Isolation and Characterization of a Genetic Locus Involved in Growth and Pathogenicity on Tomato Plants by Pseudomonas Syringae Pv. Tomato PDF Download
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Author: Dennis P. Jackson
Publisher:
ISBN:
Category : Pseudomonas syringae
Languages : en
Pages : 340
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Author: Dennis P. Jackson
Publisher:
ISBN:
Category : Pseudomonas syringae
Languages : en
Pages : 340
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: K. Rudolph
Publisher: Springer Science & Business Media
ISBN: 9780792346012
Category : Science
Languages : en
Pages : 714
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Book Description
During the last decade, research on Pseudomonas syringae pathovars and related pathogens has progressed rapidly, opening up many new avenues. The application of molecular genetics has provided new insights into determinants of pathogenicity and virulence. Progress has also been made in elucidating the chemical structures and modes of action of phytotoxins from Pseudomonas syringae; by establishing novel strategies for disease control; in biotechnological applications; by studying the resistant reaction of the plant with a combined biochemical and genetic approach; and in the development of new detection and identification methodologies as tools in epidemiological studies. With such rapid advances it becomes more and more difficult to keep abreast of the developments and concepts within disciplines, all involving research on pathovars of P. syringae. In an attempt to provide a balanced overview, recent developments in these rapidly expanding fields have been critically reviewed at the beginning of each chapter by internationally renowned experts. Our comprehensive coverage has been made possible because all the contributors to this volume presented their latest findings at the `5th International Conference on Pseudomonas syringae Pathovars and Related Pathogens' in Berlin, September 3-8, 1995. In this way, it was possible to bring together contributions from a wide range of fields including phytopathology, genetics, bacteriology, plant breeding, plant protection, and taxonomy. This book is not intended simply as a record of the proceedings of the Berlin Conference, but as an extension of recent findings and hypotheses put forward at the meeting. All papers published in this volume have been reviewed by the Editors.
Author: Nikolaos Skandalis
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Jennifer Mae Lorang
Publisher:
ISBN:
Category : Bacterial diseases of plants
Languages : en
Pages : 196
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Author: Rhonda Lynn Schwartz
Publisher:
ISBN:
Category :
Languages : en
Pages : 172
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Author: Wayne Lee Wiebe
Publisher:
ISBN:
Category :
Languages : en
Pages : 122
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Author: Yuqi Zhao
Publisher:
ISBN:
Category : Molecular genetics
Languages : en
Pages : 434
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A pathogenicity locus of Pseudomonas syringae pv. syringae identified by Tn5 mutagenesis was investigated. The mutant strain PS9024 is attenuated for disease expression in its host, Phaseolus vulgaris, but produces the hypersensitive reaction (HR) in the nonhost, tobacco (Nicotina tabacum). A cosmid clone carrying 16 kilobases (kb) of contiguous genomic DNA partially complements this mutant. Altered growth of the mutant in planta was also partially restored. Marker exchange mutagenesis with Tn3- HoHo1 at two other sites within this locus results in mutants with attenuated and severely reduced pathogenicity. The locus is complex and contains repetitive DNA sequences. Northern analysis reveals that this locus is expressed in planta, but is not expressed in a rich growth medium, and the transcript is larger than 10 kb, suggesting that the locus is transcribed as a polycistronic mRNA. Comparison of total cellular protein profiles of R32 and PS9024 using SDS-PAGE analysis further reveals that at least nine protein bands ranging from approximately 100 kD or above in size are present in the wild type strain R32, but absent from the mutant. Additionally, a protein of approximately 45 kD is absent from the mutant. The site of Tn5 insertion has been partially sequenced. The initial search of the data banks suggested a gene or genes related to the ornithine biosynthetic pathway map to this locus. Further study strongly suggest a gene that encodes a membranceassociated protein and under the control of a promoter identical to appA gene promoter maps at this site and it is involved in the process of pathogenesis.
Author: Hanh Ngoc Lam
Publisher:
ISBN:
Category :
Languages : en
Pages : 474
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The type III secretion system (T3SS) is required for virulence of the gram-negative plant pathogen Pseudomonas syringae pv. tomato DC3000 (DC3000) in tomato and Arabidopsis. The alternative sigma factor HrpL directly regulates expression of T3SS genes by binding to a short DNA sequence designated as the "hrp promoter". The ability of DC3000 to colonize plants, subdue multiple layers of plant defense and multiply in plant tissues relies on the activities carried out by the many T3SS regulon members (known collectively as hrp genes). Efforts to identify genes involved in pathogenicity were initiated over three decades ago. However, HrpL binding to hrp promoters has never been directly demonstrated and it is unclear if the list of HrpL-regulated genes is complete. The first goal of the research described here was to systemically and exhaustively identify HrpL-binding sites and likely hrp promoters in the DC3000 genome. Employing chromatin immuno-precipitation, coupled with high-throughput sequencing (ChIP-Seq) and transcription start site analysis (modified RNA-Seq), we found twenty sites representing novel hrp promoters. Using deletion analysis, we attempted to determine if the genes downstream from a subset of these promoters could be linked to virulence. However, the deletions did not affect the hypersensitive response or in planta growth of the resulting strains. Interestingly, many new HrpL regulon members appear to be unrelated to the T3SS (based on their annotations), and orthologs for some of these can be identified in non-pathogenic bacteria. The connection of these new HrpL regulon members to virulence is not obvious. The HrpL regulon is activated as a result of a chain of events, most of which are not well understood. It is known that RpoN, which controls the transcription of hrpL in DC3000, is required for virulence in several bacterial species. Motivated by the hypothesis that genes are coordinately regulated in order to serve a strategic purpose (e.g., virulence), our second goal was to look for other genes activated by RpoN in parallel with hrpL. RpoN ([sigma]54) requires specialized enhancer-binding proteins (EBPs) in order to activate transcription. This arrangement presumably allows the cell to respond to environmental signals by modifying the transcription of particular genes. Using ChIP-Seq and RNA-Seq, we identified candidate RpoN-dependent genes as well as genes that were differentially expressed under hrp-inducing conditions. This initial survey includes more than 200 likely RpoN-regulated genes involved in flagella biosynthesis, energy metabolism, nitrogen metabolism, transport and binding proteins, and small noncoding RNAs, as well as putative regulatory proteins and EBPs. Among the genes that were differentially regulated between hrp-inducing and repressing conditions, more than one dozen appear to be regulated by RpoN and are therefore potentially important in functions related to plant association or virulence.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 632
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