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Author: Hans-UIrich Bergmeyer
Publisher: Elsevier
ISBN: 0323161375
Category : Science
Languages : en
Pages : 640
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Book Description
Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase. This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.
Author: Hans-UIrich Bergmeyer
Publisher: Elsevier
ISBN: 0323161375
Category : Science
Languages : en
Pages : 640
Get Book Here
Book Description
Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltransferase and transamidinase to transketolase, transaldolase, UDP-glucuronyltransferase, glutamate-pyruvate transaminase, and phosphotransferases. Part III discusses hydrolases including esterases, glycoside hydrolases, peptidases, and proteinases, whereas Part IV looks at lyases, isomerases, and ligases, such as fructose-1, 6-diphosphate aldolase, 1-phosphofructoaldolase, glucosephosphate isomerase, and tetrahydrofolate formylase. This book is a valuable resource for biochemists as well as students and researchers working in the field of analytical biochemistry.
Author: Hans-UIrich Bergmeyer
Publisher: Elsevier
ISBN: 0323141773
Category : Science
Languages : en
Pages : 1093
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Book Description
Methods of Enzymatic Analysis focuses on the general progress in enzymology and in the special field of enzymatic analysis. This book explores the commercial production of biochemical reagents for analysis and explains the transition from the possible use of enzymatic analysis to its various applications in pure and applied biochemistry. Organized into four sections, this book starts with an overview of the basis of enzymatic analysis and provides general experimental guidelines for the techniques of measurement and for the disintegration of cells and tissues. This text then provides detailed instructions for the determination of substrates and assay of enzyme activities. Other chapters explore the practical aspects and information necessary for the application of reagents to enzymatic analysis, including sources, stability, and purity required. The final section describes the commercially available enzymes, coenzymes, substrates, and several less common reagents. Biochemists, biophysicists, researchers, and graduate students will find this book extremely useful.
Author: Hans-UIrich Bergmeyer
Publisher: Elsevier
ISBN: 0323161324
Category : Science
Languages : en
Pages : 761
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Book Description
Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid metabolites, lipids, and steroids ranging from polyunsaturated fatty acids and lecithin to choline, acetylcholine, triglycerides, glycerol, acetoacetate, triacetate, fumarylacetoacetate, 20-ketosteroids, prostaglandins, bile acids, and cholesterol. Part III discusses nucleic acids, purines, pyrimidines, nucleosides, coenzymes, and related compounds, whereas Part IV looks at other substrates and effectors such as inorganic phosphate. The book concludes with a chapter on metabolites and their concentrations in animal tissues. Biochemists as well as students and researchers working in the field of analytical biochemistry will find this book highly informative.
Author: Hans-Ulrich Bergmeyer
Publisher: Wiley-Blackwell
ISBN: 9783527260430
Category : Science
Languages : en
Pages : 606
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Book Description
Author: Minor Coon
Publisher: Elsevier
ISBN: 0323143873
Category : Medical
Languages : en
Pages : 655
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Book Description
Microsomes, Drug Oxidations, and Chemical Carcinogenesis, Volume II, documents the proceedings of the 4th International Symposium on Microsomes and Drug Oxidations held in Ann Arbor, July 1979. The symposium reviewed progress in the understanding of scientific and biomedical problems from a biochemical, biophysical, pharmacological, and toxicological perspective. Volume I contained 117 contributions made by researchers at the symposium, which were organized into three sections (Sections I-III). This second volume contains 122 contributions, divided into four sections (Sections IV-VII). The papers on Section IV examine the metabolic fate of oxygenated compounds. Section V provides studies on microsomal enzymes and lipid metabolism. Section VI includes papers on microsomal enzymes and toxicity of foreign compounds. Section VII covers microsomal enzymes and chemical mutagenesis and carcinogenesis. This book seeks to aid future progress in understanding the complexities of metabolic transformations by these versatile enzyme systems that act on physiologically important lipids as well as on a wide array of foreign substances, including drugs, anesthetics, industrial chemicals, food additives, pesticides, carcinogens, and nonnutrient dietary chemicals.
Author: Małgorzata Pawłowska
Publisher: Routledge
ISBN: 1000398781
Category : Technology & Engineering
Languages : en
Pages : 314
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Book Description
A side-effect of numerous anthropogenic activities involves unfavourable changes in the natural environment. The acquisition of natural resources, especially fossil fuels, solid waste and wastewater production, as well as emission of gases and particulate matter from industrial plants and means of transport contribute to disturbances in the natural cycles of elements between different parts of the environment. Local changes lead to global effects, changing the composition of atmosphere, its capacity for absorbing the infrared radiation and temperature, which has further repercussions in the form of weather anomalies, melting glaciers, flooding, migration or extinction of species, social problems, etc. These global changes can be mitigated by local remedial actions, simultaneously taken all over the world, including Poland. Only the joint efforts of communities from different countries can be successful in preserving the world as we know it for the future generations. Realisation of this task requires the cooperation of experts across many fields of science, environmental engineering being one of most relevant. It comprises the engineering actions taken to preserve the balance of the natural environment or restore it if degradation has occurred. This monograph presents several key issues related to the actions aimed at mitigating the negative impact on the environment connected with the acquisition and transport of energy, management of municipal and industrial wastes, as well as the impact of the industry on the aquatic and soil environment. This book is dedicated to academics, engineers, and students involved in environmental engineering, who are following the advances in the research on environmental aspects of energy production and waste management.
Author: George Charalambous
Publisher: Elsevier
ISBN: 0323155243
Category : Science
Languages : en
Pages : 340
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Book Description
Liquid Chromatographic Analysis of Food and Beverages, Volume 2 focuses on the role and utilization of reversed-phase separation techniques in the food, agricultural, biochemical, biomedical, and clinical area. This book discusses the high pressure liquid chromatography; estimation of dextrose equivalent value of starch hydrolysates from liquid chromatographic profiles; and analysis of gluconic acid in botrytized wines. The HPLC of carbohydrate products; reducing sugar derivatization for ultraviolet absorption detection in HPLC analyses; and quantitative determination of dextromethorphan hydrobromide in cough remedies by high precision liquid chromatography are also elaborated. This text likewise discusses the separation of hop compounds by reverse-phase HPLC and analysis of polymethoxylated flavones in orange juice and fruit parts. This book is a good reference for food technologists and researchers conducting work on liquid chromatographic analysis of food and beverages.
Author: Karlfried Gawehn
Publisher: Academic Press
ISBN:
Category : Science
Languages : en
Pages : 772
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Book Description
Methods of Enzymatic analysis V4 ...
Author: Robert Eisenthal
Publisher: Practical Approach
ISBN: 9780199638208
Category : Analysis
Languages : en
Pages : 310
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Book Description
Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.
Author: F. Vardar-Sukan
Publisher: Springer Science & Business Media
ISBN: 940112468X
Category : Science
Languages : en
Pages : 554
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Book Description
In last decades rapid scientific and engineering developments have been occuring within the context of Biotechnology. If the World Economy is to benefit fully from the advances in biosciences and biochemical engineering, it must be able to focus new knowledge on commercially appropriate targets. Modern Biotechnology is a mixture of far reaching innovation superimposed on an industrial background and it represents a means of production with bright prospects, challenging problems and stimulating competition. This NATO Advanced Study Institute on "RECENT ADVANCES IN INDUSTRIAL APPLICATIONS OF BIOTECHNOLOGY" held between September 16-27, 1991 in Ku§Etdasl was the first ASI on Biotechnology :Ln Turkey. !t was aiming to provide an updated overview of the fundamental principles, novel application areas and impact of Biotechnology on international economy. Recent developments in the field of Biotechnology have been thoroughly discussed, concentrating on various interdisciplinary aspects. The illain lectures presented at the Institute covered both scientific and commercial aspects of new developments in biotechnology and discussed the possible ways of meeting the challenges of the industry. The main lectures were supplemented by Oral 2nd Poster Presentations. Thus, this volume is comprised of three sections. Part I contains the i~vited lectures and Part II oral presentations. Exte~ded abstracts of poster presentations have been included in Part III to provide a more comprehensive coverage of the ASI.
