapter 2 to purify and enable the native MS analysis of intact, non-recombinant cardiac troponin complexes (cTnT-cTnI-cTnC; 77 kDa) from human heart tissues, with native MS coupled online with size exclusion chromatography (SEC), thereby enabling the native MS analysis of cardiac troponin complexes for the first time. Chapter 4 describes future directions with using nanoproteomics for the analysis of clinical acute myocardial infarction samples, and the structural analysis of cardiac troponin complexes. Finally, the thesis is concluded with two Appendices containing step-by-step protocols and methods for the analysis of cardiac troponin proteoforms by top-down mass spectrometry, and size exclusion chromatography strategies combined with MASH Explorer for targeted proteoform characterization. Overall, the work described here demonstrates how nanoproteomics can provide proteoform- and protein complex- resolved details of cardiac troponin to elucidate the molecular mechanisms underlying health and disease.
Author: Kewal K. Jain
Publisher: Humana Press
ISBN: 1493969668
Category : Technology & Engineering
Languages : en
Pages : 661
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Book Description
Nanomedicine is defined as the application of nanobiotechnology in clinical medicine, which is currently being used to research the pathomechanism of disease, refine molecular diagnostics, and aid in the discovery, development, and delivery of drugs. In The Handbook of Nanomedicine, Third Edition, Prof. Kewal K. Jain updates, reorganizes, and replaces information in the comprehensive second edition in order to capture the most recent advances in this dynamic field. Important components of nanomedicine such as drug delivery via nanobiotechnology and nanopharmaceuticals as well as nanooncology, where the greatest number of advances are occurring, are covered extensively. As this text is aimed at nonmedical scientists, pharmaceutical personnel, as well as physicians, descriptions of the technology involved and other medical terminology are kept as clear and simple as possible. In depth and cutting-edge, The Handbook of Nanomedicine, Third Edition informs its readers of the ever-growing field of nanomedicine, destined to play a significant role in the future of healthcare.
Author: Rob Burgess
Publisher: CRC Press
ISBN: 9814303526
Category : Medical
Languages : en
Pages : 514
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Book Description
This book comprehensively covers a broad range of therapeutic and diagnostic applications of nanotechnology, providing descriptions of cutting-edge discoveries along with historical perspectives. The text focuses on nanomaterials and nanoparticles, the sectors that hold the most promise for the future of medicine. The author look at how nanotechnol
Author: Malay K. Das
Publisher: Springer Nature
ISBN: 9811562555
Category : Science
Languages : en
Pages : 634
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Book Description
This book reviews the application of Nanobiotechnology in the development of Nanomedicine, while also discussing the latest trends and challenges in the clinical translation of Nanomedicine. Nanomedicine refers to the application of Nanotechnology to medicine and holds tremendous potential for achieving improved efficiency, bioavailability, dose response, personalized medicine and enhanced safety as compared to conventional medicines. The book first introduces readers to the basic concepts of Nanomedicine, and to technological advances in and applications of Nanotechnology in treatment, diagnosis, monitoring, and drug delivery. In turn, it reviews the current status of multi-functionalization strategies for using Nanoparticles in the targeted delivery of therapeutic agents. The book’s third and final section focuses on the regulatory and safety challenges posed by Nanomedicine, including industry and regulatory agencies’ efforts to address them.
Author: Nicola Luigi Bragazzi
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
