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Author: Haralambos Stamatis
Publisher:
ISBN: 9781071622698
Category : Multienzyme complexes
Languages : en
Pages : 379
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Book Description
This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
Author: Haralambos Stamatis
Publisher:
ISBN: 9781071622698
Category : Multienzyme complexes
Languages : en
Pages : 379
Get Book Here
Book Description
This volume details cutting-edge methods and protocols for the development, characterization, and applications of multienzyme assemblies. Chapters guide readers through up-to-date techniques applied for the development and emerging applications of multi-enzymatic systems for biotransformations, biosensing, molecular-scale diagnostics and bioelectronics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Multienzymatic Assemblies: Methods and Protocols aims to be useful for academics and industry professionals working in the field of biotechnology, biochemistry, chemical and biological engineering, nanobiotechnology, and biocatalysis.
Author: Malcolm Dixon
Publisher: CUP Archive
ISBN:
Category : Enzymes
Languages : en
Pages : 120
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Book Description
Author: G. Rickey Welch
Publisher: Elsevier
ISBN: 0323149103
Category : Science
Languages : en
Pages : 473
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Book Description
Organized Multienzyme Systems: Catalytic Properties describes the kinetic and catalytic properties of organized enzyme systems. This book is composed of nine chapters that specifically cover both immobilized and naturally occurring systems. The first two chapters examine the nature and function of enzyme organization in the mitochondrion, as well as the structural/functional coupling of the components in energy-transducing membrane systems. These topics are followed by discussions on ""dynamic compartmentation"" in soluble multienzyme systems; the allosteric enzyme systems; and allosterism in reversibly adsorptive enzyme systems. Other chapters explore model studies with specific immobilized multienzyme sequences, as regards the analysis of microenvironmental effects, and the mathematical exposition on the kinetic analysis of multienzyme systems in homogeneous solution. The last chapters present some theoretical and experimental studies on the behavior of immobilized systems. These chapters also provide a speculative integrative view of the kind of functional coherence that may be operative in organized states in vivo. This book is of great value to cell biologists, biochemists, and enzyme scientists and researchers.
Author:
Publisher: ScholarlyEditions
ISBN: 1464945977
Category : Medical
Languages : en
Pages : 32
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Book Description
Multienzyme Complexes: Advances in Research and Application: 2011 Edition is a ScholarlyPaper™ that delivers timely, authoritative, and intensively focused information about Multienzyme Complexes in a compact format. The editors have built Multienzyme Complexes: Advances in Research and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Multienzyme Complexes in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Multienzyme Complexes: Advances in Research and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author:
Publisher: Academic Press
ISBN: 0128170751
Category : Science
Languages : en
Pages : 511
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Book Description
Metabolons and Supramolecular Enzyme Assemblies, Volume 617 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Dynamic plant metabolons, TCA cycle metabolons, the Chemotactic assembly of metabolons, Repurposing peroxisomes for metabolic engineering, Repurposing yeast mitochondria for metabolic engineering, Repurposing plant compartments for metabolic engineering, Protein scaffolds for pathway co-localization on lipid droplets, Engineered enzyme assemblies for metabolic engineering, NRPS assembly lines and P450 interactions, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in this series on enzymology Updated release includes the latest information on dynamic plant metabolons, TCA cycle metabolons, the chemotactic assembly of metabolons, and much more
Author: Steven E. Cohen (Ph. D.)
Publisher:
ISBN:
Category :
Languages : en
Pages : 148
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Book Description
The Wood-Ljungdahl pathway of acetogenesis allows for growth utilizing carbon dioxide as the sole carbon source. This process uses a series of metalloenzymes to catalyze the reduction of two molecules of carbon dioxide to acetyl-CoA. Key to this pathway are the bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase (CODH/ACS) and corrinoid iron-sulfur protein (CFeSP). CODH/ACS is a bifunctional enzyme, with the CODH subunit catalyzing the reduction of carbon dioxide to carbon monoxide. CFeSP is a cobalt-containing corrinoid-dependent methyltransferase. The active site of ACS, termed the A-cluster, is a nickel, iron, and sulfur-containing metallocluster which catalyzes the synthesis of acetyl-CoA from carbon monoxide provided by CODH, a methyl group provided by CFeSP, and CoA. Despite the unprecedented organometallic chemistry performed at the A-cluster much is unknown about this ACS activity. No substrate-bound structures of ACS have ever been reported and the molecular basis for ACS and CFeSP interactions are largely uncharacterized. Here we present the structure of the carbonylated A-cluster in CODH/ACS, highlighting the role of conformational dynamics in catalysis. We further characterize ACS dynamics using negative-stain electron microscopy, demonstrating a much larger extent of conformational flexibility than has been crystallographically observed. The basis for CFeSP:ACS interactions is further interrogated using a series of CFeSP domain constructs, showing the corrinoidbinding Rossmann fold to be sufficient for ACS interactions. Finally, we report preliminary structural characterization of acetyl-CoA decarbonylase/synthase (ACDS), a 2.2 MDa complex of CODH, ACS, and CFeSP found in archaeal methanogens. Together, this advances our understanding of the roles of structure and dynamics in the Wood-Ljungdahl pathway.
Author: Jacqueline D. Keighron
Publisher:
ISBN:
Category :
Languages : en
Pages : 182
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Book Description
Author: Robert H. Behal
Publisher:
ISBN:
Category :
Languages : en
Pages : 6
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Book Description
Author: Florian Xavier Delbart
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Author: David L. Bates
Publisher:
ISBN:
Category :
Languages : en
Pages : 4
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Book Description
