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Author: P. Kucera
Publisher: Elsevier
ISBN: 0080858317
Category : Science
Languages : en
Pages : 319
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Book Description
Microcolumn High-Performance Liquid Chromatography
Author: P. Kucera
Publisher: Elsevier
ISBN: 0080858317
Category : Science
Languages : en
Pages : 319
Get Book Here
Book Description
Microcolumn High-Performance Liquid Chromatography
Author: Kucera P.
Publisher:
ISBN: 9780444416162
Category :
Languages : en
Pages : 302
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Book Description
Author: J. F. Parcher
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 272
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Book Description
Here, authors specializing in different branches of chromatography--including gas chromatography, supercritical fluid chromatography, and high-pressure liquid chromatography--describe their fields while drawing out connections with other branches.
Author: Toshihiko Hanai
Publisher: World Scientific
ISBN: 9789810219062
Category : Science
Languages : en
Pages : 366
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Book Description
This book is a contemporary review of selected subjects in liquid chromatography, especially of the technical development, rather than the applications. The subjects are focused in the biomedical and environmental fields. This is also a troubleshooting record. Complex analytical problems such as sensitivity (sensitive detection by chemiluminescence, coulometric detection, laser based detection, necessity of degassing the system for sensitive detection), difficulty (free radical detection by Electron Spin Resonance, Polarimeter for chiral recognition) and reproducibility (packings for chiral separation and stable bonded silica gels) are solved. Theoretically and environmentally important miniaturizations are described. Individual chapters written by specialists provide information beyond what can be found in general textbooks of liquid chromatography.
Author: Colin T. Mant
Publisher: CRC Press
ISBN: 1351440748
Category : Medical
Languages : en
Pages : 887
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Book Description
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Author: Vicki Lynn McGuffin
Publisher:
ISBN:
Category : Liquid chromatography
Languages : en
Pages : 670
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Book Description
Author: Milos Krejci
Publisher: CRC Press
ISBN: 1000104761
Category : Science
Languages : en
Pages : 217
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Book Description
This book provides a detailed description of technical elements of a microbore column liquid chromatograph suitable for use in trace analysis. It presents examples of analyses, especially from the spheres of biochemistry, pharmacology, and environmental analytical chemistry.
Author: K. Zech
Publisher: Elsevier
ISBN: 0080858430
Category : Science
Languages : en
Pages : 407
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Book Description
This is the second of a two-volume project which treats the handling, separation and detection of complex samples as an integrated, interconnected process. On the basis of this philosophy the editors have selected those contributions which demonstrate that optimal sample preparation leads to a simplification of detection or reduced demands on the separation process. Throughout the book emphasis is on chemical principles with minimum discussion of the equipment required - an approach which reflects the editors' view that the limiting factor in the analysis of complex samples is an incomplete knowledge of the underlying chemistry rather than the hardware available. This lack of knowledge becomes more evident as the demands for lower detection limits grow, as solving complex matrix problems requires a greater understanding of the chemical interaction between the substance to be analysed and the stationary phase. Thus, apart from one chapter dealing with chemically modified silicas, the main theme of the book is developed in three chapters on sample preparation and three on detection.The opening chapter outlines concentration and chromatography on chemically modified silicas with complexing properties, and gives examples of the use of these phases with organic and inorganic compounds. Chapter II, the first of the three contributions dealing with sample preparation, addresses such questions as whether the prepared sample is representative of the material to be analysed; how to avoid contamination; which separation procedure should be used to avoid tedious sample preparation. Chapter III describes the processing of whole blood for drug analysis. The determination of cyclosporine and its metabolites (an especially difficult case) demonstrates how comprehensive the optimisation of sample preparation must be to successfully perform the analysis. Several other examples are also given. Chapter IV deals with radio-column liquid chromatography and introduces the other theme of the book, i.e. selective detection methods. The widespread use of radioisotopes requires a high degree of purification during the manufacture of the compounds, as well as highly accurate detection methods in biological and biochemical studies.Chapter V continues the theme of selective detection with an overview of post-column reaction detection. The use of immobilised enzymes in post-column reactors or `pumpless' reactor systems for on-line reagent generation after the chromatographic separation step is discussed in detail. Various examples of the separation of biological compounds show how the production of electrochemical reagents and photochemical reaction detection have increased the selectivity of the detection, leading to more economical analytical systems. Selective detection employing luminescence detection techniques is outlined in Chapter VI. The use of immobilised fluorophores or the coupling to photochemical reactions leads to highly selective detection systems which can greatly simplify the sample handling. The final chapter reviews the use of continuous separation techniques in flow injection analysis thus revealing the need for a strong interdisciplinary dependence between sample handling and separation in this area.Written by experienced practitioners, this book will be extremely useful to investigators in many areas of application. Each chapter includes sufficient references to the literature to serve as a valuable starting point for more detailed investigation. The strong emphasis on sample handling makes the book unique in many ways and it will be welcomed by environmental scientists as well as those active in the clinical, pharmaceutical and bioanalytical fields.
Author: M.V. Novotny
Publisher: Elsevier
ISBN: 0080858333
Category : Science
Languages : en
Pages : 349
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Book Description
Edited by two of the pioneers of microcolumn chromatography and written by recognized experts in the field, this book summarizes advances in microcolumn liquid chromatography, capillary supercritical fluid chromatography and microelectrophoresis. Its unique combination of expert knowledge from leading laboratories in the USA, Japan and Switzerland, results in a particularly in-depth and comprehensive coverage of the various aspects of microcolumn separation methods.
Author: Cheryl Lynn Flurer
Publisher:
ISBN:
Category :
Languages : en
Pages : 736
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Book Description
