Investigation of the Applications of Reverse-phase High Performance Liquid Chromatography in the Structural Studies of Collagen-like Model Peptide PDF Download
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Author: Shih Tak Khew
Publisher:
ISBN:
Category : Chemical engineering
Languages : en
Pages :
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Author: Shih Tak Khew
Publisher:
ISBN:
Category : Chemical engineering
Languages : en
Pages :
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Author: James M. Sulzberger (Jr.)
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 174
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"Analytical characterization of biomolecules such as proteins is inherently more complex than its traditional counterpart, traditional small molecule pharmaceuticals. While it is possible to develop and validate one or two assays with one or two orthogonal methods to assess specificity and stability with regards to product related degradants, proteins require many. Typically, a combination of reversed phase, ion-exchange, gel electrophoresis, and size exclusion chromatography to evaluate charged variants, glycoform variants, and size variant product related impurities in drug substance and drug product. Both top-down and bottom- up approaches for analytical characterization of proteins are critical for ensuring primary, secondary, tertiary, and quaternary structure to be certain of the safety and efficacy of a molecule, as one type of degradation or impurity can be detrimental to either. HPLC and UPLC of intact protein as a top-down approach, as well as a corresponding bottom-up method known as peptide mapping, where digestion of a protein after reduction and alkylation using a specific chemical or enzyme is used to look into the location of post translational modifications, are both employed. When developing a peptide map, a critical parameter is recovery and stability of digested protein. In this study, human serum albumin is uses as a model protein for illustration of sensible approach for evaluation of these two criteria with the development and validation of a reversed phase chromatography assay for evaluation of protein recovery and stability with regards to aggregation formation."--
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1556
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Author: H. B. Patel
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Author: Gábor Szepesi
Publisher: Wiley-VCH
ISBN:
Category : Science
Languages : en
Pages : 376
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Author: Otakar Mikeš
Publisher: Elsevier Science & Technology
ISBN:
Category : Science
Languages : en
Pages : 754
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Book Description
Proteins and their higher molecular weight fragments -- Enzymes -- Peptides -- Nucleic acids, polynucleotides and oligonucleotides -- Polysaccharides and oligosaccharides -- Compound biopolymers and biooligomers -- Applications of high-performance liquid chromatographic methods in structural studies of biopolymers and biooligomers -- Register of biopolymers, biooligomers fragments separated by HPLC or MPLC methods -- Bibliography -- Prospects for high-pressure and medium-pressure column liquid chromatography of biopolymers and biooligomers.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1384
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Author: William S. Hancock
Publisher: CRC-Press
ISBN: 9780849335112
Category : Science
Languages : en
Pages : 544
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Book Description
All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.
Author: Anthony W. Purcell
Publisher:
ISBN:
Category : High performance liquid chromatography
Languages : en
Pages : 594
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Author:
Publisher:
ISBN:
Category : Chemical engineering
Languages : en
Pages : 940
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