Methods in Cell Wall Cytochemistry PDF Download
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Author: K V Krishnamurthy
Publisher: CRC Press
ISBN: 1000144151
Category : Science
Languages : en
Pages : 344
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Book Description
Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
Author: K V Krishnamurthy
Publisher: CRC Press
ISBN: 1000144151
Category : Science
Languages : en
Pages : 344
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Book Description
Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
Author: Farr Cytochemical Laboratories
Publisher:
ISBN:
Category : Cytochemistry
Languages : en
Pages : 84
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Book Description
As a part of the research program designed to solve the nutritional problems of astronauts during extended explorations of space, cell wall substances of four fungi, Heterocephalum aurantiacum, Spicaria sp., Cladosporium sp. and Linderina pennispora have been identified and localized by standard cytochemical methods. Chitin is the resistant cell wall material in the component cells of all of these fungi and results of efforts to identify cellulose in the same cell walls have been negative. Excretions which accumulate on the surfaces of the aerial corticating hyphae of H. aurantiacum are composed primarily of calcium salts and an unidentified colloidal material, yellow-orange in color and having many characteristics in common with the so-called fungal gamboge. Reactions of the previously untreated chitinous cell walls to the enzyme, chitinase, are positive. Enzymatic hydrolysis represents the most suitable method now available for the conversion of chitin to N-acetylglucosamine with the accompanying breakdown of the cell wall and concurrent release of nutritive subbussubstances i ses in the protoplast. X-rray diffraction patteettterns of chitin havevah nin have been obtained from purified cell wall samples. Measurements of these same samples, at successive stages ofoof removal of non-chitinous materials, show a gradual increase in refractive index values. (Author).
Author: Wanda K. Farr
Publisher:
ISBN:
Category : Astronauts
Languages : en
Pages : 84
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Book Description
As a part of the research program designed to solve the nutritional problems of astronauts during extended explorations of space, cell wall substances of four fungi, Heterocephalum aurantiacum, Spicaria sp., Cladosporium sp. and Linderina pennispora have been identified and localized by standard cytochemical methods. Chitin is the resistant cell wall material in the component cells of all of these fungi and results of efforts to identify cellulose in the same cell walls have been negative. Excretions which accumulate on the surfaces of the aerial corticating hyphae of H. aurantiacum are composed primarily of calcium salts and an unidentified colloidal material, yellow-orange in color and having many characteristics in common with the so-called "fungal gamboge." Reactions of the previously untreated chitinous cell walls to the enzyme, chitinase, are positive. Enzymatic hydrolysis represents the most suitable method now available for the conversion of chitin to N-acetylglucosamine with the accompanying breakdown of the cell wall and concurrent release of nutritive substances in the protoplast. X-ray diffraction patterns of chitin have been obtained from purified cell wall samples. Measurements of these same samples, at successive stages of removal of non-chitinous materials, show a gradual increase in refractive index values. Progress has been made in the adaptation of interference microscopy to the study of chitinous cell walls during the process of hydrolysis with chitinase.
Author: Chris Hawes
Publisher: OUP Oxford
ISBN: 019156611X
Category : Science
Languages : en
Pages : 364
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Book Description
With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, including classical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulation and electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advanced students, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.
Author:
Publisher: Academic Press
ISBN: 0080858996
Category : Science
Languages : en
Pages : 445
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Book Description
Methods in Cell Biology
Author: Wanda K. Farr
Publisher:
ISBN:
Category :
Languages : en
Pages : 91
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Book Description
Cytochemical analyses of the four imperfect fungi, Cylindrocephalum sp., Curvularia lunata, Aspergillus oryzae, and Gliocladium deliquescens showed that the resistant material in their cell walls is chitin. All efforts to identify cellulose produced negative results. Solutions of chitinase-containing Worthington Lysozyme (Muramidase) in concentrations of 0.5mg./ml. brought about dissolution of the cell walls and dispersion of the cell contents. Enzymatic hydrolysis represents the most suitable method now available for the conversion of fungal cell wall chitin to digestible end products with the simultaneous release of nutrient substances in the protoplasm. Specific studies have continued on protoplasmic phenomena within the cells of fungi examined, especially Heterocephalum aurantiacum Plastid-like structures in the protoplasts are the cell organs which produce chitin. Chitin-forming plastids increase in number by direct division and the quantities of chitin at their surfaces, in a given cell, may be equal to or greater than the amount of chitin in the cell wall. This information necessitates the inclusion of chitin located in the protoplasts, as well as that in the cell walls, in any considerations of fungal tissues as food for astronauts in extended explorations of space. (Author).
Author: Kazuo Ogawa
Publisher: CRC Press
ISBN:
Category : Medical
Languages : en
Pages : 828
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Book Description
Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine provides up-to-date, comprehensive surveys and descriptions of electron microscopic cytochemical and immunocytochemical methods. It also reviews selected topics of cell biology, pathology, histology, and biomedicine. These reviews illustrate the fundamental contributions of electron microscopic cytochemistry and immunocytochemistry to biology and pathology. By bringing together methodology and the application of methods, this book presents a broad discussion of the contributions, potentials, and limitations of cytochemistry and immunocytochemistry. Electron Microscopic Cytochemistry and Immunocytochemistry in Biomedicine will be a useful reference volume for cell biologists, histologists, anatomists, pathologists, physiologists, immunologists, and clinical researchers concerned with the basic mechanisms of diseases.
Author: John Lloyd Hall
Publisher: Elsevier-North-Holland Biomedical Press
ISBN:
Category : Science
Languages : en
Pages : 464
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Book Description
Author: Wanda K. Farr
Publisher:
ISBN:
Category : Chitin
Languages : en
Pages : 84
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Book Description
As a part of the research program designed to solve the nutritional problems of astronauts during extended explorations of space, cell wall substances of four fungi, Heterocephalum aurantiacum, Spicaria sp., Cladosporium sp. and Linderina pennispora have been identified and localized by standard cytochemical methods. Chitin is the resistant cell wall material in the component cells of all of these fungi and results of efforts to identify cellulose in the same cell walls have been negative. Excretions which accumulate on the surfaces of the aerial corticating hyphae of H. aurantiacum are composed primarily of calcium salts and an unidentified colloidal material, yellow-orange in color and having many characteristics in common with the so-called "fungal gamboge." Reactions of the previously untreated chitinous cell walls to the enzyme, chitinase, are positive. Enzymatic hydrolysis represents the most suitable method now available for the conversion of chitin to N-acetylglucosamine with the accompanying breakdown of the cell wall and concurrent release of nutritive substances in the protoplast. X-ray diffraction patterns of chitin have been obtained from purified cell wall samples. Measurements of these same samples, at successive stages of removal of non-chitinous materials, show a gradual increase in refractive index values. Progress has been made in the adaptation of interference microscopy to the study of chitinous cell walls during the process of hydrolysis with chitinase.
Author: M.A. Hayat
Publisher: Springer Science & Business Media
ISBN: 9780306442940
Category : Science
Languages : en
Pages : 460
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Book Description
The author's primary aim is to thoroughly explain the biochemical concepts governing cytochemical procedures for transmission electron microscopy. Such information provides undergraduate and graduate students, technicians, and researchers with a more profound understanding of electron micrographs, as well as the knowledge to refine existing techniques and develop new methodologies.
