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Author: Bengt Nordén
Publisher: Royal Society of Chemistry
ISBN: 1788018214
Category : Science
Languages : en
Pages : 305
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Book Description
This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.
Author: Bengt Nordén
Publisher: Royal Society of Chemistry
ISBN: 1788018214
Category : Science
Languages : en
Pages : 305
Get Book Here
Book Description
This book provides an introduction to optical anisotropy (linear dichroism, LD) and optical activity (circular dichroism, CD) as techniques for the study of structures and interactions of molecules in solution. The book covers the use of these techniques for both small and large molecular systems with particular emphasis being placed on proteins and nucleic acids. CD is a well-established technique and this book aims to explain how it can be used simply and effectively for new entrants to the field as well as covering more advanced techniques for experts. LD is often seen as a rather exotic method intended only for experienced spectroscopists. This book demonstrates that it is an approach with real utility that may be used by both students and scientists from graduate level onwards to give simple answers, which are not available from any other technique, to structural and kinetic questions. Much of the emphasis is on flow orientation of samples in solution phase. The book first describes the techniques and the information they can provide; it then goes on to give specific details on how to actually implement them, including a wide range of examples showing how LD and CD can help with * protein and nucleic acid secondary structure elucidation; * analysis of the formation and rearrangements of fibrous proteins and membrane proteins; * identification of the absolute configuration of small molecules; * determination of the orientation of small molecules in anisotropic media; * assignment of transition moment polarizations; * investigation of binding strengths and geometries of ligand-macromolecule complexes; * 3-D structure determination from LD, molecular replacement and MD modeling. The advantages of combined LD/CD studies are also outlined with examples of DNA/drug complexes and protein insertion into membranes. Taken together the book represents a comprehensive text on the theory and application of LD and CD in the chemical and biological sciences.
Author: Alison Rodger
Publisher: Oxford University Press, USA
ISBN: 9780198558972
Category : Science
Languages : en
Pages : 170
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Book Description
This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.
Author: W. Roy Mason
Publisher: John Wiley & Sons
ISBN: 0470139226
Category : Science
Languages : en
Pages : 237
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Book Description
The book is a technical guide for chemists and spectroscopists, and presents a concise description of magnetic circular dichroism (MCD) spectroscopy and how it has advanced the interpretation of molecular electronic spectra. Provides a practical guide to utilizing MCD spectroscopy for chemists starting in the field Written by an expert with over twenty years of experience in the field Helps the reader to visualize the optical spectroscopic effects presented by MCD measurements Includes practical considerations for experimental MCD measurements based on the author's experience Written as a general discussion of the subject matter, with illustrative examples provided and discussed in the case studies to show the breadth of application of MCD measurements.
Author: Bonnie Ann Wallace
Publisher: IOS Press
ISBN: 1607500000
Category : Medical
Languages : en
Pages : 244
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Book Description
Presents an account of circular dichroism (CD) spectroscopy and its application to structural biology. This book covers the methods of synchrotron radiation circular dichroism (SRCD) and linear dichroism (LD).
Author: Bengt Nordén
Publisher: Lund University Press
ISBN:
Category : Science
Languages : en
Pages : 278
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Book Description
Author: J.L. Koenig
Publisher: Elsevier
ISBN: 0080541577
Category : Technology & Engineering
Languages : en
Pages : 509
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Book Description
This revised and updated Second Edition of the best-selling reference/text is essential reading for students and scientists who seek a thorough and practical introduction to the field of polymer spectroscopy. Eleven chapters cover the fundamental aspects and experimental applications of the primary spectroscopic methods. The advantages and disadvantages of the various techniques for particular polymer systems are also discussed. The goal of the author is not to make the reader an expert in the field, but rather to provide enough information about the different spectroscopic methods that the reader can determine how the available techniques can be used to solve a particular polymer problem. This Second Edition contains new and updated information on techniques in IR and NMR, as well as an all-new chapter on Mass Spectrometry.
Author: Nagao Kobayashi
Publisher: Royal Society of Chemistry
ISBN: 1849732930
Category : Science
Languages : en
Pages : 217
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Book Description
CD and MCD spectroscopy can provide key information about the conformations and electronic states of chromophore containing molecules. However, the theory has remained too challenging and inaccessible for many organic chemists and biochemists and only a few researchers have carried out detailed quantitative analyses of their spectral data. This is not surprising as people who excel at spectroscopic theory usually lack the skills set required to design and synthesise the molecules that would be most appropriate for describing and explaining the theory of CD and MCD spectroscopy. Most of the books that have been written on the subject have, therefore, been based on very dense sets of mathematical equations. This timely book rectifies that situation by summarizing the relationship between the different types of spectra and by describing in detail the qualitative and quantitative methods which can readily be used to analyse CD and MCD spectral data. During the last decade the authors have successfully synthesized several molecules to illustrate key points related to the theory of CD and MCD spectroscopy, resulting in this definitive book providing key practical knowledge in a readily accessible style. It is aimed primarily at organic chemists and biochemists and is required reading for researchers active in the field. In the introduction, the book describes the types of information that can be derived from CD and MCD spectroscopy. After a detailed explanation of the theory of electronic absorption spectroscopy, it then provides practical in depth examples of the various analytical methods that can be carried out with CD and MCD spectral data. This makes the theory of these techniques much more accessible for researchers who do not specialise in physical chemistry. The book concludes by briefly summarizing the key mathematical expressions associated with each technique.
Author: Jeroen A. van Bokhoven
Publisher: John Wiley & Sons
ISBN: 1118844238
Category : Science
Languages : en
Pages : 940
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Book Description
X-Ray Absorption and X-ray Emission Spectroscopy: Theory and Applications During the last two decades, remarkable and often spectacular progress has been made in the methodological and instrumental aspects of x-ray absorption and emission spectroscopy. This progress includes considerable technological improvements in the design and production of detectors especially with the development and expansion of large-scale synchrotron reactors All this has resulted in improved analytical performance and new applications, as well as in the perspective of a dramatic enhancement in the potential of x-ray based analysis techniques for the near future. This comprehensive two-volume treatise features articles that explain the phenomena and describe examples of X-ray absorption and emission applications in several fields, including chemistry, biochemistry, catalysis, amorphous and liquid systems, synchrotron radiation, and surface phenomena. Contributors explain the underlying theory, how to set up X-ray absorption experiments, and how to analyze the details of the resulting spectra. X-Ray Absorption and X-ray Emission Spectroscopy: Theory and Applications: Combines the theory, instrumentation and applications of x-ray absorption and emission spectroscopies which offer unique diagnostics to study almost any object in the Universe. Is the go-to reference book in the subject for all researchers across multi-disciplines since intense beams from modern sources have revolutionized x-ray science in recent years Is relevant to students, postdocurates and researchers working on x-rays and related synchrotron sources and applications in materials, physics, medicine, environment/geology, and biomedical materials
Author: James A. Samson
Publisher: Academic Press
ISBN: 0080543480
Category : Science
Languages : en
Pages : 317
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Book Description
Techniques of Vacuum Ultraviolet Spectroscopy was first published in 1967. In the three decades since, the techniques associated with vacuum ultraviolet spectroscopy have been greatly expanded. Originally published as two volumes in the serial "Experimental Methods in the Physical Sciences," Vacuum Ultraviolet Spectroscopy combines in one paperback volume information on the many advances in vacuum ultraviolet (VUV) research. In addition, the book provides students and researchers with concise reviews of the important aspects of designing experiments in the VUV region.This is the only comprehensive treatise describing the use of synchrotron and other light sources for research, along with the new technologies in optical elements, multilayers, mirror coatings, soft x-ray zone plates, VUV detectors, interferometric spectrometers, and subjects such as spectromicroscopy, lithography, and photon-induced fluorescence. Vacuum Ultraviolet Spectroscopy is an ideal handbook both for the beginner and for the experienced researcher in any field requiring the use of VUV radiation. Key Features* Detailed review of synchrotron radiation sources including undulators and wigglers* Comprehensive outline of monochromator design* Concise review of optics theory for multilayers, spectrometers, and zone plates* Information about other important VUV sources such as laser produced plasmas and Electron Beam Ion Trap (EBIT) sources* Applications such as spectromicroscopy, lithography, and fluorescence
Author: Prasad L. Polavarapu
Publisher: CRC Press
ISBN: 1315357194
Category : Science
Languages : en
Pages : 298
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Book Description
This book details chiroptical spectroscopic methods: electronic circular dichroism (ECD), optical rotatory dispersion (ORD), vibrational circular dichroism (VCD), and vibrational Raman optical activity (VROA). For each technique, the text presents experimental methods for measurements and theoretical methods for analyzing the experimental data. It also includes a set of experiments that can be adopted for undergraduate teaching laboratories. Each chapter is written in an easy-to-follow format for novice readers, with necessary theoretical formalism in appendices for advanced readers.
