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Author: Scott Orland Rogers
Publisher: CRC Press
ISBN: 1000596389
Category : Science
Languages : en
Pages : 376
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Book Description
DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership. Key Features Summarizes nucleic acid extractions from a wide variety of tissues and cells Describes processes of nucleic acid preservation Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses Includes additional methods useful for analysis of nucleic acids and proteins Related Titles DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7). Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9). Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9) Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)
Author: Scott Orland Rogers
Publisher: CRC Press
ISBN: 1000596389
Category : Science
Languages : en
Pages : 376
Get Book Here
Book Description
DNA and RNA extraction methods from a variety of tissues and samples are now routine, including extraction from single cells. Many methods are now automated. Sequencing efficiency has reached the point where it is now possible to obtain gigabases of data, both quickly and inexpensively. Such methods permit the identification of gene versions, including those associated with disease (e.g. small nucleotide polymorphism analyses, or SNPs). The general public as well as clinicians can now access a wide variety of literature on the molecular bases of diseases, allowing them to better assess disease risks and treatments. This volume concentrates on medically-focused methods, and therefore the major audience will be medical professionals, students, and those involved in medically-related research endeavors. There are also papers in this volume dealing specifically with methods developed to analyze large sequence data sets. Many methods reviewed herein are more broadly applicable to other fields in biology, chemistry, bioinformatics, and bioengineering, and are intended for a broad readership. Key Features Summarizes nucleic acid extractions from a wide variety of tissues and cells Describes processes of nucleic acid preservation Reviews forensic sampling, detection of nucleic acids, and delivery of nucleic acids to multicellular organisms Provides essential guidance for sequencing, sequence analysis, database searches, and phylogenetic analyses Includes additional methods useful for analysis of nucleic acids and proteins Related Titles DeSalle, et al. Phylogenomics: A Primer (ISBN 978-0-3670-2849-7). Jennings, W. B. Phylogenomic Data Acquisition: Principles and Practice (ISBN 978-0-3678-6980-9). Wang, X. Next-Generation Sequencing Data Analysis (ISBN 978-1-4822-1788-9) Sung, W.-K. Algorithms for Next-Generation Sequencing (ISBN 978-0-3676-5797-0)
Author: Andreas Hofmann
Publisher: Cambridge University Press
ISBN: 1107044707
Category : Business & Economics
Languages : en
Pages : 229
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Book Description
An accessible overview of the most popular and cutting-edge methods for studying the properties of molecules and their interactions.
Author: Xuhua Xia
Publisher: Springer Science & Business Media
ISBN: 030646893X
Category : Science
Languages : en
Pages : 284
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Book Description
Data Analysis in Molecular Biology and Evolution introduces biologists to DAMBE, a proprietary, user-friendly computer program for molecular data analysis. The unique combination of this book and software will allow biologists not only to understand the rationale behind a variety of computational tools in molecular biology and evolution, but also to gain instant access to these tools for use in their laboratories. Data Analysis in Molecular Biology and Evolution serves as an excellent resource for advanced level undergraduates or graduates as well as for professionals working in the field.
Author: Bruce Alberts
Publisher:
ISBN: 9780815332183
Category : Cytology
Languages : en
Pages : 0
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Book Description
Author:
Publisher:
ISBN: 9780471503385
Category : Molecular biology
Languages : en
Pages :
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Book Description
Author: Giorgio Stanta
Publisher: Springer Science & Business Media
ISBN: 3642178901
Category : Medical
Languages : en
Pages : 301
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Book Description
A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.
Author: Csaba Ortutay
Publisher: John Wiley & Sons
ISBN: 1119165024
Category : Medical
Languages : en
Pages : 354
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Book Description
This book addresses the difficulties experienced by wet lab researchers with the statistical analysis of molecular biology related data. The authors explain how to use R and Bioconductor for the analysis of experimental data in the field of molecular biology. The content is based upon two university courses for bioinformatics and experimental biology students (Biological Data Analysis with R and High-throughput Data Analysis with R). The material is divided into chapters based upon the experimental methods used in the laboratories. Key features include: • Broad appeal--the authors target their material to researchers in several levels, ensuring that the basics are always covered. • First book to explain how to use R and Bioconductor for the analysis of several types of experimental data in the field of molecular biology. • Focuses on R and Bioconductor, which are widely used for data analysis. One great benefit of R and Bioconductor is that there is a vast user community and very active discussion in place, in addition to the practice of sharing codes. Further, R is the platform for implementing new analysis approaches, therefore novel methods are available early for R users.
Author: Ralph Rapley
Publisher: John Wiley & Sons
ISBN: 0470020199
Category : Science
Languages : en
Pages : 391
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Book Description
This advanced level textbook provides a comprehensive overview ofrecent developments in the area of molecular based diagnostics(including nucleic acids, biosensors and immunoessays) of diseasemarkers. It also covers the impact of techniques such as in vitronucleic acid amplifications (e.g. PCR) and other amplificationmethods, as well as gene and biochip production and automatedtechniques such as fluorescent sequencing. The book discusses key concepts where new and merging areas,including pharmacogenomics, proteomics and functional genomics, arebeing researched and developed. In addition, examples are givenwhere this new area of bioscience has or may be successfullyapplied.
Author: Robert T. Gerlai
Publisher: Academic Press
ISBN: 0128041161
Category : Mathematics
Languages : en
Pages : 710
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Book Description
Molecular-Genetic and Statistical Techniques for Behavioral and Neural Research presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior, a critical piece of the puzzle for clinicians, scientists, course instructors and advanced undergraduate and graduate students. Chapters examine neuroinformatics, genetic and neurobehavioral databases and data mining, also providing an analysis of natural genetic variation and principles and applications of forward (mutagenesis) and reverse genetics (gene targeting). In addition, the book discusses gene expression and its role in brain function and behavior, along with ethical issues in the use of animals in genetics testing. Written and edited by leading international experts, this book provides a clear presentation of the frontiers of basic research as well as translationally relevant techniques that are used by neurobehavioral geneticists. Focuses on new techniques, including electrocorticography, functional mapping, stereo EEG, motor evoked potentials, optical coherence tomography, magnetoencephalography, laser evoked potentials, transmagnetic stimulation, and motor evoked potentials Presents the most exciting molecular and recombinant DNA techniques used in the analysis of brain function and behavior Written and edited by leading international experts
Author: Jacqueline Boultwood
Publisher: Springer Science & Business Media
ISBN: 1592591353
Category : Medical
Languages : en
Pages : 302
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Book Description
Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chapters describing techniques for the identification of chromosomal abnormalities and comprising: fluor- cent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), and microsatellite analysis. FISH has a pro- nent role in the molecular analysis of cancer and can be used for the detection of numerical and structural chromosomal abnormalities. The recently described SKY, in which all human metaphase chromosomes are visualized in specific colors, allows for the definition of all chromosomal rearrangements and marker chromosomes in a tumor cell. Protocols for the detection of chromosomal re- rangements by PCR and RT-PCR are described, as well as the technique of DNA fingerprinting, a powerful tool for studying somatic genetic alterations in tumorigenesis.
