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Author: Maria E. Gracheva
Publisher: Humana Press
ISBN: 9781493958092
Category : Science
Languages : en
Pages : 267
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Book Description
Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality. In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization. Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing. Written for the highly successful Methods in Molecular BiologyTM series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results. Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths.
Author: Maria E. Gracheva
Publisher: Humana Press
ISBN: 9781493958092
Category : Science
Languages : en
Pages : 267
Get Book Here
Book Description
Nanopores are vital biological features, described as tiny holes in cellular membranes used for recognition and transport of ions and molecules between compartments within the cell, as well as between the extracellular environment and the cell itself. Their study, ever growing in esteem, leads toward the promise of ultra-fast sequencing of DNA molecules with the ultimate goal of building a nanoscale device that will make rapid and cheap DNA sequencing a reality. In Nanopore-Based Technology, expert researchers in the forefront of the field explore the cutting-edge of nanopore technology for single molecule sensing, detection, and characterization. Divided into four convenient parts, this volume covers single molecule characterization techniques utilizing biological pores, methods for biomolecule characterization with nanoporous artificial membranes, computational studies of the biomolecule confined within the nanopore environment, as well as techniques that use novel materials in conjunction with nanopore sensing. Written for the highly successful Methods in Molecular BiologyTM series, this work provides the kind of detailed description and implementation advice that is crucial for achieving optimal results. Authoritative and state-of-the-art, Nanopore-Based Technology serves as an excellent representation of the present-day available techniques for biomolecule characterization with nanoporous membranes in order to guide researchers toward developing the next generation of technologies for fast and cheap DNA sequencing with practically no limitations on the read lengths.
Author: Joshua B. Edel
Publisher: William Andrew
ISBN: 143773474X
Category : Science
Languages : en
Pages : 189
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Book Description
Engineered Nanopores for Bioanalytical Applications is the first book to focus primarily on practical analytical applications of nanopore development. These nanoscale analytical techniques have exciting potential because they can be used in applications such as DNA sequencing, DNA fragment sizing, DNA/protein binding, and protein/protein binding.This book provides a solid professional reference on nanopores for readers in academia, industry and engineering and biomedical fields. In addition, the book describes the instrumentation, fabrication, and experimental methods necessary to carry out nanopore-based experiments for developing new devices. - Includes application case studies for detection, identification and analysis of biomolecules and related functional nanomaterials - Introduces the techniques of manufacturing solid state materials with functional nanopores - Explains the use of nanopores in DNA sequencing and the wider range of applications from environmental monitoring to forensics
Author: Branton Daniel
Publisher: World Scientific
ISBN: 9813270624
Category : Science
Languages : en
Pages : 216
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Book Description
This is an introductory text and laboratory manual to be used primarily in undergraduate courses. It is also useful for graduate students and research scientists who require an introduction to the theory and methods of nanopore sequencing. The book has clear explanations of the principles of this emerging technology, together with instructional material written by experts that describes how to use a MinION nanopore instrument for sequencing in research or the classroom.At Harvard University the book serves as a textbook and lab manual for a university laboratory course designed to intensify the intellectual experience of incoming undergraduates while exploring biology as a field of concentration. Nanopore sequencing is an ideal topic as a path to encourage students about the range of courses they will take in Biology by pre-emptively addressing the complaint about having to take a course in Physics or Maths while majoring in Biology. The book addresses this complaint by concretely demonstrating the range of topics — from electricity to biochemistry, protein structure, molecular engineering, and informatics — that a student will have to master in subsequent courses if he or she is to become a scientist who truly understands what his or her biology instrument is measuring when investigating biological phenomena.
Author: Jeffrey R. Comer
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
The pursuit of inexpensive DNA sequencing lies at the interface between nanotechnology and biotechnology0́4where silicon nanopores, nanoscale electrodes, and self-assembled molecular structures are as common as restriction enzymes, Ơ̐2uorescence detection, and genetic engineering. The motivation for this pursuit stems from the promise that personalized genomic information can make health care safer, faster, and more effective. Beyond the realm of human health, inexpensive sequencing is poised to have lasting impacts for the whole of biology. However, the integration of nanotechnology with biotechnology raises many difficult and interesting questions. Computer simulations can assist in answering these questions by providing a means to 0́−see0́+ nanoscale events that cannot be imaged by any experimental method. In the development of nanopore devices for DNA sequencing, computation has played a key role in revealing how DNA behaves in the high electric Ơ̐1eld and conƠ̐1ned geometry of a nanopore. In this dissertation, I describe my contribution to computational work for the development of nanopore-based sequencing technology. In simulations which have been corroborated by experiments, I have found that DNA adopts qualitatively different conformations in nanopores of different sizes and that nanopores of the appropriate geometry can be used to trap DNA and control its conformation. By developing a method that can provide millisecond-long current simulations with atomic resolution, I have determined conditions under which the sequence of this trapped DNA can be discriminated by ion current measurements.
Author: Monifa A.V. Fahie
Publisher: Humana
ISBN: 9781071608081
Category : Science
Languages : en
Pages : 231
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Book Description
This detailed collection explores techniques involved in the main strategies of nanopore sensing, such as translocation, analyte trapping, and interactions with external binding sites. Opening with a section on nanopore design and nanopore production, the book continues with parts devoted to various biological nanopores, nanopore engineering, and their uses in single molecule sensing, computational methods to study intrinsic nanopore behavior, characterizing the specific translocation activity of a vesicle particle through a nanopore, as well as the use of the technique droplet interface bilayer (DIB) in nanopore and membrane biophysical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nanopore Technology: Methods and Protocols, with its focus on nanopore technology and biomolecule characterization, will hold the interest of the biophysicists, biochemists, bioengineers, and molecular biologists who are working toward further understanding this key field of research.
Author: Christine Kranz
Publisher: Springer
ISBN: 3030118649
Category : Science
Languages : en
Pages : 308
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Book Description
Carbon nanomaterials have gained relevance in chem/bio sensing applications owing to their unique chemical, mechanical, electrical, and thermal characteristics. Written by leading experts in the field, this book discusses selected, state-of-the art carbon-based nanomaterials, including nanodiamonds, graphene nanodots, carbon nanopores, and nanocellulose. It presents examples of chem/bio sensing applications ranging from biomedical studies, such as DNA sequencing and neurotransmitter sensing, to heavy-metal detection in environmental monitoring scenarios, and reviews the unique properties of carbon-based nanomaterials with respect to targeted sensing applications. Further, it highlights exciting future applications. Providing comprehensive information for practitioners and scientists working in the field of carbon nanomaterial technologies and their application, it is also a valuable resource for advanced students of analytical chemistry, biochemistry, electrochemistry, materials science, and micro-/nanotechnology and -sensing.
Author: Kaitlyn Kean
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Continuous progress in the nanotechnology field has allowed for the emergence of powerful, nanopore-based detection technology. Solid-state nanopores were developed for next-generation sequencing and single-molecule detection. They are advantageous over their biological counterpart because they offer robustness, stability, tunable pore size and the ability to be integrated within a microfluidic device. With all of these attractive attributes, solid-state nanopores are a top contender for point-of-care diagnostic technologies. However, hindering their performance is an inability to distinguish between small molecules, pore-clogging, and the detection rate's dependence on sample concentration. The concentration-dependent detection rate becomes particularly evident at low sample concentrations (
Author: M. Muthukumar
Publisher: CRC Press
ISBN: 1420075179
Category : Science
Languages : en
Pages : 362
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Book Description
Polymer translocation occurs in many biological and biotechnological phenomena where electrically charged polymer molecules move through narrow spaces in crowded environments. Unraveling the rich phenomenology of polymer translocation requires a grasp of modern concepts of polymer physics and polyelectrolyte behavior. Polymer Translocation discusse
Author: Joshua Edel
Publisher: Royal Society of Chemistry
ISBN: 1849735271
Category : Technology & Engineering
Languages : en
Pages : 121
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Book Description
Bioanalytical applications in nanopores are an exciting field of research which have seen rapid development over the last ten plus years. This book focuses uniquely on this research area providing an insight from the world leading experts in the area. Based on the proceedings of the 1st International Conference on Nanopores for Bioanalytical Applications (Lanzarote, 2012), it will provide a snapshot of this exciting field for the nanopores community. It describes the latest experimental and fabrication protocols necessary to carry out nanopore-based experiments and covers a wide variety of topics. These include: biological pores, solid state pores, hybrid structures, fundamental aspects, work on the underlying (bio)physics and applications in biosensing and DNA sequencing. The multidisciplinary approach to this topic makes the book suitable for readers in both academia and industry.
Author: Ken Healy
Publisher:
ISBN:
Category : Microelectronics
Languages : en
Pages : 143
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Book Description
The main products of this work are a DNA analysis system based synthetic track-etched nanopores, and the results of experiments with this system detection of individual DNA molecules and discrimination between DNA fragment lengths. The majority of system components were developed from scratch, including a high-speed optical-feedback picoammeter which has potential for hundreds of kilohertz bandwidth, and a real-time data analysis system to detect and examine DNA translocation events immediately as they occur. Advancements in nanopore technology were also achieved, with improved fabrication and characterisation techniques. Also included in this thesis are comprehensive reviews of nanopore technologies and nanopore-based DNA analysis research, together with discussion of results achieved and a summary of future prospects. Nanopore-based analysis involves using an applied voltage to drive DNA molecules through a narrow pore which separates chambers of electrolyte solution. The applied voltage also drives a flow of electrolyte ions through the pore, measured as an electric current flowing through the electrodes used to apply it. When molecules pass through the pore they block the flow of ions, and thus their structure and length can be determined based on the degree and duration of the resulting current reductions. Track-etched nanaopores can be manufactured with sizes close to the diameter of DNA molecules, as necessary for nanopore-based DNA molecules, as necessary for nanopore-based DNA analysis. Their production involves irradiating thin polymer foils with single heavy ions, giving and individual nanometre-diameter linear track through each foil. These tracks have reduced density and disturbed molecular structure, meaning they will be preferentially attacked by chemical etchants, and so etching these foils creates nanopores. DNA molecules pass rapidly through nanopores, and the current levels involved are minuscule, requiring a sensitive and high-speed ammeter to detect them. Optical feedback is a modification to the traditional resistive-feedback approach for current measurement, where the feedback resistor is replaced by a LED photodiode pair. This eliminates bandwidth limitations associated with the resistor, thus giving the potential for higher-speed operation. Optical feedback also brings different noise characteristics, which are superior in certain situations where the current to be measured is low. Finally, all of the developments presented in thesis also form a platform for future research, which will further explore nanopore technologies and nanopore-based analysis of DNA and other biomolecules.
