Molecular Genetic Analysis of SRF-6, a Gene Involved in Surface Antigen Switching in Caenorhabditis Elegans PDF Download
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Author: Brian Joseph Libby
Publisher:
ISBN:
Category : Caenorhabditis elegans
Languages : en
Pages : 310
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Book Description
Author: Brian Joseph Libby
Publisher:
ISBN:
Category : Caenorhabditis elegans
Languages : en
Pages : 310
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Book Description
Author: Joseph H. Chou
Publisher:
ISBN:
Category : Caenorhabditis elegans
Languages : en
Pages : 362
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Book Description
Author: Jörg Höflich
Publisher:
ISBN:
Category :
Languages : en
Pages : 148
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Book Description
Author: Frederick A. Partridge
Publisher:
ISBN:
Category : Caenorhabditis elegans
Languages : en
Pages : 0
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Author:
Publisher:
ISBN:
Category : Cytochemistry
Languages : en
Pages : 364
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ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 784
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Book Description
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 556
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Author:
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Category : Genetics
Languages : en
Pages : 506
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Book Description
Author:
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ISBN:
Category : Medicine
Languages : en
Pages : 1492
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Book Description
Author: Ian A. Hope
Publisher: OUP Oxford
ISBN: 019159198X
Category : Science
Languages : en
Pages : 306
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Book Description
Caenorhabditis Elegans has been a popular model organism for biological research for over thirty years and has been used to investigate many aspects of animal development, for example apoptosis, the Hox genes, signal transduction pathways, and the development of the nervous system. It has recently taken on new importance with the publication of the entire genome sequence in 1998. The first chapter gives all the basic information on C. elegans required to use it: it's natural history, anatomy, life cycle, development, and evolution. Information on how to obtain, grow, and maintain C. elegans for use as a model system is given in Chapter 4. Chapters 2 and 3 describe the genome project and show how to use genome sequence information by searching the database for homologues using different search methods and then how to analyse the search data. The next chapter gives the essential practical details of transformation and common uses for the technique. Chapter 6 covers reverse genetics and describes strategies for gene inactivation that are known to work in C elegans: epigenetic inactivation and mutational germ line inactivation. Chapter 7 is designed to help the user analyse phenotype by microscopy and includes Normaski, fluorescence, 4-dimensional, and electron microscopy. Techniques for studying the neurobiology of C. elegans are given in chapter 8. Chapter 9 describes the three commonly used approaches for studying gene expression and Chapter 10 deals with the common methods of molecular biology essential for gene characterization. C. elegans is not the ideal organism for biochemical studies, but chapter 11 describes several procedures for producing biochemically useful quantities of pure tissues. The final chapter is about conventional genetics and details the standard procedures for selfing and crossing; mutagenesis and mutant screening; characterization of mutants; gene mapping; temperature-shift experiments and mosaic analysis. Caenorhabditis Elegans: A Practical Approach will therefore provide all the background information necessary for use of C. elegans as a model system.
