Laser Induced Fluorescence Line Narrowing Studies of Praseodymium in Lanthanum Trifluoride and Application of Pressure Scanning to the Tuning of a High Resolution Dye Laser PDF Download
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Author: Robert Flach
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Category : Dye lasers
Languages : en
Pages : 264
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Author: Robert Flach
Publisher:
ISBN:
Category : Dye lasers
Languages : en
Pages : 264
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Category : Aeronautics
Languages : en
Pages : 268
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Category : Dissertations, Academic
Languages : en
Pages : 756
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Category : Dissertations, Academic
Languages : en
Pages : 924
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Category : Union catalogs
Languages : en
Pages : 1036
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Author: Laura Tannas Skiff
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Category :
Languages : en
Pages : 194
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Category : Dissertations, Academic
Languages : en
Pages : 1008
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Author: Chen Lin
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Category :
Languages : en
Pages :
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Low-temperature laser-induced fluorescence spectroscopy has various applications in analytical, physical, and biophysical chemistry. This technique provides information on the fluorescence origin band, zero-phonon lines and phonon-sidebands, inhomogeneous broadening, electron-phonon coupling strength, and ground- and excited-state vibrational frequencies of studied molecules. Examples discussed in this work include studies of DNA/metabolites and monoclonal antibody (mAb)/antigen interactions. The structural basis for the increased reactivity of BPDE towards guanines at 5-methylcytosine ([superscript]M[superscripte]eC):G sites in DNA was investigated by low temperature laser-based spectroscopy, studying the nature of physical complexes of benzo[a]pyrene tetraol in a series of 5-methylcytosine structural DNA analogs. We found that the presence of a C-5 substituent on cytosine and related structural modifications influences the conformation of BPT in DNA analogs, and could explain the increase in guanine reactivity at [superscript]M[superscript]eC:G sites of the p53 tumor suppressor gene that contains endogenenous 5-([superscript]M[superscript]eC. It has been demonstrated that various mAbs can bind a particular cross-reactant by adopting two distinct "red" and "blue" conformations of its binding sites. We showed that the blue conformation of pyrene in several mAbs (including 4D5 mAb) is consistent with [pi]-cation interactions, underscoring the importance of [pi]-cation interaction in ligand binding. We propose that considerable narrowing of the fluorescence origin band of the ligand in the protein environment could be regarded as a simple indicator of [pi]-cation interactions. It is also shown that time-resolved delta fluorescence line-narrowing ([delta]FLN) spectroscopy, using excitation within the (0,0)-transition band, provides more reliable information of the frequency dependence of the electron-phonon coupling (Huang-Rhys factor, (S
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Languages : en
Pages :
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Laser-induced fluorescence line narrowing reveals structural changes in Eu/sup 3 +/ doped borate glasses as a function of Li2O network modifier concentration. These changes are discussed in terms of different coordination and bonding and possible phase separation.
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Languages : en
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Paramagnetic ions in glass reside in a distribution of physically different environments. By using a tunable laser source, selected groups of ions within an inhomogeneously broadened absorption band are excited and line-narrowed fluorescence is observed. The technique of laser-induced fluorescence line narrowing has been applied to several rare-earth ions in glasses to investigate site-to-site variations in energy levels, probabilities for radiative and non-radiative transitions, homogeneous line broadening, and energy transfer between ions. Results and interpretations of these experiments are reviewed, and possible extensions of these techniques are discussed. 25 references, 3 fig.
